Construction of a Plasmid Vector for Expression of Bacteriocin N15-encoding Gene and Effect of Engineered Bacteria on Enterococcus Faecalis
Keywords:
EntAI, Enterococci, Shuttle vector, pIL253Abstract
A 6.09-kb plasmids vector pOri253 was constructed from the plasmid pIL253 (5.2 kb) and a 0.89-kb fragment of oriColE1 from pBluescript II KS. The bifunctional plasmid pOri253 conferred erythromycinresistance in both Escherichia coli and Enterococcus faecalis. It has a unique site for each of EcoRI, BamHI, SalI and PstI derived from pIL253. The plasmid is quite stable in E. faecalis JCM8726 when cultured in Mann Rogosa Sharpe broth without antibiotic. The lactococcal promoter P23 was inserted at one end of the pOri253 multicloning site. Gene expression was assessed by using an entAI gene which coded for bacteriocin N15. The E. faecalis harboring constructed plasmid carrying P23 (pOrient23) had more antibacterial activity than parental E. faecalis JCM8726 or its clone harboring non-P23 containing plasmid (pOrient), as determined by means of an overlay method.Downloads
Metrics
References
Stiles ME. Biopreservation by lactic acid bacteria. Antonie van Leeuwenhoek 1996; 70: 331-45.
Sorvig E Gronqvist S Naterstad K Mathiesen G Eijsink VGH Axelsson L. Construction of vectors for inducible gene expression in Lactobacillus sakei and L. plantarum. FEMS Microbiol 2003; 229: 119-26.
Alender M Desmet I Nollet L Verstraete W von Wright A Mattila-Sandholm T. The effect of probiotic strains on the microbiota of the stimulator of the Human Intestinal Microbial Ecosystem (SHIME). Int J Food Microbial 1999; 46: 71-9.
Schultz M Veltkamp C Dieleman LA Grenther WB Wyrick PB Tonkonogy SL Sartor RB. Lactobacillus plantarum 299V in the treatment and prevention of spontaneous colitis in interleukin-10-deficient mice. Inflmm Bowel Dis 2002; 8: 71-80.
Ryane MP Meaney WJ Ross RP Hill C. Evaluation of lacticin 3147 and a teat seal containing this bacteriocin for inhibition of mastitis pathogens. Appl Environ Microbiol 1998; 64: 2287-90.
Klaenhammer TR. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol 1993; 12: 39-86.
Nes IF Diep DB Havarstein LS Brurberg MB Eijsink V Holo H. Biosynthesis of bacteriocins in lactic acid bacteria. Antonie van Leeuwenhoek 1996; 70: 113-28.
Allison GE Worobo RW Stiles ME Klaenhammer TR. Heterologous expression of the lactacin F peptides by Carnobacterium piscicola LV17. Appl Environ Microbiol 1995; 61: 1371-7.
Chikindas ML Venema K Ledeboer AM Venema G Kok J. Expression of lactococcin A and pediocin PA-1 in heterologous host. Lett Appl Microbiol 1995; 21: 183-9.
van Belkum MJ Worobo RW Stiles ME. Double glycine type leader peptides direct secretion of bacteriocin by ABC transporter: colicin V secretion in Lactococcus lactis. Mol Microbiol 1997; 23: 1293-301.
Brurberg MB Nes IF Eijsink VGH. Pheromone induced production of antimicrobial peptides in Lactobacillus. Mol Microbiol 1997; 26: 347-60.
Horn N Martinez MI Martinez JM Hernandez PE Gasson MJ Rodriguez JM Dodd HM. Production of pediocin PA-1 by Lactococcus lactis using the lactococcin A secretory apparatus. Appl Environ Microbiol 1998; 64: 818-23.
Miller KW Schamber R Chen YL Ray B. Production of active chimeric pediocin AcH in Escherichia coli in the absence of processing and secretion genes from the Pediococcus pap operon. Appl Environ Microbiol 1998; 64: 14-20.
Fernandez M van Doesburg W Rutten GA Marugg JD Alting AC van Kranenburg R Kuipers OP. Molecular and functional analyses of the metC gene of Lactococcus lactis, encoding cystathionine beta-lyase. Appl Environ Microbiol 2000; 66: 42-8.
Sakamoto K Margolles A van Veen HW Konings WN. Hop resistance in the beer spoilage bacterium Lactobacillus brevis is mediated by the ATP-binding cassette multidrug transporter HorA. J Bacteriol 2001; 183: 5371-5.
de Ruyter PGGA Kuipers OP de Vos WM. Controlled gene expression systems for Lactobacillus lactis with the food grade inducer nisin. Appl Environ Microbiol 1996; 62: 3662-7.
Axelsson L Katla T Bjornslett M Eijsink VGH Holck A. A system for heterologous expression of bacteriocins in Lactobacillus sake. FEMS Microbiol Lett 1998; 168: 137-43.
Losteinkit C Uchiyama K Ochi S Takakoya T Nagahisa K Shioya S. Characterization of Bacteriocin N15 produced by Enterococcus faecium N15 and cloning of the related genes. J Biosci Bioeng 2001; 91: 390-5.
Que YA Haefliger JA Francioli P Moreillon P. Expression of Staphylococcus aureus clumping factor A in Lactobacillus lactis subsp. cremoris using a new shuttle vector. Infect Immun 2000; 68: 3516-22.
Sambrook J Fritsch EF Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989.
Simon D Chopin A. Constrution of a vector plasmid family and its use for molecular cloning in Streptococcus lactis. Biochimie 1988; 70: 559-66.
Macrina FI Tobian JA Jones KR Evans RP Clewell DB. A cloning vector able to replicate in Escherichia coli and Streptococcus sanguis. Gene 1982; 19: 345-53.
van der Vossen JM van der lelie D Venema G. Isolation and characterization of Streptococcus cremoris Wg-2specific promoters. Appl Environ Microbiol 1987; 53: 2452-7.
Gruss A Ehrlich D. Insertion of foreign DNA into plasmids from Grampositive bacteria induces formation of high-molecular-weight plasmid multimers. J Bacteriol 1988; 3: 1183-90.
Mari YM Espinoza AES Ubieta R Batista OF Fernandez MT. Effect of the selection marker on the viability and plasmid stability of two human proteins with neurotrophic action expressed in Escherichia coli. Biochem Biophysic Res Commu 1999; 258: 29-31.
Old RW Primrose SB. Principles of gene manipulation. 4th ed. Oxford: Blackwell Scientific Publications. 1989.
Brigidi P Gonzalez-Vara Y RA Rossi M Matteuzzi D. Study of stability of recombinant plasmids during the continuous of Bacillus sterothermophilus NUB3621 in nonselective medium. Biotechnol Bioengineer 1996; 53: 507-14.
Gamel PH Piot JC. Characterization and properties of a novel plasmid vector for Bacillus thuringiensis displaying compatibility with host plasmids. Gene 1992; 120: 17-26.
de Vos WM. Gene expression systems for lactic acid bacteria. Curr Opin Microbiol 1999; 2: 289-95.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2011 Walailak University
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
