Assessment of Optimal Infusion Condition for Thunbergia laurifolia Tea by using Response Surface Methodology


  • Piyanuch ROJSANGA Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand
  • Puriwat JIEWATAKUNTUM Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand
  • Waree LIMWIKRANT Department of Manufacturing Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand
  • Kotchaphan CHOOLUCK Department of Manufacturing Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand



Caffeic acid, Infusion condition, Response surface methodology, Rosmarinic acid, Thunbergia laurifolia


According to the National List of Essential Medicines of Thailand, the Thunbergia laurifolia (TL) tea is categorized as antipyretic and detoxifying herbal medicine. This tea has also been used for the treatment of drug addiction and smoking cessation. However, suggested infusion conditions were varied which may result in variations in extracted active contents and bioactivities. In this study, the infusion condition that maximized extraction of caffeic acid (CA) and rosmarinic acid (RA), the major markers of TL tea, was assessed by using response surface methodology (RSM). The contents of markers were analyzed by a validated high-performance liquid chromatographic method. To evaluate the extraction efficiency, the marker contents obtained by preparing the tea under the optimal condition were compared to those obtained by aqueous extraction process. The results demonstrated that the marker contents varied considerably according to infusion variables; time, temperature and volume (150 - 250 mL). By using RSM, it was successfully found that the optimal condition for the tea (3 g) was as follows: 200 mL of boiling water and infusion time of 10 min. More importantly, by using infusion method, the major portion of markers remained in the tea powder implying poor extraction efficiency of the method. This result suggested that the use of TL extract would be benefit in terms of quality and efficacy. The optimal infusion condition could be utilized in clinical studies to find an effective oral dose range of markers. Ultimately, the obtained information could be used to determine the oral dose of TL extract.


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National List of Essential Medicines, Available at:, acessed May 2018.

S Ussanawarong and T Thesiri. Effect of Thunbergia laurifolia Linn. on detoxication of parathion in rat. Khon Kaen Univ. Res. J. 2001; 6, 3-13.

J Tangpong and S Satarug. Alleviation of lead poisoning in the brain with aqueous leaf extract of the Thunbergia laurifolia (Linn.). Toxicol. Lett. 2010; 198, 83-8.

W Ruangyuttikarn, P Chattaviriya, N Morkmek, S Chuncharunee and N Lertprasertsuke. Thunbergia laurifolia leaf extract mitigates cadmium toxicity in rats. Scienceasia 2013; 39, 19-25.

P Pramyothin, H Chirdchupunsare, A Rungsipipat and C Chaichantipyuth. Hepatoprotective activity of Thunbergia laurifolia Linn extract in rats treated with ethanol: In vitro and in vivo studies. J. Ethnopharmacol. 2005; 102, 408-11.

O Wonkchalee, T Boonmars, C Aromdee, P Laummaunwai, W Khunkitti, K Vaeteewoottacharn, P Sriraj, R Aukkanimart, W Loilome, Y Chamgramol, C Pairojkul, Z Wu, A Juasook and P Sudsarn. Anti-inflammatory, antioxidant and hepatoprotective effects of Thunbergia laurifolia Linn. on experimental opisthorchiasis. Parasitol. Res. 2012; 111, 353-9.

P Suwanchaikasem, C Chaichantipyuth and S Sukrong. Antioxidant-guided isolation of rosmarinic acid, a major constituent, from Thunbergia laurifolia and its use as a bioactive principle for standardization. Chiang Mai J. Sci. 2014; 41, 117-27.

N Ruangpayungsak. 2016, Development of HPLC method for fingerprinting and quantitative analysis of active compounds of Thunbergia Laurifolia leaf extract using chemometric analysis. M.S. Dissertation, Mahidol University, Bangkok, Thailand.

VR Coelho, CG Vieira, LP de Souza, F Moyses, C Basso, DK Papke, TR Pires, IR Siqueira, JN Picada and P Pereira. Antiepileptogenic, antioxidant and genotoxic evaluation of rosmarinic acid and its metabolite caffeic acid in mice. Life Sci. 2015; 122, 65-71.

P Rojsanga, P Sithisarn, K Tanaka, D Mizuki and K Matsumoto. Thunbergia laurifolia extract ameliorates cognitive and emotional deficits in olfactorectomized mice. Pharm. Biol. 2015; 53, 1141-8.

S Kondo, AE Omri, J Han and H Isoda. Antidepressant-like effects of rosmarinic acid through mitogen-activated protein kinase phosphatase-1 and brain-derived neurotrophic factor modulation. J. Funct. Foods. 2015; 14, 758-66.

W Thongsaard and C Marsden. Effect of Thunbergia laurifolia extract on extracellular dopamine level in rat nucleus accumbens. J. Med. Assoc. Thai. 2013; 96, 85-9.

W Thongsaard, R Sangpayap and C Marsden. Chronic effect of Thunbergia laurifolia extract and cocaine in rats using behavior model of addiction. J. Med. Assoc. Thai. 2015; 98, 48-52

Rosmarinic acid for smoker dehabituation, Available at:, acessed May 2018.

Agent For Smoker Dehabituation, Available at:, accessed May 2018.

L Barros, M Duenas, MI Dias, MJ Sousa, C Santos-Buelga and IC Ferreira. Phenolic profiles of cultivated, in vitro cultured and commercial samples of Melissa officinalis L. infusions. Food Chem. 2013; 136, 1-8.

TFF da Silveira, AD Meinhart, CA Ballus and HT Godoy. The effect of the duration of infusion, temperature, and water volume on the rutin content in the preparation of mate tea beverages: An optimization study. Food Res. Int. 2014; 60, 241-5.

E Sharpe, F Hua, S Schuckers, S Andreescu and R Bradley. Effects of brewing conditions on the antioxidant capacity of twenty-four commercial green tea varieties. Food Chem. 2016; 192, 380-7.

Z Nikniaz, R Mahdavi, SJ Ghaemmaghami, N Lotfi Yagin and L Nikniaz. Effect of different brewing times on antioxidant activity and polyphenol content of loosely packed and bagged black teas (Camellia sinensis L.). Avicenna J. Phytomed. 2016; 6, 313-21.

JT DiPiro. Concepts in Clinical Pharmacokinetics. American Society of Health-System Pharmacists, Bethesda, 2010.

SLC Ferreira, RE Bruns, HS Ferreira, GD Matos, JM David, GC Brandao, EGP da Silva, LA Portugal, PS dos Reis, AS Souza and WNL dos Santos. Box-Behnken design: An alternative for the optimization of analytical methods. Anal. Chim. Acta. 2007; 597, 179-86.

European Pharmacopoeia Commission. European Pharmacopoeia. 8th (eds.), Council of Europe, Europe, 2013.

Association of official analytical chemistry (AOAC). Guidelines for single laboratory validation of chemical methods for dietary supplements and botanicals, Available at:, accessed May 2018.

MF Nishiyama, MAF Costa, AM Costa, CGM Souza, CG Bôer and CK Bracht. Brazilian green tea (Camellia sinensis var assamica): Effect of infusion time, mode of packaging and preparation on the extraction efficiency of bioactive compounds and on the stability of the beverage. Ciênc. Tecnol. Aliment. 2010; 30, 191-6.

JE Cacace and G Mazza. Mass transfer process during extraction of phenolic compounds from milled berries. J. Food Eng. 2003; 59, 379-89.

JR Vergara-Salinas, J Perez-Jimenez, JL Torres, E Agosin, JR Perez-Correa. Effects of temperature and time on polyphenolic content and antioxidant activity in the pressurized hot water extraction of deodorized thyme (Thymus vulgaris). J. Agric. Food Chem. 2012; 60, 10920-9.

SA Makanjuola. Influence of particle size and extraction solvent on antioxidant properties of extracts of tea, ginger, and tea-ginger blend. Food Sci. Nutr. 2017; 5, 1179-85.

YS Lin, YJ Tsai, JS Tsay and JK Lin. Factors affecting the levels of tea polyphenols and caffeine in tea leaves. J. Agric. Food Chem. 2003; 51, 1864-73.

CI Heck and EG de Mejia. Yerba mate tea (Ilex paraguariensis): A comprehensive review on chemistry, health implications, and technological considerations. J. Food Sci. 2007; 72, 138-51.

S Govindaraghavan, NJ Sucher. Quality assessment of medicinal herbs and their extracts: Criteria and prerequisites for consistent safety and efficacy of herbal medicines. Epilepsy Behav. 2015; 52, 363-71.

SP Ishwarya, C Anandharamakrishnan and AGF Stapley. Spray-freeze-drying: A novel process for the drying of foods and bioproducts. Trends Food Sci. Technol. 2015; 41, 161-81.




How to Cite

ROJSANGA, P. ., JIEWATAKUNTUM, P. ., LIMWIKRANT, W. ., & CHOOLUCK, K. . (2020). Assessment of Optimal Infusion Condition for Thunbergia laurifolia Tea by using Response Surface Methodology. Walailak Journal of Science and Technology (WJST), 17(10), 1077–1087.