Screening, Identification and Antibacterial Activities of Effective Thermotolerant Bacillus spp. Strains Isolated from Raw Milk



Forty-one isolates of Bacillus species were isolated from raw milk, analyzed using the spot on lawn and agar diffusion method in terms of their general inhibition effects to test bacteria (Escherichia coli TISTR 887 and Staphylococcus aureus TISTR 517). The results demonstrated that most isolates are effective against Gram-positive and Gram-negative bacteria whereas their extensive inhibition effect is particularly against Gram-positive bacteria. Only 2 effective thermotolerant isolates, BA8 and BA16, exerted broad spectrum antibacterial activities against both test bacteria.  Based on biochemical and physiological properties, they were classified as Brevibacillus laterosporus and Geobacillus thermoglucosidasius, respectively.


Bacillus species, Escherichia coli, Staphylococcus aureus

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M Morikawa, M Ito and T Imanaka. Isolation of a new surfactin producer Bacillus pumilus A-1, and cloning and nucleotide sequence of the regulator gene, psf-1. J. Ferment. Bioeng. 1992; 74, 255-61.

C Perez, C Suarez and GR Castro. Production of antimicrobials by Bacillus subtilis MIR15. J. Biotechnol. 1992; 26, 331-6.

C Perez, C Suarez and GR Castro. Actimicrobial activities determined in strains of Bacillus circulans cluster. Folia Microbiol. 1993; 38, 25-8.

F Drablos, D Nicholson and M Ronning. EXAFS study of zinc coordination in Bacitracin A. Biochim. Biophys. Acta. 1999; 1431, 433-42.

R Eltem and F Ucar. The determination of antimicrobial activity spectrum of 23 Bacillus strains isolated from Denizli-Acigol (Bitter Lake) which is soda lake (Na2SO4). J. KUKEM 1998; 21, 57-64.

S Steller, D Vollenbroich, F Leenders, T Stein, B Conrad, J Hofemeisterr, P Jaques, P Thonart and J Vater. Structural and functional organization of the fengicin synthease multienzyme system from Bacillus subtilis b213 and A1/3. Chem. Biol. 1999; 6, 31-41.

JL Milner, SJ Raffel, BJ Lethbridge and J Handelsman. Culture conditions that influence accumulation of zwittermicin a by Bacillus cereus UW85. Appl. Microbiol. Biotechnol. 1995; 43, 685-91.

F Peypoux, JM Bonmatin and J Wallach. Recent trends in the biochemistry of surfactin. Appl. Microbiol. Biotechnol. 1999; 51, 553-63.

A Galvez, M Maqueda, P Cordovilla, M Martinez-Bueno, M Lebbadi and E Valdivia. Characterization and biological activity against Naegleria fowleri of amonebicins produced by Bacillus licheniformis D-13. Antimicrob. Agents Chemother. 1994; 38, 1314-9.

PHA Sneath. Endospore-forming Gram-positive rods and cocci. In: PHA Sneath, NS Mair, ME Sharpe and JG Holt (eds.). Bergey’s Manual of Systematic Bacteriology, Vol II. Williams & Wilkins, Baltimore, 1986, p. 1104-39.

BH Cadirci and S Citak. A comparison of two methods used for measuring antagonistic activity of lactic acid bacteria. Paki. J. Nutri. 2005; 4, 237-41.

JA Reinheimer, MR Demkov and MC Condioti. Inhibition of coliform bacteria by lactic cultures. Aust. J. Daily Technol. 1990; May, 5-9.

B Aslim, N Saglam and Y Beyatli. Determination of some properties of Bacillus isolated from soil. Turk. J. Biol. 2002; 26, 41-8.

M Yilmaz, H Soran and Y Beyatli. Antimicrobial activities of some Bacillus spp. strains isolated from the soil. Microbiol. Res. 2006; 161, 127-31.

K Chawawisit and M Lertcanawanichakul. Minimum inhibitory concentration (MIC) of crude preparations of Brevibacillus laterosporus SA14 bioactive material compared to vancomycin and oxacillin, against clinical isolates of methicillin-resistant Staphylococcus aureus. World J. Microbiol. Biotechnol. 2008, in press.


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