Isolation and Selection of Anti-Candida albicans Producing Lactic Acid Bacteria
Keywords:Lactic acid bacteria, Antifungal activity
AbstractThe forty isolates of lactic acid bacteria (LAB) were obtained from various fermented foods. The cross streak plate method was used to preliminary screen for antimicrobial activity. LAB were isolated by selective medium, Mann Rogosa Sharpe (MRS). Most of the isolates showed inhibition against Staphylococcus aureus TISTR 517, Bacillus subtilis TISTR 008, Micrococcus luteus TISTR 884, Escherichia coli TISTR 887, Pseudomonas aeruginosa TISTR 781, and Candida albicans DMST 5239. Only sterile culture supernatant of isolate No. L14, later identified as Lactococcus lactis, showed antifungal activity by means of agar well diffusion assay. The activity was stable during heat treatment and was retained even after autoclaving at 121 °C for 15 minutes. Maximum activity was observed at pH values between 2.5-4.0, and was lost at higher pH values. The anti-C. albicans activity was fully regained after readjustment of the pH to the initial value (pH 3.5).
Cox G Perfect J. Fungal Infections. Curr Opin Infect Dis 1993; 6: 422-6.
Fox JL. Fungal infection rates are increasing. ASM News 1993; 59: 515-8.
Herbrecht R. The changing epidemiology of fungal infections: are the lipid-based of amphotericin B and advance? Eur J Haematol 1996; 56: 12-7.
Legan JD. Mould spoilage of bread : the problem and some solutions. Int Biodeterrior Biodagrad 1993; 32: 33-53.
Selitrennikoff CP. Antifungal proteins. Appl Environ Microbiol 2001; 67(7): 2883-94. 6) Rocken W. Applied aspects of sourdough fermentation. Adv Food Sci 1996; 18: 212-6.
Corsetti A Gobbetti M Rossi J Damiani P. Antimould activity of sourdough lactic acid bacteria: identification of a mixture of organic acids compounds by Lactobacillus sanfrancisco CB1. Appl Microbiol Biotechnol 1998; 50: 253-6.
Gourama H. Inhibition of growth and mycotoxin production of Penicillium by Lactobacillus species. Lebensm-Wiss Tech 1997; 30: 279-83.
Niku-Paavola ML Laitila A Mattila-Sandholm T Haikara A. New types of antimicrobial compounds produced by Lactobacillus plantarum. J appl Microbiol 1999; 86: 29-35.
Dodd HM Gasson MJ. Bacteriocins of lactic acid bacteria. In: Gasson MJ De Vos WM (eds), Genetics and Biotechnology of Lactic Acid Bacteria. Blackie Academic & Professional, London, England, 1994, p. 211-51.
Franklan R Elliott A. Identification, classification and clinical relevance of catalase negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Mirobiol Rev 1995; 8: 479-95.
Mundt JO. Lactic and streptococci. In: Sneath PHA Mair NS Sharpe ME Holt G (eds), Bergey=s Manual of Systematic Bacteriology, vol 2, Baltimore, MD: Williams and Wilkins, 1986, p. 1065-6.
Villani F Aponte M Blaiotta G Mauriello G Pepe O Moschetti G. Detection and characterization of a bacteriocin, garviecin L1-5. produced by Lactococcus garvieae isolated from raw cow=s milk. L appl Micobiol 2001; 90: 430-9.
Kandler O Weiss N. Genus Lactobacillus Beijerinck 1901, 212AL. In: Sneath PHA Mair NS Sharpe ME Holt JG (eds), Bergey=s Manual of Systematic Bacteriology, 10th ed. Baltimore, MD: Williams and Wilkins, 1986, p. 1209-34.
Biswas SR Ray P Johnson MC Ray B. Influence of growth conditions on the production of a bacteriocin, pediocin AcH, by Pediococcus acidilactici H. Appl Environ Microbiol 1991; 57: 1265-7.
Levitz S. Overview of host defenses in fungal infections. Clin Infect Dis 1992; 14: 37-42.
Pfaller M Wenzel R. Impact of the changing epidemiology of fungal infections in the 1990=s. Eur J Clin Microbiol Dis 1992; 11: 287-91.
Bounaix S Benachour A Novel G. Presence of lactose gene and insertion sequences in plasmids of minor species of the genus Lactococcus. Appl Envicon Microbiol 1996; 62: 112-5.
Kimoto H Nomura M Kobayashi M Okamoto T Ohmomo S. Identification and probiotic characteristics of Lactococcus strains from plant materials. JARQ 2004; 38: 111-7.
Magnusson J Schnurer J. Lactobacillus coryniformis subsp. Coryniformis strain Si3 produced a broad-spectrum proteinaceous antifungal compound. Appl Eniron Microbiol 2001; 67(1): 1-5.
Liu W Hansen JN. Some chemical and physical properties of nisin, a smallprotein antibiotic produced by Lactococcus lactis. Appl Environ Microbiol 1990; 56: 2551-8.
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