In Vitro Germplasm Conservation of Garcinia mangostana L. and Lansium domesticum Corr.


  • Wararat KEATMETHA School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161
  • Padungsak SUKSA-ARD School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161
  • Maruay MEKANAWAKUL School of Science, Walailak University,Thasala, Nakhon Si Thammarat 8016ๅ
  • Sompong TE-CHATO Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla 90110


Germplasm conservation, Garcinia mangostana L., Lansium domesticum Corr., growth retardants, tissue desiccation treatment


The in vitro germplasm conservation of mangosteen (Garcinia mangostana L.) and longkong (Lansium domesticum Corr.) was investigated by suppression of shoot growth using growth retardants and increasing the osmotic potential of culture medium. Abscisic acid (ABA) at 2 mg/l affected to suppress shoot growth of mangosteen and longkong more than paclobutrazol. Although shoot growth of both plants was consequently inhibited for 12 months of conservation, the regrowth ability was successfully obtained when transferred to proliferation medium. Besides, increasing the concentration of agar up to 14 g/l was found to be more effective to suppress shoot growth of mangosteen and longkong by treatment of tissue desiccation. Severe succulence of mangosteen shoot was observed when shoots were cultured in a medium containing mannitol.


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How to Cite

KEATMETHA, W., SUKSA-ARD, P., MEKANAWAKUL, M., & TE-CHATO, S. (2011). In Vitro Germplasm Conservation of Garcinia mangostana L. and Lansium domesticum Corr. Walailak Journal of Science and Technology (WJST), 3(1), 33–50. Retrieved from



Research Article