Effects of Rooting Substrates on In Vitro Rooting of Anthurium andraeanum L. cv. Avanti
Keywords:Anthurium andraeanum L., Rooting substrates, Agar Phytagel, Peat moss, Sphagnum moss, Vermiculite
AbstractA study was made of the effects of rooting substrates on in vitro rooting of Anthurium andraeanum L. cv. Avanti, orange flower. Initiation of root was attempted in several rooting substrates with modified ½ MS medium supplemented with 30 g/l sucrose. The cut end of the shoot was dipped in 2.5 g/l indole-3-butyric acid (IBA) before insertion in substrates. After 4 weeks of culture, it was found that roots were markedly induced in 8 and 12 g/l agar with 86.67 and 73.33% in root induction rate, 14.62 and 12.41 mm in root length, with 3.54 and 3.91 roots in root number respectively. However, 93.33% of root induction rate with 3.00 roots, and 11.66 mm in root length were produced on medium containing 1.5 g/l phytagel while peat moss and vermiculite could induce rooting at 46.67% with 7.86 and 6.66 mm in length, with 1.00 and 1.86 roots respectively. Root could not be induced in sphagnum moss. Anatomical study of root showed no abnormality in all rooting substrates. The roots formed in high concentrations of agar at 12 and 16 g/l, 2.0 and 2.5 g/l phytagel, and peat moss were thicker than those formed in other rooting substrates. Especially, root formed in peat moss had the largest vascular diameter. However, roots formed in phytagel, peat moss, and vermiculite had more root hairs than those formed in agar substrate. Plantlets, rooted in peat moss and vermiculite, survived at 100% after acclimation in a mixture of soil and coconut husk.
Fure CC Adelheid RK Nellie S. Anthurium roots for micropropagation and Agrobacterium tumefaciens-mediated gene transfer. Plant Cell, Tissue and Organ Cult 1997; 49(1): 71-4.
Martin KP Joseph D Madarrery J Philip VJ. Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort. In: Vitro Cellular and Developmental Biology-Plant [online] 2003; 39(5): 500-4. [cited 14 January 2005]. Available from: http://www.bioone.org/bioone/?request=getdocument&issn=1054-5476&volume=039&issue=05&page=0500.
Whei-Lan T. Regeneration of t Anthurium adventitious shoots using liquid or raft culture. Plant Cell, Tissue and Organ Cult 1997; 49(2): 153-6.
Suksa-Ard P Kataoka I Fujime Y Beppu K Subhadrabandhu S. Development of rooting system for tissue cultured papaya shoots using rockwool blocks. Jpn J Trop Agric 1998; 42: 119-21.
Kataoka I. Influence of rooting substrates on the morphology of papaya root formed in vitro. Jpn J Trop Agric 1994; 38: 251-7.
Gabriel BL Nelson LF Gérson RDLF. Use of vermiculite as a substrate and effect of light on in vitro rooting of pears, cv. Bartlett and clone OH´F97. Ciênc agrotec 2002; 26: 977-82.
Murashige T Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 1962; 15: 473-97.
McClelland MT Smith MAL Carothers ZB. The effects of in vitro and ex vitro root initiation on subsequent microcutting root quality in three woody plants. Plant Cell, Tissue Organ Cult 1990; 23: 115-23.
Hu CY Wang PJ. Meristem, shoot tip, and bud culture. In: Evans DA Sharp WR Ammarito PV Yamada Y (eds). Handbook of Plant Cell Culture, Vol. 1 Macmillan Publishing Co., USA, 1983, p. 177-227.
Teo CKH Chen LK. The effects of agar content, nutrient concentration, genotype and light intensity on the in vitro rooting of papaya microcuttings. J Hort Sci 1994; 69: 267-73.
Yu TA Yeh SD Cheng YH Yang JS. Efficient rooting for establishment of papaya plantlets by micropropagation. Plant Cell, Tissue and Organ Cult 2000; 61: 29-35.
Zimmerman TW Broome OC. Micropropagation of woody plant: post tissue cultures aspects. Acta Hort 1989; 22: 489-99.
Hutchinson JF. Factors affecting shoot proliferation and root initiation in organ cultures of the apple ‘Northern Spy’. Sci Hort 1984; 22: 347-58.
Kirdmanee C Altman A Ziv M Izhar S. Plant biotechnology and in vitro biology in the 21st century. Curr Plant Sci Biot 1999; 36: 651-4.
Kirdmanee C Kitaya Y Kozai T. Effects of CO2 enrichment and supporting material on growth, photosynthesis and water potential of Eucalyptus shoots/plantlets cultured photoauto-trophically in vitro. Environ Control in Biol 1995; 33: 133-41.
How to Cite
Copyright (c) 2011 Walailak University
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.