Alteration of Spathoglottis eburnea Gagnep. Ploidy Level after Colchicine Treatments

Authors

  • Witayaporn PORNCHUTI Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400
  • Kanchit THAMMASIRI Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400
  • Ngarmnij CHUENBOONNGARM Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400
  • Nathinee PANVISAVAS Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400

Keywords:

Mixoploid, flow cytometry, chromosome count, stomata analysis

Abstract

Improvement of Spathoglottis eburnea Gagnep (Orchidaceae) with alterative morphology was performed and observed by polyploidy induction. Two millimeters long shoot tips were immersed and cultured in ½ MS liquid medium supplemented with 0, 0.25, 0.5, 0.75, and 1 % (w/v) of sterilized colchicine solutions for 1 d. After 3 months of culture, the highest polyploidy induction was obtained with 1 % (w/v) colchicine for 1 d, which gave a shoot survival rate of about 45 % and which regenerated into 40 % polyploid plantlets. Five polyploid plantlets were further maintained by subculturing every 2 months on ½ MS agar medium supplemented with 8 g L-1 activated charcoal. After the third subculture, all of them were morphological and cytological chimeras, which had alterations in leaf morphology. The variation of ploidy level was determined again by flow cytometry and chromosome counting. A flow cytrometry showed that tetraploid plantlets changed to mixoploid plants, where phenotype instability was further observed, in correlation with chromosome instability. The density of stomata and the stoma index were observed and showed no significant differences between mixoploids and diploids. However, the guard cell size of mixoploids was larger than those of diploids. In the light of our findings, we can consider polyploidy induction to be an effective technique for plant improvement; however, there is a necessity to study genetic stability between the clones after micropropagation.

 

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

A Pridgeon. The Illustrated Encyclopedia of Orchids. Timber Press, Portland, 2003.

O Lense. Biological screening of selected traditional medicinal plants species utilized by local people of Manokwari, West Papua Province. Bioscience 2011; 3, 145-50.

PAKX Silva, S Callegari-Jacques and MH Bodanese-Zanettini. Induction and identification of polyploids in Cattleya intermedia Lindl. (Orchidaceae) by in vitro techniques. Ciência Rural. 2000; 30, 105-11.

S Wannakrairoj and W Tefera. In vitro chromosome doubling in Korarima [Aframomum corrorima (Braun) P.C.M. Jansen] using colchicine and oryzalin. Kasetsart J. (Nat. Sci.). 2013; 47, 684-94.

M Nimura, J Kato, H Horaguchi, M Mii and K Sakai. Induction of fertile amphidiploids by artificial chromosome-doubling in interspecific hybrid between Dianthus caryophyllus L. and D. japonicas Thumb. Breed. Sci. 2006; 56, 303-10.

H Hosseini, M Chehrazi, MM Sorestani and D Ahmadi. Polyploidy and comparison of diploid and autotetraploid seedling of Madagascar periwinkle (Catharanthus roseus cv. alba). Intl. Res. J. Appl. Basic. 2013; 4, 402-6.

H Wu, S Zheng, Y He, G Yan, Y Bi and Y Zhu. Diploid female gametes induced by colchicine in Oriental lilies. Scientia Horticulturae 2007; 114, 50-3.

RJ Griesbach. Colchicine-induced polyploidy in Phalaenopsis orchids. Plant Cell Tiss. Organ Cult. 1981; 1, 103-7.

P Atichart. Polyploid induction by colchicine treatments and plant regeneration of Dendrobium chrysotoxum. Thai J. Agri. Sci. 2013; 46, 59-63.

S Sarathum, M Hegele, S Tantiviwat and M Nanakorn. Effect of concentration and duration of colchicine treatment on polyploidy induction in Dendrobium scabrilingue L. Europ. J. Hort Sci. 2010; 75, 123-7.

P Atichart and S Bunnag. Polyploid induction in Dendrobium secundum (Bl.) Lindl. by in vitro techniques. Thai J. Agri. Sci. 2007; 40, 91-5.

N Kerdsuwan and S Te-chato. Effect of colchicine on survival rate, morphological, physiological and cytological characteristics of chang daeng orchid (Rhynchostylis gigantean var. rubrum Sagrik) in vitro. J. Agri. Tech. 2012; 8, 1451-60.

MY Chung, CY Kim, JS Min, DJ Lee, AH Naing, JD Chung and CK Kim. In vitro induction of tetraploids in an interspecific hybrid of Calanthe (Calanthe discolor × Calanthe sieboldii) through colchicine and oryzalin treatments. Plant Biotech. Rep. 2014; 8, 251-7.

NTP Thao, K Ureshino, I Miyajima, Y Ozaki and H Okubo. Induction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments. Plant Cell Tissue Organ Cult. 2003; 72, 19-25.

J Doležel and W Göhde. Sex determination in Dioecious plants Melandrium album and M. rubrum using high-resolution flow cytometry. Cytometry 1995; 19, 103-6.

T Murashige and F Skoog. A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol. Plantarum 1962; 15, 473-97.

W Pornchuti and K Thammasiri. Effects of BA and NAA on micropropagation of Spathoglottis plicata Blume. In: Proceedings of the 34th Congress on Science and Technology of Thailand, Bangkok, Thailand, 2008.

Downloads

Published

2015-11-06

How to Cite

PORNCHUTI, W., THAMMASIRI, K., CHUENBOONNGARM, N., & PANVISAVAS, N. (2015). Alteration of Spathoglottis eburnea Gagnep. Ploidy Level after Colchicine Treatments. Walailak Journal of Science and Technology (WJST), 14(3), 243–252. Retrieved from https://wjst.wu.ac.th/index.php/wjst/article/view/1692

Most read articles by the same author(s)