Plantlet Regeneration and Multiple Shoot Induction from Protocorm-Like Bodies (PLBs) of Medicinal Orchid Species, Dendrobium crumenatum Sw.

Sutha  KLAOCHEED; Suphat  RITTIRAT; Kanchit  THAMMASIRI

doi:10.48048/wjst.2021.9168

Authors

Sutha KLAOCHEED Department of Technology and Industries, Faculty of Science and Technology, Prince of Songkla University, Pattani campus, Pattani 94000, Thailand
SUPHAT RITTIRAT Faculty of Science and Technology, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280, Thailand
Kanchit THAMMASIRI Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

DOI:

https://doi.org/10.48048/wjst.2021.9168

Keywords:

Dendrobium crumenatum, Germplasm conservation, In vitro conservation, Micropropagation, Thidiazuron

Abstract

To investigate the suitable medium for in vitro shoot regeneration and plantlet growth of Dendrobium crumenatum Sw., individual protocorm-like bodies (PLBs) (about 4 - 5 mm in diameter) of Dendrobium crumenatum Sw. derived from MS medium supplemented with 0.5 mg/L TDZ for 60 days of culture were cultured on 6 culture media; Murashige and Skoog (MS) medium, MS medium supplemented with 15 % (v/v) CW, MS medium supplemented with 15 % (v/v) CW and 0.2 % (w/v) AC, Vacin and Went (VW) medium, VW medium supplemented with 15 % (v/v) CW, VW medium supplemented with 15 % (v/v) CW and 0.2 % (w/v) AC. After 4 months of culture, MS medium containing 15 % coconut water (CW) gave the highest percentage of shooting and number of shoots per explant of 96.0 and 9.5, respectively with a significant difference from other media. The addition of 0.2 % (w/v) activated charcoal (AC) significantly increased the number of leaves and roots. PLBs developed into complete plantlets. MS medium supplemented with 15 % (v/v) CW and 0.2 % (w/v) AC and VW medium supplemented with 15 % (v/v) CW and 0.2 % (w/v) AC gave the highest number of roots per plantlet and root length at 5.3 roots and 34.9 mm, respectively. After the transfer of rooted shoots to the greenhouse, 95 % of the regenerated plantlets survived and grew vigorously. Plantlets grown in vitro were successfully acclimatized in the greenhouse and showed normal development.

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