Cryopreservation of Dendrobium cruentum Rchb. f. Seeds by D Cryo-plate and V Cryo-plate Techniques

Authors

  • Sasikarn PRASONGSOM Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
  • Kanchit THAMMASIRI Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
  • Jarunya NARANGAJAVANA Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
  • Siripong THITAMADEE Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
  • Ngarmnij CHUENBOONNGARM Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
  • Nathinee PANVISAVAS Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

DOI:

https://doi.org/10.48048/wjst.2020.6159

Keywords:

Orchid, Dendrobium cruentum Rchb. f. seeds, cryopreservation, D cryo-plate, V cryo-plate

Abstract

Dendrobium cruentum Rchb. f. is a native Thai orchid species that has faced extinction because of its attractive characteristics. Consequently, conservation of this species is urgently needed. In this study, cryopreservation technique was applied to D. cruentum Rchb. f. seeds for long-term conservation. A successful protocol for D. cruentum Rchb. f. seed cryopreservation was developed by using D cryo-plate and V cryo-plate techniques. Seed viability was tested by TCC solution and 93.8 % of dyed seeds were shown. For cryo-plate technique, seeds were encapsulated over the cryo-plate by using 2 % (w/v) sodium alginate and polymerized with 100 mM CaCl2. Encapsulated seeds were desiccated by using a laminar airflow and PVS2 solution treatment with the same exposure time (0, 30, 60, 90, and 120 min). After cryopreservation, encapsulated seeds were cultured on modified VW agar medium. From the results, the maximum germination and regrowth percentage were observed; D cryo-plate technique with 60 min of dehydration time gave the highest germination (68.9 %) and regrowth (57.8 %). Thus, the excess of dehydration may cause the reduction of germination and plant regeneration. In conclusion, D cryo-plate technique proved to be appropriate for D. cruentum Rchb. f. seed cryopreservation.

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Published

2019-04-04

How to Cite

PRASONGSOM, S., THAMMASIRI, K., NARANGAJAVANA, J., THITAMADEE, S., CHUENBOONNGARM, N., & PANVISAVAS, N. (2019). Cryopreservation of Dendrobium cruentum Rchb. f. Seeds by D Cryo-plate and V Cryo-plate Techniques. Walailak Journal of Science and Technology (WJST), 17(3), 181–191. https://doi.org/10.48048/wjst.2020.6159

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