Morphological and Stomatal Guard Cell Characteristics of in vitro Kaempferia rotunda L. (Zingiberaceae) through Colchicine Induced Polyploidy

Sutthinut SOONTHORNKALUMP; Ngarmnij CHUENBOONNGARM; Thaya JENJITTIKUL; Kanchit THAMMASIRI; Puangpaka SOONTORNCHAINAKSAENG

Authors

Sutthinut SOONTHORNKALUMP Master of Science Program in Plant Science, Department of Plant Science, Faculty of Science and Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok 10400
Ngarmnij CHUENBOONNGARM Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400
Thaya JENJITTIKUL Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400
Kanchit THAMMASIRI Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400
Puangpaka SOONTORNCHAINAKSAENG Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400

Keywords:

Colchicine, flow cytometry, tetraploid, variegated leaf, plant improvement

Abstract

Kaempferia rotunda L. was introduced to plant improvement programs by polyploidy induction for developing new ornamental gingers. In order to improve some characteristics of K. rotunda, tetraploid plants were induced by in vitro culturing young shoots of K. rotunda on gelrite modified MS medium supplemented with 0.2 % colchicine for 4 days. After incubation, all of the explants were transferred to culture on shoot multiplication medium and subcultured until M₁V₄ generation for chimera segregation. Ploidy determinations were made by flow cytometry analysis. Stomatal guard cell size, leaf thickness, and chloroplast numbers in the stomata of polyploid plants were significantly increased when compared with diploid plants. However, difference of stomatal index was not found in all polyploid plants. Two solid lines of tetraploid plants, normal green and variegated dwarf, were generated. The leaves of the normal green group were thicker and wider than those of diploid plants. In contrast, the variegated dwarf group showed smaller leaves with chlorophyll mutation.

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References

K Larsen, and SS Larsen. Gingers of Thailand. Queen Sirikit Botanic Garden, Chiang Mai, 2006.

BE van Wyk. Food Plant of the World. Briza Publications, South Africa, 2005.

K Sereena, KU Prakash and SAB Rema. Histochemical and phytochemical markers for the authentication of ayurvedic raw drug hallakam (Kaempferia rotunda) and its marketed adulterant. Int. J. Pharmaceut. Sci. Res. 2011; 2, 2952-8.

HJ Woerdenbag, T Windono, R Bos, S Riswan and WJ Quax. Composition of the essential oils of Kaempferia rotunda L. and Kaempferia angustifolia Roscoe rhizomes from Indonesia. Flavour Fragrance J. 2004; 19, 145-8.

PD Lotulung, Minarti, LB Kardono and K Kawanishi. Antioxidant compound from the rhizomes of Kaempferia rotunda L. Pakistan J. Biol. Sci. 2008; 11, 2447-50.

G Nuki. Colchicine: Its mechanism of action and efficacy in crystal-induced inflammation. Curr. Rheumatol. Rep. 2008; 10, 218-27.

H Wollerson, F Erdmann, R Dettemeyer and M Risse. Accidental fatal ingestion of colchicine-containing leaves - toxicological and histological findings. Leg. Med. 2009; 11, S498-S499.

A Nasreen, H Gundlach and MH Zenk. Incorporation of phenethylisoquinolines into colchicine in isolated seeds of Colchicum autumnale. Phytochemistry 1997; 46, 107-15.

A Poutaraud and P Girardin. Agronomical and chemical variability of Colchicum autumnale accessions. Can. J. Plant Sci. 2006; 86, 547-55.

J Ramsey and DW Schemske. Pathways, mechanisms, and rates of polyploidy formation in flowering plants. Annu. Rev. Ecol. Systemat. 1998; 29, 467-501.

A Omran and B Mohammad. B. Polyploidization effect in two diploid cotton (Gossypium herbaceum L. and G. arboreum L.) species by colchicine treatments. Afr. J. Biotechnol. 2008; 7, 102-8.

AS Escandón, LM Alderete and JC Hagiwara. In vitro polyploidization of Mecardonia tenella, a native plant from South America. Sci. Hort. 2007; 115, 56-61.

L Chen,Y Wang and M Zhoa. In vitro and characterization of tetraploid Lychnis senno Siebold et Zucc. HortScience 2006; 41, 759-61.

C Chen, X Hou, H Zhang, G Wang and L Tian. Induction of Anthurium andraeanum ‘‘Arizona’’ tetraploid by colchicine in vitro. Euphytica 2011; 18, 137-45.

S Gantait, N Mandal, S Bhattacharyya and PK Das. Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella. Plant Cell Tissue Organ Cult. 2011; 106, 485-93.

HF Sakhanokho, KR Rowena, RY Kelley and N Islam-Faridi. Induced polyploidy in diploid ornamental ginger (Hedychium muluense R. M. Smith) using colchicine and oryzalin. HortScience 2009; 44, 1809-14.

S Adaniya and D Shirai. In vitro induction of tetraploid ginger (Zingiber officinale Roscoe) and its pollen fertility and germinability. Sci. Hort. 2001; 88, 277-87.

T Murashige and F Skoog. A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plantarum 1962; 15, 473-97.

S Soonthornkalump, N Chuenboonngarm, P Soontornchainaksaeng, T Jenjittikul and K Thammasiri. Effect of colchicine incubation time on tetraploid induction of Kaempferia rotunda. In: Proceedings of the International Symposium on Orchids and Ornamental Plants, Chiang Mai, Thailand, 2014, p. 89-92.

J Doležel and W Godhe. Sex determination in dioecious plants Melandrium album and M. rubrum using high-resolution flow cytometry. Cytometry 1995; 19, 103-6.

DL Royer. Stomatal density and stomatal index as indicators of paleoatmospheric CO2 concentration. Rev. Paleaeobot. Palynol. 2001; 114, 1-28.

DH Voss. Relating colorimeter measurement of plant color to the royal horticultural society colour chart. HortScience 1992; 27, 1256-60.

GM Pietsch and NO Anderson. Efficacy colchicine and trifluralin in creating in vitro autotetraploid Gaura lindheimeri Engelm. and Gray. HortScience 2006; 41, 1656-61.

Z Zhang, H Dai, M Xiao and X Liu. In vitro induction of tetraploids in Phlox subulata L. Euphytica 2008; 159, 59-65.

T Kaensaksiri, P Soontornchainaksaeng, N Soonthornchareonnon and S Prathanturarug. In vitro induction of polyploidy in Centella asiatica (L.) Urban. Plant Cell Tissue Organ Cult. 2011; 107, 187-94.

MJ Jaskani, SW Kwon and DH Kin. Flow cytometry of DNA contents of colchicine treated watermelon as a ploidy screening method at M1 stage. Pakistan J. Bot. 2005; 37, 685-96.

D Ewald, K Ulrich, G Naujoks and MB Schröder. Induction of tetraploid poplar and black locust plants using colchicine: Chloroplast number as an early marker for selecting polyploids in vitro. Plant Cell Tissue Organ Cult. 2009; 99, 353-7.

L Wei, H Dong-nan, L Hui and C Xiao-yang. Polyploid induction of Lespedeza formosa by colchicine treatment. Forest. Stud. China 2007; 9, 283-6.

KK Petersen, P Hagberg and K Kristiansen. In vitro chromosome doubling of Miscanthus sinensis. Plant Breed. 2002; 121, 445-50.

AS Islam, K Njorogea and A Irudayanathan. Random chromosome breakage by colchicine in Viscum fischeri (Loranthaceae). Experientia 1977; 34, 328-9.

AM Castillo, L Cistué, MP Vallés and M Soriano. Chromosome Doubling in Monocots. In: A Touraev, BP Forster and SJ Mohan (eds.). Advances in Haploid Production in Higher Plants. Springer, Heidelberg, 2009, p. 329-38.

RJ Griesbach and RN Bhat. Colchicine-induced polyploidy in Eustoma grandiflorum. HortScience 1990; 25, 1284-6.