Morphological and Stomatal Guard Cell Characteristics of in vitro Kaempferia rotunda L. (Zingiberaceae) through Colchicine Induced Polyploidy


  • Sutthinut SOONTHORNKALUMP Master of Science Program in Plant Science, Department of Plant Science, Faculty of Science and Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok 10400
  • Ngarmnij CHUENBOONNGARM Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400
  • Thaya JENJITTIKUL Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400
  • Kanchit THAMMASIRI Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400
  • Puangpaka SOONTORNCHAINAKSAENG Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400


Colchicine, flow cytometry, tetraploid, variegated leaf, plant improvement


Kaempferia rotunda L. was introduced to plant improvement programs by polyploidy induction for developing new ornamental gingers. In order to improve some characteristics of K. rotunda, tetraploid plants were induced by in vitro culturing young shoots of K. rotunda on gelrite modified MS medium supplemented with 0.2 % colchicine for 4 days. After incubation, all of the explants were transferred to culture on shoot multiplication medium and subcultured until M1V4 generation for chimera segregation. Ploidy determinations were made by flow cytometry analysis. Stomatal guard cell size, leaf thickness, and chloroplast numbers in the stomata of polyploid plants were significantly increased when compared with diploid plants. However, difference of stomatal index was not found in all polyploid plants. Two solid lines of tetraploid plants, normal green and variegated dwarf, were generated. The leaves of the normal green group were thicker and wider than those of diploid plants. In contrast, the variegated dwarf group showed smaller leaves with chlorophyll mutation.


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How to Cite

SOONTHORNKALUMP, S., CHUENBOONNGARM, N., JENJITTIKUL, T., THAMMASIRI, K., & SOONTORNCHAINAKSAENG, P. (2016). Morphological and Stomatal Guard Cell Characteristics of in vitro Kaempferia rotunda L. (Zingiberaceae) through Colchicine Induced Polyploidy. Walailak Journal of Science and Technology (WJST), 14(3), 235–242. Retrieved from

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