Investigation of Anticancer Activity of Lindernia crustacea (L.) F. Muell. var. Crustacean

Sahapat BARUSRUX, Natthida WEERAPREEYAKUL, Preeyaporn Plaimee PHIBOONCHAIYANAN, Munthipha KHAMPHIO, Waraporn TANTHANUCH, Kanjana THUMMANU

Abstract


Lindernia crustacea (L.) F. Muell. var. crustacean or “Ya Kap Hoi: YKH” was misunderstood as “Ya Yad Nam Kang” and cancer curative. This study aimed to investigate anticancer activity by the analysis of chemical constituent in extracts and in vitro screening of biological activities of the extract in several aspects such as cancer cell lines cytotoxic activity, immune cell proliferating activity, reducing power activity, alkylation activity. The HPLC analysis showed the absence of plumbagin peak observed in the HPLC chromatograms of both YKH aqueous and YKH ethanolic extracts. The YKH ethanolic extract yielded more chemical constituents than that of the YKH aqueous extract. The YKH aqueous extract was inactive against all cancer cells tested. Interestingly, YKH ethanolic extract caused cancer cell death in HCT116 colon cancer, HepG2 liver cancer, and Jurkat leukemic cancer cell lines in the concentration dependent manner. The following IC50 concentrations of the YKH ethanolic extract that possessed 50 % cell death after 24 h exposure in HCT116, HepG2, and Jurkat cell line were 195.4 ± 12, 171.7 ± 8.7, and 48.8 ± 5.7 µg/mL, respectively. Both aqueous and ethanolic extracts of YKH showed high antioxidant activity based on reducing power activity but did not have alkylation activity. At high concentration (250 µg/mL), YKH ethanolic extract can inhibit immune cells proliferation activity more than the YKH aqueous extract. An unexpected but critical outcome of our studies was the finding that anticancer activity is promised by selecting the plant extraction solvent with less polarity. Potential anticancer constituents were extracted from YKH using ethanol and these constituents cannot be found in aqueous solution of YKH.

Keywords


Alkylation, cell proliferating, cytotoxic, Lindernia crustacean, reducing power, Ya Kap Hoi

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References


Dailynews, Available at: http://www.dailynews.co.th/politics/17058, accessed March 2018.

Web of Department for Development of Thai Traditional and Alternative Medicine, Ministry of Public Health, Available at: http://www.dtam.moph.go.th/internet/admin/spaw2/uploads/files/ dtam16-03-55.pdf, accessed March 2018.

The Encyclopedia of Plants in Thailand, Available at: http://www.dnp.go.th/botany/ detail.aspx?words, accessed March 2018.

T Juengwatanatrakul, S Sakamoto, H Tanaka and W Putalun. Elicitation effect on production of plumbagin in in vitro culture of Drosera indica L. J. Med. Plants. Res. 2011; 5, 4949-53.

R Asirvatham and AJM Christina. Drosera indica L: Potential effect on liver enzyme, lipid profile and hormone change in Dalton’s lymphoma ascites (DLA) bearing mice. J. Intercult. Ethnopharmacol. 2012; 1, 69-73.

R Asirvatham and AJM Christina. Anticancer activity of Drosera indica L. on dalton’s lymphoma ascites (DLA) bearing mice. J. Intercult. Ethnopharmacol. 2013; 2, 9-14.

R Asirvatham, AJM Christina and A Murali. In vitro antioxidant and anticancer activity studies on Drosera indica L. (Droseraceae). Adv. Pharm. Bull. 2013; 3, 115-20.

S Machana, N Weerapreeyakul and S Barusrux. Anticancer effect of the extracts from Polyalthia evecta against human hepatoma cell line (HepG2). Asian Pac. J. Trop. Biomed. 2012; 2, 368-74.

S Machana, N Weerapreeyakul, S Barusrux, A Nonpunya, B Sripanidkulchai and T Thitimetharoch. Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line. Chin. Med. UK 2011; 6, 39.

S Machana, N Weerapreeyakul, S Barusrux, K Thumanu and W Tanthanuch. FTIR microspectroscopy discriminates anticancer action on human leukemic cells by extracts of Pinus kesiya; Cratoxylum formosum ssp. pruniflorum and melphalan. Talanta 2012; 93, 371-82.

P Plaimee, N Weerapreeyakul, S Barusrux and NP Johns. Immunomodulatory effect of melatonin in SK-LU-1 human lung adenocarcinoma cells co-cultured with peripheral blood mononuclear cells. Cell. Prolif. 2014; 47, 406-15.

AJ Potter and MH Wener. Flow cytometric analysis of fluorescence in situ hybridization with dye dilution and DNA staining (flow-FISH-DDD) to determine telomere length dynamics in proliferating cells. Cytometry A 2005; 68, 53-8.

A Rohman, S Riyanto, N Yuniarti, WR Saputra, R Utami and W Mulatsih. Antioxidant activity, total phenolic, and total flavaonoid of extracts and fractions of red fruit (Pandanus conoideus Lam). Int. Food Res. J. 2010; 17, 97-106.

D Thomas, R Barbey, D Henry and Y Surdin-Kerjan. Physiological analysis of mutants of Saccharomyces cerevisiae impaired in sulphate assimilation. J. Gen. Microbiol. 1992; 138, 2021-8.

P Prayong, S Barusrux and N Weerapreeyakul. Cytotoxic activity screening of some indigenous Thai plants. Fitoterapia 2008; 79, 598-601.

S Machana, N Weerapreeyakul, S Barusrux, K Thumanu and W Tanthanuch. Synergistic anticancer effect of the extracts from Polyalthia evecta caused apoptosis in human hepatoma (HepG2) cells. Asian Pac. J. Trop. Biomed. 2012; 2, 589-96.

M Khamphio, S Barusrux and N Weerapreeyakul. Sesamol induces mitochondrial apoptosis pathway in HCT116 human colon cancer cells via pro-oxidant effect. Life Sci. 2016; 158, 46-56.

S Siriamornpun, N Weerapreeyakul and S Barusrux. Bioactive compounds and health implications are better for green jujube fruit than for ripe fruit. J. Func. Food. 2015; 12, 246-55.

Melphalan. National Cancer Institute, Available at: https://www.cancer.gov/publications/ dictionaries/cancer-drug/def/melphalan-hydrochloride, accessed August 2014.

N Weerapreeyakul, S Machana and S Barusrux. Synergistic effects of melphalan and Pinus kesiya Royle ex Gordon (Simaosong) extracts on apoptosis induction in human cancer cells. Chin Med. UK 2016; 11, 29.

GK McEvoy. AHFS Drug Information. American Society of Health-System Pharmacists, Bethesda, 2006, p. 1131-5.

BD Rose. Melphalan: Pediatric Drug Information, Waltham, Massachusetts: UpToDate 14.3, Available at: www.uptodate.com, accessed November 2006.

N Taechakulwanijya, N Weerapreeyakul, S Barusrux and S Siriamornpun. Apoptosis-inducing effects of jujube (Zǎo) seed extracts on human Jurkat leukemia T cells. Chin. Med. UK 2016; 11, 15.

N Weerapreeyakul, A Nonpunya, S Barusrux, T Thitimetharoch and B Sripanidkulchai. Evaluation of the anticancer potential of six herbs against a hepatoma cell line. Chin. Med. UK 2012; 7, 15.

N Weerapreeyakul, J Cholpajsorn, S Barusrux and T Thitimetharoch. Induction of apoptosis in human hepatocellular carcinoma cells by extracts of Lannea coromandelica (Houtt.) Merr. and Diospyros castanea (Craib) Fletcher. Chin. Med. UK 2016; 11, 19.


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