Anticancer Activity of Lindernia crustacea (L.) F. Muell. var. Crustacean on Human HCT116 Colon Cancer Cell via Cellular Lipid and β-sheet Protein Accumulation

Sahapat  BARUSRUX; Natthida  WEERAPREEYAKUL; Preeyaporn Plaimee  PHIBOONCHAIYANAN; Munthipha  KHAMPHIO; Waraporn  TANTHANUCH; Kanjana  THUMMANU

doi:10.48048/wjst.2020.10718

Authors

Sahapat BARUSRUX Research of Excellence Center for Innovative Health Products (RECIHP), School of Allied Health Science, Walailak University, Nakhon Si Thammarat 80160, Thailand
Natthida WEERAPREEYAKUL Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
Preeyaporn Plaimee PHIBOONCHAIYANAN Faculty of Pharmaceutical Sciences, Rangsit University, Pathum Thani 12000, Thailand
Munthipha KHAMPHIO Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
Waraporn TANTHANUCH Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima 30000, Thailand
Kanjana THUMMANU Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima 30000, Thailand

DOI:

https://doi.org/10.48048/wjst.2020.10718

Keywords:

Lindernia crustacean, Ya Kap Hoi, Anticancer, FTIR

Abstract

Lindernia crustacea (L.) F. Muell. var. crustacean or “Ya Kap Hoi: YKH”, which is an edible vegetable, is commonly seen in Thailand. This study investigated its anticancer properties with high antioxidant activity by reducing power activity, excluding alkylation activity. It explored YKH extract induction of anticancer activity through biomolecular changes in the HCT116 human colon cancer cell line. The ethanolic extract stock solution and water extract stock solution were prepared. NR assay was used for cancer cell cytotoxic testing and Fourier transform infrared (FTIR) microspectroscopy was used for biomolecular changes study on lipid, protein and nucleic acid/DNA. The apoptosis induction by the extracts was detected by using Annexin V-FITC and DAPI staining. The compounds in the YKH ethanolic extract was performed by using GC-MS analysis. As a result, YKH ethanolic extract caused HCT116 colon cancer cell death in the dose dependent manner after 24 h exposure, and the 50 % cell death (IC₅₀ concentration) was 195.4 ± 12 μg/mL. Cellular biochemical changes observed from FTIR data showed that YKH ethanolic extract treated HCT116 colon cancer cell. There is an increase in lipid content and a reduction in intensity of nucleic acid/DNA, a-helix protein structure at 1,656 cm^-1was reduced and peak position of b-sheet structure (1,637 cm^-1) was shifted to lower frequency. From the analysis results, YKH ethanolic extract seems to exert anti-colon cancer effect by changing cellular biomolecular structure of lipids and β-sheet protein accumulation, supporting apoptotic induction. The compounds in the YKH ethanolic extract mainly yielded fatty acids,which may be useful as potential compounds.

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