cDNA Cloning and Expression of Translationally Controlled Tumour Protein (TCTP) Isolated from Mud Crab Scylla paramamosain in Escherichia coli

Phuwadol BANGRAK

Abstract


Translationally controlled tumour proteins (TCTP) cDNA were cloned from the haemolymph of Scylla paramamosain by reverse transcription PCR using primers derived from Penaeus monodon. Nucleotide sequence analysis revealed 507 bp open reading frame (ORF) encoding 168 amino acid residues with a predicted molecular mass and pI of approximately 19.2 kDa and 4.5, respectively. Interestingly, mud crab-TCTP shows 99 % homology with Penaeus monodon translationally controlled tumour proteins (Pm-TCTP) and Fenneropenaeus merguiensis TCTP. This gene was also expressed in pQE40 vector as histidine tagged fusion proteins. The recombinant protein has a molecular mass of approximately 25 kDa. From the biological function study of Pm-TCTP, It is proposed that mud crab-TCTP might function as anti-apoptosis like Pm-TCTP does.


Keywords


Scylla paramamosain, TCTP, biodefense molecule, RT-PCR

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H Bohm, R Bendorf, M Gaestel, B Gross, P Nurnberg, R Kraft, A Otto and H Bielka. The growth related protein p23 of the ehrlich ascites tumour: translational control, cloning and primary structure. Biochem. Int. 1989; 19, 277-86.

R Yenofski, S Cereghini, A Krowcynska and G Brawerman. Regulation of mRNA utilization in mouse erythroleukemia cells induced to differentiate by exposure dimethyl sulfoxide. Mol. Cell Biol. 1983; 3, 1197-203.

ST Chitpatima, S Makrides, R Banyopadhyay and G Brawerman. Nucleotide sequence of a major messenger RNA for a 21 kilodalton peptide that is under translational control in mouse tumour cells. Nucleic Acids Res. 1998; 16, 2350-55.

B Gross, M Gaestel, H Bohm, H Bielka. cDNA sequence coding for a translationally controlled human tumour protein. Nucleic Acids Res. 1989; 17, 8367.

A Pay, E Heberle-Bors and H Hirt. An alfalfa cDNA encodes a protein with homology to translationally controlled human tumour protein. Plant Mol. Biol. 1992; 19, 501-3.

J Bhisutthibhan, XQ Pan, PA Hossler, DJ Walker, CA Yowell, J Carlton, JB Dame and SR Meshnick. The Plasmodium falciparum translationally controlled tumour protein homolog and its reaction with the antimalarial drug artemisinin. J. Biol. Chem. 1998; 273, 16192-198.

SR Sturzenbaum, P Kille and AJ Morgan. Identification of heavy metal induced changes in the expression patterns of the translationally controlled tumour protein (TCTP) in the earthworm Lumbricus rubellus1. Biochim. Biophys. Acta 1998; 1398, 294-304.

Y Gachet, S Tournier, M Lee, A Lazaris-Karatzas, T Poulton and UA Bommer. The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle. J. Cell Sci. 1999; 112, 1257-71.

M Kim, Y Jung, K Lee and C Kim. Identification of the calcium binding sites in translationally controlled tumor protein. Arch. Pharm. Res. 2000; 23, 633-6.

CH Niak, KW Su and RC Ko. Identification of some heat-induced genes of Trichinella spiralis. Parasitology 2001; 123(Pt3), 293-300.

IK Budde, M Aalbers, RC Aalberse, JS van der Zee and EF Knol. Reactivity to IgE-dependent histamine-releasing factor is due to monomeric IgE. Allergy. 2000; 55, 653-7.

SM Macdonald, T Rafnar, J Langdon and LM Lichtenstein. Molecular identification of an IgE-dependent histamine releasing factor. Science 1995; 269, 688-90.

F Li, D Zhang and K Fujise. Characterization of fortilin, a novel antiapoptotic protein. J. Biol. Chem. 2001; 276, 47542-549.

D Zhang, F Li, D Weidner, ZH Mnjoyan and K Fujise. Physical and functional interaction between myeloid cell leukemia 1 protein (MCL1) and fortilin. J. Biol. Chem. 2002; 277, 37430-438.

P Bangrak, P Graidist, W Chotigeat and A Phongdara. Molecular cloning and expression of a mammalian homologue of a translationally controlled tumor protein (TCTP) gene from Penaeus monodon shrimp. J. Biotech. 2004; 108, 219-26.

P Graidist, K Fujise, W Wanna, K Sritunyalucksana and A Phongdara. Establishing a role for shrimp fortilin in preventing cell death. Aquaculture 2006; 255, 157-64.

P Thaw, NJ Baxter, AM Hounslow, C Prince, JP Waltho and CJ Craven. Structure of TCTP reveals unexpected relationship with guanine nucleotide free chaperones. Nature Structural Biol. 2001; 8, 701-4.

T Venugopal. Evolution and expression of translationally controlled tumour protein (TCTP) of fish. Comp. Biochem. Physiol. Part B 2005; 142, 8-17.

M Gnanasekar, KVN Rao, L Chen, RB Narayanan, M Greetha, AL Scott, K Ramasma and P Kaliraj. Molecular characterization of a calcium binding translationally controlled tumour protein homologue from the filarial parasites Brugia malayi and Wuchereria bancrofti. Mol. Biochem. Parasitol. 2002; 121, 107-18.

UA Bommer and BJ Thiele. The translationally controlled tumour protein (TCTP). Int. J. Biochem. Cell Biol. 2004; 36, 379-85.


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