Synthesis, Characterization and Antitumor Efficacy of Silver Nanoparticle from Agaricus bisporus Pileus, Basidiomycota
Keywords:AFM, anticancer activity, EDS, nanomedicine, ultra violet, XRD
The object of this study is to synthesize and characterize silver nanoparticles from Agaricus bisporus pileus extracts and their applications. Agaricus bisporus-mediated synthesis of AgNPs was characterized using changing the color solution, UV-Visible spectroscopy, SEM, AFM, SPM, FTIR spectrum, XRD, and EDS analyses. The change of the mixture color of 10-3 M AgNO3 with the watery extract of fresh A. bisporus caps from colorless to brown color is an indicator for the formation of silver nanoparticles (AgNPs). The UV-Visible spectrum exhibits the absorption peak at 418 nm. The FTIR spectra exhibited that the structures of amino acids, polysaccharides, and polyphenols in the crude extract of A. bisporus are not affected because of the joining and interaction of their functional groups with silver ions, and act as reducing and capping agents to the biosynthesized Ag nanoparticles. SEM and EDS refer to the formation of AgNPs with irregular or spherical shapes. The XRD pattern exhibits face-centered cubic (fcc) silver nanocrystals, with crystalline AgNPs size of 43.9 nm. The biosynthesized AgNPs play a suitable role against mouse cell line, which has receptors for polioviruses (L20B). After exposure of the colloid AgNPs to UV radiation (256 nm), the absorption band transferred from 418 nm to 435 nm, indicating that UV rays affect on physical properties of AgNPs. Roughness average of the biosynthesized AgNPs from A. bisporus caps is 15.4 nm, but the roughness is increased after UV irradiation for 1 h to average 33.6 nm. Histograms of particle size distribution of AgNPs show the average of AgNPs is 103.57 nm, while the size of nanoparticles reaches 69.47 nm after exposure to UV radiation of 256 nm. The use of UV radiation leads to enhanced characteristics of silver nanoparticles.
T Vo-Dinh. Protein Nanotechnology, Protocols, Instrumentation, and Applications. Humana Press, Totowa, NJ, 2005. p. 1-7.
A Gade, A Ingle, C Whiteley and M Rai. Mycogenic metal nanoparticles: Progress and applications. Biotechnol. Lett. 2010; 32, 593-600.
KW Lem, A Choudhury, AA Lakhani, P Kuyate, JR Haw, DS Lee, Z Iqbal and CJ Brumlik. Use of nanosilver in consumer products. Recent Pat. Nanotechnol. 2012; 6, 60-72.
SK Srikar, DD Giri, DB Pal, PK Mishra and SN Upadhyay. Green synthesis of silver nanoparticles: A review. Green Sustain. Chem. 2016; 6, 34-56.
S Farhadi, B Ajerloo and A Mohammadi. Green biosynthesis of spherical silver nanoparticles by using date palm (Phoenix dactylifera) fruit extract and study of their antibacterial and catalytic activities. Acta Chim. Slov. 2017; 64, 129-43.
B Kumar, L Cumbal and A Debut. Phycosynthesis of silver nanoparticles using Calothrix algae through ultrasonic method. In: Proceedings of the XI Congreso de Ciencia Y Tecnologia ESPE. Sangolqui, Ecuador, 2016. p. 213-6.
AM Abdel-Hadi, MF Awad, NF Abo-Dahab and MF ElKady. Extracellular synthesis of silver nanoparticles by aspergillus terreus: Biosynthesis, characterization and biological activity. Biosci. Biotechnol. Res. Asia 2015; 11, 1179-86.
C Malarkodi, S Rajeshkumar, K Paulkumar, G Gnanajobitha, M Vanaja and G Annadurai. Bacterial synthesis of silver nanoparticles by using optimized biomass growth of Bacillus sp. Nanosci. Nanotechnol. An. Int. J. 2013; 3, 26-32.
A Jaganathan, K Murugan, C Panneerselvam, P Madhiyazhagan, D Dinesh, C Vadivalagan, A Thabiani, B Chandramohan, U Suresh, R Rajaganesh, J Subramaniam, M Nicoletti, A Higuchi, AA Alarfaj, MA Munusamy and S Kumar. Parasitology international earthworm-mediated synthesis of silver nanoparticles: A potent tool against hepatocellular carcinoma, Plasmodium falciparum parasites and malaria mosquitoes. Parasitol. Int. 2016; 65, 276-84.
MN Owaid and IJ Ibraheem. Mycosynthesis of nanoparticles using edible and medicinal mushrooms. Eur. J. Nanomed. 2017; 9, 5-23.
F Atila, MN Owaid and MA Shariati. The nutritional and medical benefits of Agaricus bisporus: A review. J. Microbiol. Biotechnol. Food Sci. 2017; 7, 281-6.
FAO. Food and Agriculture Organization of the United Nations. 2016.
D Rodrigues, AC Freitas, L Pereira, TAP Rocha-Santos, MR Vasconcelos, LM Rodriguez-Alcala, TAP Rocha-Santos, MR Vasconcelos, LM Rodriguez-Alcala, AMP Gomes and AC Duarte. Chemical composition of red, brown and green macroalgae from Buarcos bay in Central West Coast of Portugal. Food Chem. 2015; 183, 197-207.
MN Owaid, A Barish and MA Shariati. Cultivation of Agaricus bisporus (button mushroom) and its usages in the biosynthesis of nanoparticles. Open Agr. 2017; 2, 537-43.
T Sudhakar, A Nanda, SG Babu, S Janani, MD Evans and TK Markose. Synthesis of silver nanoparticles from edible mushroom and its antimicrobial activity against human pathogens. Int. J. PharmTech Res. 2014; 6, 1718-23.
EA Loshchinina, EP Vetchinkina, MA Kupryashina, VF Kursky and VE Nikitina. Nanoparticles synthesis by Agaricus soil basidiomycetes. J. Biosci. Bioeng. 2018; 126, 44-52.
US Senapati, DK Jha and D Sarkar. Structural, optical, thermal and electrical properties of fungus guided biosynthesized zinc sulphide nanoparticles. Res. J. Chem. Sci. 2015; 5, 33-40.
MN Owaid, J Raman, H Lakshmanan, SSS Al-Saeedi, V Sabaratnam and AI Ali. Mycosynthesis of silver nanoparticles by Pleurotus cornucopiae var. citrinopileatus and its inhibitory effects against Candida sp. Mater. Lett. 2015; 153, 186-90.
PL Chih, JT Wei, LL Yuang and CK Yuh. The extracts from nelumbonucifera suppress cell cycle progression, cytokine genes expression, and cell proliferation in human peripheral blood mononuclear cells. Life Sci. 2004; 75, 699-716.
RI Freshney. Culture of Animal Cell. 6th eds. Wily-Liss, New York, 2012.
SS Shankar, A Ahmad and M Sastry. Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnol. Prog. 2003; 19, 1627-31.
EG Goh, X Xu and PG McCormick. Effect of particle size on the UV absorbance of zinc oxide nanoparticles. Scr. Mater. 2014; 78-79, 49-52.
RP Chahal, S Mahendia, AK Tomar and S Kumar. Effect of ultraviolet irradiation on the optical and structural characteristics of in-situ prepared PVP-Ag. Dig. J. Nanomater. Biostruct. 2011; 6, 299-306.
J Zheng, JD Clogston, AK Patri, MA Dobrovolskaia and SE McNeil. Sterilization of silver nanoparticles using standard gamma irradiation procedure affects particle integrity and biocompatibility. J. Nanomed. Nanotechnol. 2011; S5, 1-6.
R Silverstein, F Webster and D Kiemle. Spectrometric Identification of Organic Compounds. 7th eds. John Wiley and Sons, London, UK, 2005, p. 72-126.
BD Mistry. A Handbook of Spectroscopic Data CHEMISTRY (UV, JR, PMR, JJCNMR and Mass Spectroscopy). 2009th eds. Oxford Book Company, UK, 2009, p. 26-56.
LD Field, S Sternhell and JR Kalman. Organic Structures from Spectra. 4th eds. John Wiley and Sons, UK, 2008, p. 15-20.
K Nakamoto. Infrared and Raman Spectra of Inorganic and Coordination Compounds Part A: Theory and Applications in Inorganic Chemistry. 6th eds. John Wiley and Sons, UK, 2009, p. 1-13.
J Simek. Organic Chemistry. 8th eds. Pearson Education, 2013, p. 412-4.
OH Abid, HM Tawfeeq and RF Muslim. Synthesis and characterization of novel 1,3-oxazepin-5(1H)-one derivatives via reaction of imine compounds with isobenzofuran-1(3H)-one. Acta Pharm. Sci. 2017; 55, 43-55.
S Sujatha, S Tamilselvi, K Subha and A Panneerselvam. Studies on biosynthesis of silver nanoparticles using mushroom and its antibacterial activities. Int. J. Curr. Microbiol. App. Sci. 2013; 2, 605-14.
V Kathiravan, S Ravi and S Ashokkumar. Synthesis of silver nanoparticles from Melia dubia leaf extract and their in vitro anticancer activity. Spectrochim Acta Part A Mol. Biomol. Spectrosc. 2014; 130, 116-21.
HA Abod, I Bander and SS Zain-Al-Abddeen. The effect of silver nanoparticles prepared using Aspergillus niger in some pathogenic bacteria Aspergillus niger. Kirkuk Univ. J. Sci. Stud. 2017; 12, 1-16.
M Ghareib, M Abu, M Mostafa and WE Abdallah. Rapid extracellular biosynthesis of silver nanoparticles by Cunninghamella phaeospora culture supernatant. Iran J. Pharm. Res. 2016; 15, 915-24.
K Abdelrahim, S Younis, A Mohamed, K Salmeen, AEMA Mustafa and S Moussa. Extracellular biosynthesis of silver nanoparticles using Rhizopus stolonifer. Saudi. J. Biol. Sci. 2017; 24, 208-16.
MVDZ Park, AM Neigh and JP Vermeulen. The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles. Biomaterials 2011; 32, 9810-7.
KS Siddiqi, A Husen and RAK Rao. A review on biosynthesis of silver nanoparticles and their biocidal properties. J. Nanobiotechnol. 2018; 16, 14.
S Kummara, MB Patil and T Uriah. Synthesis, characterization, biocompatible and anticancer activity of green and chemically synthesized silver nanoparticles: A comparative study. Biomed. Pharmacother. 2016; 84, 10-21.
HM Abd-Enaby, GM Abo-Elala, UM Abdel-Raouf and MM Hamed. Antibacterial and anticancer activity of extracellular synthesized silver nanoparticles from marine Streptomyces rochei MHM13. Egypt. J. Aquat. Res. 2016; 42, 301-12.
NM El-Deeb, IM El-Sherbiny, MR El-Aassara and EE Hafez. Novel trend in colon cancer therapy using silver nanoparticles synthesized by honey bee. J. Nanomed. Nanotechnol. 2015; 6, 265.
MA Franco-Molina, E Mendoza-Gamboa, CA Sierra-Rivera, RA Gómez-Flores, P Zapata-Benavides, P Castillo-Tello, JM Alcocer-González, DF Miranda-Hernández, RS Tamez-Guerra and C Rodríguez-Padilla. Antitumor activity of colloidal silver on MCF-7 human breast cancer cells. Padilla J. Exp. Clin. Cancer Res. 2010; 29, 148.
MN Owaid, R Muslim and HA Hamad. Mycosynthesis of silver nanoparticles using Terminia sp. desert truffle, pezizaceae, and their antibacterial activity. Jordan J. Biol. Sci. 2018; 11, 401-5.