Cultivation Performance of Pleurotus salmoneostramineus Mushroom on Wastes of Date-palm Trunk, Phoenix dactylifera L., and Woodworking Sawdust

Mustafa Nadhim OWAID, Sajid Salahuddin Saleem AL-SAEEDI, Idham Ali ABED


The objective of this study is to produce Pleurotus salmoneostramineus pink oyster mushroom using organic farming from some surplus local organic wastes, viz., the WSF1 formula (70 % wheat straw, 20 % woodworking sawdust, and 10 % date-palm fiber), the WSF2 formula (50 % wheat straw, 30 % woodworking sawdust, and 20 % date-palm fiber), the S formula (woodworking sawdust), the F formula (fibers of date-palm (Phoenix dactylifera L.) trunk), and the W formula (wheat straw) as a control. The WSF1 formula gave the best yield and biological efficiency (163.2 g/bag and 45.5 %, respectively) with significant differences (p < 0.05), while the S formula test recorded 27.8 g/bags and 6.5 %, respectively. However, F formula showed the highest number, reaching 35 basidiocarps in comparison to the control (wheat straw, 30 basidiocarps), followed by 28 basidiocarps with the WSF1 formula, whereas the S formula showed the lowest number of basidiocarps (6 basidiocarps). However, the WSF1 formula showed the best rate of basidiocarp weight (5.7 g) and diameter of pileus (9.0 cm). Significantly (p < 0.05), the WSF1 formula showed a shorter length (11.9 mm), which was considered to be the best value in comparison with the control (19.5 mm). The S formula exhibited a low diameter of pileus (6.6 cm) and a longer stipe (18.6 mm), respectively. In conclusion, WSF1 is considered to be the best formula for the cultivation of this mushroom.


Myco-degradation, higher basidiomycetes, pink oyster mushroom, biological efficiency, recycling

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DS Hibbett, M Binder, JF Bischoff, M Blackwell, PF Cannon, OE Eriksson, S Huhndorf, T James, PM Kirk, R Lücking, HL Thorsten, F Lutzoni, PB Matheny, DJ McLaughlin, MJ Powell, S Redhead, CL Schoch, JW Spatafora, JA Stalpers, R Vilgalys, MC Aime, A Aptroot, R Bauer, D Begerow, GL Benny, LA Castlebury, PW Crous, YC Dai, W Gams, DM Geiser, GW Griffith, C Gueidan, DL Hawksworth, G Hestmark, K Hosaka, RA Humber, KD Hyde, JE Ironside, U Kõljalg, CP Kurtzman, KH Larsson, R Lichtwardt, J Longcore, J Miadlikowska, A Miller, JM Moncalvo, S Mozley-Standridge, F Oberwinkler, E Parmasto, V Reeb, JD Rogers, C Roux, L Ryvarden, JP Sampaio, A Schüssler, J Sugiyama, RG Thorn, L Tibell, WA Untereiner, C Walker, Z Wang, A Weir, M Weiss, MM White, K Winka, YJ Yao and N Zhang. A higher-level phylogenetic classification of the Fungi. Mycol. Res. 2007; 111, 509-47.

S Miki and K Okano. In vitro digestibility of unsterilized rice and wheat straws cultured with Pleurotus salmoneostramineus. Nihon Chikusan Gakkaiho 2005; 76, 407-14.

M Majeed, MU Khan, MN Owaid, MR Khan, MA Shariati, P Igor and GN Ntsefong. Development of oyster mushroom powder and its effects on physicochemical and rheological properties of Bakery products. J. Microbiol. Biotech. Food Sci. 2017; 6, 1221-7.

KN Yoon, N Alam, MJ Shim and TS Lee. Hypolipidemic and antiatherogenesis effect of culinary-medicinal pink oyster mushroom, Pleurotus salmoneostramineus L. Vass. (higher Basidiomycetes), in hypercholesterolemic rats. Int. J. Med. Mushrooms 2012; 14, 27-36.

K Okano, Y Iida, M Samsuri, B Prasetya, T Usagawa and T Watanabe. Comparison of in vitro digestibility and chemical composition among sugarcane bagasses treated by four white-rot fungi. Anim. Sci. J. 2006; 77, 308-13.

H Smith, S Doyle and R Murphy. Filamentous fungi as a source of natural antioxidants. Food Chem. 2015; 185, 389-97.

MN Owaid, SSS Al-Saeedi and IAA Al-Assaffii. Antifungal activity of cultivated oyster mushrooms on various agro-wastes. Summa Phytopathol. 2017; 43, 9-13.

JH Kim, SJ Kim, HR Park, JI Choi, YC Ju, KC Nam, SJ Kim and SC Lee. The different antioxidant and anticancer activities depending on the color of oyster mushrooms. J. Med. Plants Res. 2009; 3, 1016-20.

DMF Rodrigues, AC Freitas, TAP Rocha-Santos, MW Vasconcelos, M Roriz, LM Rodríguez-Alcalá, AMP Gomes and AC Duarte. Chemical composition and nutritive value of Pleurotus citrinopileatus var cornucopiae, P. eryngii, P. salmoneo stramineus, Pholiota nameko and Hericium erinaceus. J. Food Sci. Tech. 2015; 52, 6927-39.

Y Suzuki, K Okano and T Nakajima. White-rot fungal treatment of unsterilized wheat straw by Pleurotus salmoneo-stramineus to improve its digestibility. Anim. Sci. Tech. 1995; 66, 406-11.

O Myronycheva, I Bandura, N Bisko, AP Gryganskyi and O Karlsson. Assessment of the growth and fruiting of 19 oyster mushroom strains for indoor cultivation on lignocellulosic wastes. BioResources 2017; 12, 4606-26.

AM Hamodi and AM Hameed. Possibility of culturing Mushroom (Agaricus bisporus) in the house garden. J. Kerbala Univ. 2013; 11, 45-53.

YM Al Shamsi. Biological efficiency of recycling organic waste with edible fungi. M.Sc. Thesis, United Arab Emirates University, UAE, 2016, p. 58.

A El-Waziry, F Alkoaik, A Khalil, H Metwally and R Fulleros. Nutrient components and in vitro digestibility of treated and untreated date palm wastes with mushroom (Pleurotus florida). Adv. Anim. Vet. Sci. 2016; 4, 195-9.

F Alkoaik, A Khalil, R Fulleros and RG Reyes. Cultivation of oyster mushroom (Pleurotus florida) on date palm residues in an environmentally controlled conditions in Saudi Arabia. Adv. Environ. Biol. 2015; 9, 955-62.

MH Daneshvar and M Heidari. Effects of wheat straw, leaves of date palm and alfalfa on oyster mushroom yield. In: Proceedings of the 3rd National Congress of Recycling and Reuse of Renewable Organic Resources in Agriculture, 2008.

IA Hassan. Effect of sterilization on the yield and storage life of oyster mushroom cultivated on date palm by products. M.Sc. Thesis, College of Agriculture, University of Baghdad, Iraq, 2011.

AM Kabirifard, H Fazaeli and F Kafilzadeh. Comparing the growth rate of four Pleurotus fungi on wheat stubble and date palm leaf. J. Res. Agric. Sci. 2012; 8, 35-43.

AA Al-Qarawi, EF Abd-Allah and AA Bawadiji. Production of Pleurotus ostreatus on date palm residues. J. Pure Appl. Microbiol. 2013; 7, 1093-7.

KM Alananbeh, NAA Bouqellah and NS Kaff. Cultivation of oyster mushroom Pleurotus ostreatus on date-palm leaves mixed with other agro-wastes in Saudi Arabia. Saudi Arabia Biol. Sci. 2014; 21, 616-25.

MN Owaid, IA Abed and SS Al-Saeedi. Using of date palm fiber mixed with other lignocelluloses toward Pleurotus ostreatus (Higher Basidiomycetes) cultivation. Emirates J. Food Agric. 2015; 27, 556-61.

A Latigui, JM Choi, Z Regagba, DM Allevato and A Latigui. Use of palm (Poenix dctilyfera L.) fiber and sewage sludge co compost as substrates in soilless crop system. Pakistan J. Biol. Sci. 2013; 16, 651-60.

S-T Chang and PG Miles. Mushrooms Cultivation, Nutritional Value, Medicinal Effect and Environmental Impact. 2nd eds. CRC Press LLC. USA, 2004, p. 451.

J Delmas and M Mamoun. Pleurotus cornucopiae, a mushroom which can now be grown in France. PHM Revue Horticole 1983; 240, 39-46.

MN Owaid, AM Abed and BM Nassar. Recycling cardboard wastes to produce blue oyster mushroom Pleurotus ostreatus in Iraq. Emirates J. Food Agric. 2015; 27, 537-41.

RS Al-Juruf. Biological degradation of date-palm fronds used in construction. In: Proceedings of the 6th Saudi Engineering Conference, KFUPM, Dhahran, Arabia Saudi, 2002; 3, 217-22.

MN Owaid, IA Abed and SSS Al-Saeedi. Applicable properties of the bio-fertilizer spent mushroom substrate in organic systems as a byproduct from the cultivation of Pleurotus spp. Inform. Process. Agric. 2017; 4, 78-82.

J Delmas, M Mamoun. First results of the culture of Pleurotus cornucopiae on different substrates. Comptes Rendus des Seances del' Academie d' Agriculture de France 1982; 68, 67-73.

CI Onuoha. Cultivation of the mushroom (Pleurotus tuber regium) using some local substrates. Life Sci. J. 2007; 4, 58-61.

RS Kalpana, AK Mishra and MV Nair. Polymeric products as effective biocide (antifungal agent) against deteriorating wood. Asia. J. Biotech. Resourc. 2011; 2, 542-6.

RA Davis and BJ Aegerter. Edible Mushroom Cultivation. Scientific Publishers Jodhpur India. India, 2000, p. 2-5.

M Ziombra. Influence of substrate and pasteurization on yield of Pleurotus cornucopiae (Paul.:Pers.) roll. J. Veget. Crop. Prod. 2000; 6, 69-73.

M Camassola, LO da Rosa, R Calloni, TA Gaio and AJP Dillon. Secretion of laccase and manganese peroxidase by Pleurotus strains cultivate in solid-state using Pinus spp. sawdust. Brazilian J. Microbiol. 2013; 44, 207-13.

NA Khan, WA Abdul Rehman, M Jahangir and B Khan. Efficiency of oyster mushroom (Pleurotus columbinus - P 8) using date palm leaves with combination of wheat straw and cotton waste for its yield improvement. Pak. J. Bot. 2017; 49, 2459-64.


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