The effects of chitosan mouthrinse on enamel caries in vitro


  • Nattaporn Puangpanboot Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Bangkok 10400
  • Araya Phonghanyudh Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Bangkok 10400
  • Rudee Surarit Department of Oral Biology Faculty of Dentistry, Mahidol University, Bangkok 10400
  • Nuttawee Naimsiri Department of Biotechnology, Faculty of Science, Mahidol University Bangkok 10400


Objective: This study aimed to evaluate the effect of chitosan-containing mouthrinse on de-remineralization process by evaluating surface microhardness (SMH) measurement.

Method: 32 human premolars were cut and embedded in self-cure acrylic resin. After polishing, all samples with window4x4-mm2 were immersed in the demineralizing solution to create artificial caries and then were randomly divided into 4 groups; deionized water as negative control, 0.5% of chitosan, 0.5% of chitosan + 0.05% NaF and 0.05% NaF mouthrinse. They were entered into a 10-day pH cycling twice daily treated with tested mouthrinse. The surface microhardness (SMH) was measured 3 times; at baseline, before and after pH cycling using the microhardness tester (FM700 Future Tech Corp. Japan). The percentages of  ∆SMH were compared using One-way ANOVA and LSD test.

Results: The percentages of ∆SMH in each group were 21.85±8.3, 34.94±15.1, 54.00±16.9, and 51.45±18.1VHN respectively. All treatment groups had significantly higher rehardening effects than the control group and no difference between the chitosan added NaF mouthrinse and NaF mouthrinse.(p<0.05)

Conclusion: Chitosan mouthrinse showed a significant better effect on rehardening of enamel caries than negative control. However, the chitosan combined with NaF mouthrinse showed no difference effect from NaF mouthrinse.