Effects of Physicochemical Factors on Loss of Infectivity of Opisthorchis viverrini Cercariae

Naiyana  SENASRI; Smarn  TESANA; Chanisala  SEREEWONG; Jukkrid  CHAIYOS; Monticha  CHAIYASAENG; Sutee  WONGMANEEPRATEEP

doi:10.48048/wjst.2021.9481

Authors

Naiyana SENASRI Department of Fisheries, Faculty of Natural Resources, Rajamangala University of Technology Isan, Sakon Nakhon Campus, Sakon Nakhon 47160, Thailand
Smarn TESANA Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Chanisala SEREEWONG Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Jukkrid CHAIYOS Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Monticha CHAIYASAENG Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Sutee WONGMANEEPRATEEP Department of Fisheries, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand

DOI:

https://doi.org/10.48048/wjst.2021.9481

Keywords:

Cercariae, Combinations, Physicochemical factor, Opisthorchis viverrini

Abstract

This study aims to develop an alternative field-based approach to interrupt the life cycle of O. viverrini. The effects of temperature, salinity, acidity, ultraviolet A, B, C radiation, and combinations of these physicochemical factors on the loss of infectivity of Opisthorchis viverrini cercariae were analyzed to determine values required for 50 and 95 % lethal concentrations (LC₅₀ and LC₉₅) and period of loss infection success (LI₅₀ and LI₉₅). Newly shed cercariae of O. viverrini were used. LC₅₀ and LC₉₅ values for temperature, salinity, and acidity on cercariae were 33.19 and 37.70 ºC, 7.29 and 8.40 ppt, 4.62 and 4.80 M H₂CO₃, respectively. The values of LI₅₀ and LI₉₅ on cercariae by exposure to UVA, UVB, and UVC were 19.54 and 20.11 h, 5.03 and 5.12 h, 3.37 and 6.02 min, respectively. Combinations of these factors in the presence of UVC proved damaging to cercariae most rapidly. The shortest loss infection time of LI₅₀ and LI₉₅ were 1.09 and 2.83 min. Cercariae were deemed to have lost the ability to infect cyprinid fish when they had shed their tails, even though they were still capable of some movement. In nature, temperature, salinity, acidity, ultraviolet radiation, and combinations of these factors, affect the ability of O. viverrini cercariae to infect cyprinid fish.

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