Germination and Regeneration of Cymbidium findlaysonianum Lindl. on a Medium Supplemented with Some Organic Sources

Supavadee TAWARO; Potjamarn SURANINPONG; Sontichai CHANPRAME

Authors

Supavadee TAWARO Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla 90110
Potjamarn SURANINPONG Division of Crop Production Technology, School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161
Sontichai CHANPRAME Department of Agronomy, Faculty of Agriculture, Kasetsart University, Khamphaeng Saen Campus, Nakhon Pathom 73140

Keywords:

Cymbidium findlaysonianum, germination, organic additives, protocorm

Abstract

Seeds of Cymbidium findlaysonianum Lindl. were germinated and regenerated on media supplemented with various additives. After germination for 3 months, solidified Vacin and Went (VW) medium supplemented with, 15 % coconut water, 5 % banana homogenate, 5 % potato homogenate, 0.2 % activated charcoal and 20 g/l sucrose promoted higher seedling germination than the control medium. The number of protocorm cultured in a modified liquid VW medium with Murashige and Skoog (MS) vitamin (VWM) increased 4 times for every month of culture, a significant difference from the VW medium, MS medium and half strength MS medium (½ MS). Moreover, the activated charcoal added to the medium stimulated seedlings growth significantly more than when coconut water, banana homogenate and/or potato homogenate were added to the medium. Healthy plants transferred to a plastic tray containing coconut peat successfully acclimatized (70 %) in the greenhouse. More than 100,000 plantlets may be obtained from a capsule after being cultured for a year. Thus, organic additives and medium components had an effect on the growth and development of asymbiotic seeds in C. findlaysonianum.

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