Integron Expression in Multidrug-Resistant Escherichia coli Isolated from House Flies within the Hospital

Atchariya YOSBOONRUANG, Anong KIDDEE, Chatsuda BOONDUANG, Phannarai PIBALPAKDEE

Abstract


Escherichia coli is a serious cause of a variety of hospital-acquired infections and commonly contributes to the environment by house flies. Integrons, particularly class 1 integrons, are the genetic elements that play an important role in the horizontal transfer of antimicrobial resistance mechanism. This mechanism is commonly found in Enterobacteriaceae, especially E. coli. In this study, we aim to investigate the occurrence and antimicrobial resistance patterns of E. coli isolated from the house flies in Phayao hospital and to determine the gene expression of class 1 integrons in those isolates of E. coli. Totally, 70 isolates of E. coli were isolated from 60 house flies collected from the hospital. Fifty-seven of the isolates (81.43 %) were multidrug resistance (MDR) and highly resistant to b-lactams, tetracyclines, and sulfonamides. Of 57 isolates of MDR-E. coli, 20 isolates (35 %) were found to carry class 1 integron genes. Fifteen patterns of antimicrobial resistance occurred in the isolates of integron-positive E. coli. Most integron-positive E. coli isolates were resistant to 7 antimicrobials. Two isolates of these bacteria (10 %) were able to resist 13 out of 14 tested antimicrobials. Using PCR and sequencing analysis, an investigation showed that dfrA17-aadA5, dfrA12-aadA2 gene cassette was the most prevalent cassette (n = 10; 50 %) among the integron-positive E. coli isolates. Our results indicated that the presences of multidrug resistance and class 1 integrons were common in E. coli isolated from the houseflies in hospital. Therefore, screening for integron-positive E. coli from the hospital environment might be necessary for prevention of nosocomial infections.

Keywords


Antimicrobial resistance, class 1 integron, Escherichia coli, house flies, nosocomial spread

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References


Centers for Disease Control and Prevention. National strategy for combating antibiotic-resistant bacteria. Antibiotic Resistance Treats in the United States. 2013.

SH Hsu, TH Chiu, JC Pang, CH Hsuan-Yuan, GN Chang and HY Tsen. Characterisation of antimicrobial resistance patterns and class 1 integrons among Esherichia coli and Salmonella enterica serotype Cholesteraesuis strains isolated from humans and swine in Taiwan. Int. J. Antimicrob. Agents 2006; 27, 383-91.

AP Magiorakos, A Srinivasan, RB Carey, Y Carmeli, ME Falagas, CG Giske, S Harbarth, JF Hindler, G Kahlmeter, B Olsson-Liljequist, DL Paterson, LB Rice, J Stelling, MJ Struelens, A Vatopoulos, JT Weber and DL Monnet. Mutidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin. Microbiol. Infect. 2012; 18, 268-81.

MN Swartz. Human diseases caused by foodborne pathogens of animal origin. Clin. Infect. Dis. 2002; 34, S111-S122.

S Cocchi, E Grasselli, M Gutacker, C Benagli, M Convert and JC Piffaretti. Distribution and characterization of integrons in Escherichia coli strains of animal and human origin. FEMS Immunol. Med. Microbiol. 2007; 50, 126-32.

AC Fluit and FJ Schmitz. Resistance integrons and super-integrons. Clini. Microbiol. Infect. 2004; 10, 272-88.

P Suntaravitun. Flies: The important role in medicine. Songklanagarind Med. J. 2012; 30, 167-78.

JF Butler, AG Maruniak, F Meek and JE Maruniak. Wild Florida house flies (Musca domestica) as carriers of pathogenic bacteria. Fla. Entomol. 2010; 93, 218-23.

FC Onwugamba, JR Fitzgerald, K Rochon, L Guardabassi, A Alabi, S Kühneg, MP Grobusch and F Schaumburg. The role of ‘filth flies’ in the spread of antimicrobial resistance. Travel. Med. Infect. Dis. 2018; 22, 8-17.

R Ranjbar, M Izadi, TT Hafshejani and F Khamesipour. Molecular detection and antimicrobial resistance of Klebsiella pneumoniae from house flies (Musca domestica) in kitchens, farms, hospitals and slaughterhouses. J. Infect. Public Health 2016; 9, 499-505.

TDS Alves, GHB Lara, RP Maluta, MG Ribeiro and DDS Leite. Carrier flies of multidrug-resistant Escherichia coli as potential dissemination agent in dairy farm environment. Sci. Total Environ. 2018; 633, 1345-51.

J Rybaríková, M Dolejská, D Materna, I Literák and A Cízek. Phenotypic and genotypic characteristics of antimicrobial resistant Escherichia coli isolated from symbovine flies, cattle and sympatric insectivorous house martins from a farm in the Czech Republic (2006-2007). Res. Vet. Sci. 2010; 89, 179-89.

F Schaumburg, FC Onwugamba, R Akulenko, G Peters, A Mellmann, R Köck and K Becker. A geospatial analysis of flies and the spread of antimicrobial resistant bacteria. Int. J. Med. Microbiol. 2016; 306, 566-71.

ES Lestari, JA Severin and H Verbrugh. Antibacterial resistance among pathogenic bacteria in Southeast Asia. Southeast Asian J. Trop. Med. 2012; 43, 385-422.

S Pitikultang, CH Munsawaengsub and CH Chanyasanha. Factors associated with pharyngeal carriage of Streptococcus pneumoniae and Antimicrobial resistance in healthy children attending day-care center of a health promotional hospital. J. Public Health 2010; 40, 123-35.

P Pattarach, S Pitikultang, CH Chanyasanha and D Sujirarat. Staphylococcus aureus and methicillin-resistant Staphylococcus aureus among outpatients with skin and soft tissue infections at Prachuaphhirikhan general, Hua Hin and Pranburi hospital. J. Public Health 2011; 40, 173-82.

A Yosboonruang, C Jitsatthra and L Tharawet. Incidence of resistant bacteria isolated from medical devices in intensive care unit of the hospital. J. Public Health 2016; 47, 222-4.

Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-fourth Informational Supplement. Document M100-S24. Wayne, PA, 2014.

N Shibata, Y Doi, K Yamane, T Yagi, H Kurokawa, K Shibayama, H Kato, K Kai and Y Arakawa. PCR typing of genetic determinants for metallo-beta-lactamases and integrases carried by gram-negative bacteria isolated in Japan, with focus on the class 3 integron. J. Clin. Microbiol. 2003; 41, 5407-13.

C Lévesque, L Piché, C Larose and PH Roy. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob. Agents Chemother. 1995; 39, 185-91.

S Phongpaichit, K Wuttananupan and W Samasanti. Class 1 integrons and multidrug resistance among Escherichia coli isolates from human stools. Southeast Asian J. Trop. Med. 2008; 39, 279-87.

HC Su, GG Ying, R Tao, RQ Zhang, JL Zhao and YS Liu. Class 1 integrons, sul resistance genes and antibiotic resistance in Escherichia coli isolated from Dongjiang River, South. China Environ. Pollut. 2012; 169, 42-9.

E Tajbakhash, F Khamesipour, R Ranjbar and IF Ugwu. Prevalence of class 1 and 2 integrons in multi-drug resistant Escherichia coli isolated from aquaculture water in Chaharmahal Va Bakhtiari province, Iran. Ann. Clin. Microbiol. Antimicrob. 2015; 14, 1-5.

R Koczura, J Mokracka, L Jabłońska, E Gozdecka, M Kubek and A Kaznowski. Antimicrobial resistance of integron-harboring Escherichia coli isolates from clinical samples, wastewater treatment plant and river water. Sci. Total Environ. 2012; 414, 680-5.

LL Chang, TM Chang and CY Chang. Variable gene cassette patterns of class 1 integron-associated drug-resistant Escherichia coli in Taiwan. Kaohsiung J. Med. Sci. 2007; 23, 273-80.

SR Norrby. Integrons: Adding another threat to the use of antibiotic therapy. Clin. Infect. Dis. 2005; 41, 10-1.


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