Development of Anti-Acne Film from Bio-Cellulose Incorporating Punica granatum Peel Extract


  • Preeyawass PHIMNUAN Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000
  • Swanya YAKAEW Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000
  • Atchariya YOSBOONRUANG Department of Microbiology, Faculty of Medical Sciences, University of Phayao, Phayao 56000
  • Witoo LUANGBUDNAK Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000
  • Francois GRANDMOTTET Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000
  • Jarupa VIYOCH Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000



Pomegranate peel extract, Bio-cellulose, Acetobactor xylinum, Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes


The aim of this study was to formulate bio-cellulose film incorporating Punica granatum (pomegranate) peel extract for application as anti-acne product. The dried pomegranate peels were extracted by water (PPEwater), 50 % (PPE50E), 70 % (PPE70E) or 95 % (PPE95E) (v/v) ethanol. The extracts were total phenolic content (TPC) and free radical scavenging activity. PPE50E had highest TPC (55.95±1.44 gGAE/100g of dried peel extract), highest free radical scavenging activity with IC50 of 18.77±1.73 µg/ml and exerted the activity of bactericidal against Staphylococcus aureus, Staphylococcus epidermidis and Propionibacterium acne (P. acne). The PPE50E was selected for formulating anti-acne bio-cellulose (BC) film. The BC film prepared from bacteria Acetobacter xylinum. It showed a strong film with the tensile strength in range of 5 - 15 MPa and the elongation at break about 5 %. The simple soaking method was used to combine BC with 5 or 10 mg/ml of PPE50E. By the SEM, the assemble nano-fibrils and the layer structure of the BC combining with the PPE50E (BCP) was found. The BCP had higher tensile strength and lower elongation at break than the BC. The BCP at wet state showed a soft and flexible film. The anti-bacterial efficacy of the BCP against tested bacteria was observed, according to the disc diffusion assay. The P. acne inhibition zones of the BCP at the concentration of 5 and 10 mg/ml were 27.7 and 35.5 mm which closed to the clindamycin (45.5 mm) as positive control. From the remarkable results, the BCP is interestingly natural device for acne treatment.


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JJ Leyden. Therapy for acne vulgaris. N. Engl. J. Med. 1997; 16, 1156-62.

AP Desbois and KC Lawlor. Antibacterial activity of long-chain polyunsaturated fatty acids against Propionibacterium acnes and Staphylococcus aureus. Mar. Drugs 2013; 11, 4544-57.

G Sibi. Inhibition of lipase and inflammatory mediators by Chlorella lipid extracts for anti-acne treatment. J. Adv. Pharm. Tech. Res. 2015; 6, 7-12.

V Patil, A Bandivadekart and D Debjani. Inhibition of Propionibacterium acnes lipase by extracts of Indian medicinal plants. Int. J. Cosmet. Sci. 2012; 34, 234-9.

A Andriessen and CW Lynde. Antibiotic resistance: Shifting the paradigm in topical acne treatment. J. Drugs. Dermatol. 2014; 13, 1358-64.

A Zarfeshany, S Asgary and SH Javanmard. Potent health effects of pomegranate. Adv. Biomed. Res. 2014; 3, 100.

P Panichayupakaranant, S Tewtrakul and S Yuenyongsawad. Antibacterial, anti-inflammatory and anti-allergic activities of standardised pomegranate rind extract. Food Chem. 2010; 2, 400-3.

A Scalbert. Antimicrobial properties of tannins. Phytochemistry 1991; 12, 3875-83.

Z Li, PH Summanen, J Downes, K Corbett, T Komoriya, SM Henning, J Kim and SM Finegold. Antimicrobial activity of pomegranate and green tea extract on Propionibacterium acnes, Propionibacterium granulosum, Staphylococcus aureus, Staphylococcus epidermidis. J. Drugs Dermatol. 2015; 14, 574-8.

CJ Lee, LG Chen, WL Liang and CC Wang. Multiple activities of Punica granatum Linne against acne vulgaris. Int. J. Mol. Sci. 2017; 18, 141.

S Malviya, Arvind, A Jha and N Hettiarachchy. Anti-oxidant and antibacterial potential of pomegranate peel extracts. J. Food Sci. Tech. 2014; 51, 4132-7.

PS Negi and GK Jayaprakasha. Anti-oxidant and antibacterial activities of Punica granatum peel extracts. J. Food Sci. 2003; 684, 1473-7.

Y Li, C Guo, J Yang, J Wei, J Xu and S Cheng. Evaluation of anti-oxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem. 2006; 96, 254-60.

T Nuamsetti, P Dechayuenyong and S Tantipaibulvut. Antibacterial activity of pomegranate fruit peels and arils. ScienceAsia 2012; 38, 319-22.

H Zhai and HI Maibach. Occlusion vs. skin barrier function. Skin Res. Tech. 2002; 8, 1-6.

FG Torres, S Commeaux and OP Troncoso. Biocompatibility of bacterial cellulose based biomaterials. J. Funct. Biomater. 2012; 3, 864-78.

N Lin and A Dufresne. Nanocellulose in biomedicine: Current status and future prospect. Eur. Polym. J. 2014; 59, 302-25.

Z Wang, Z Pan, H Ma and GG Atungulu. Extract of phenolics from pomegranate peels. Open Food. Sci. J. 2011; 5, 17-25.

J Viyoch, K Mahingsa and K Ingkaninan. Effects of Thai Musa species on prevention of UVB-induced skin damage in mice. Food Chem Toxicol. 2012; 50, 4292-301.

K Itsarasook, K Ingkaninan and J Viyoch. Artocarpin-enriched extract reverses collagen metabolism in UV-exposed fibroblasts. Biologia 2014; 69, 943-51.

J Viyoch, N Pisutthanan, A Faikreua, K Nupangta, K Wangtorpol and J Ngokkuen. Evaluation of in vitro antimicrobial activity of Thai basil oils and their micro‐emulsion formulas against Propionibacterium acnes. Int. J. Cos. Sci. 2006; 28, 125-33.

SG Jenkins and AN Schuetz. Current concept in laboratory testing to guide antimicrobial therapy. Mayo. Clin. Proc. 2012; 87, 290-308.

COLIPA. The European Cosmetics Association, Guidelines on Stability Testing of Cosmetic Products. 2004.

RP Vieira, AR Fernandes, TM Kaneko, VO Consiglieri, CASdO Pinto, CSC Pereira, AR Baby and MVR Velasco. Physical and physicochemical stability evaluation of cosmetic formulations containing soybean extract fermented by Bifidobacterium animalis. Braz. J. Pharm. Sci. 2009; 45, 515-25.

D Lerche and T Sobisch. Direct and accelerated characterization of formulation stability. J. Disp. Sci. Tech. 2011; 32, 1799-811.

MC Cláudio, LAdOSilva, JG Amaral and MF Leite. Development, characterization and stability study of nanometric systems containing hydrogen peroxide for dental bleaching. Int. J. Pharm. Sci. Res. 2017; 8, 4551-64.

A Vidal, A Fallarero, BR Peña, ME Medina, B Gra, F Rivera, Y Gutierrez and PM Vuorela. Studies on the toxicity of Punica granatum L. (Punicaceae) whole fruit extracts. J. Ethnopharmacol. 2003; 89, 295-300.

J Viyoch, K Mahingsa and K Ingkaninan. Effects of Thai Musa species on prevention of UVB-induced skin damage in mice. Food. Chem. Toxicol. 2012; 50, 4292-301.

QD Do, AE Angkawijaya, PL Tran-Nguyen, LH Huynh, FE Soetaredjo, S Ismadji and YH Ju. Effect of extraction solvent on total phenol content, total flavonoid content, and anti-oxidant activity of Limnophila aromatica. J. Food. Drug. Anal. 2014; 22, 296-302.

M Alothman, R Bhat and AA Karim. Anti-oxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food. Chem. 2009; 115, 785-8.

D Babu, P Gurumurthy, SK Borra and KM Cherian. Anti-oxidant and free radical scavenging activity of triphala determined by using different in vitro models. J. Med. Plants. Res. 2013; 7, 2898-905.

K Mitra and N Uddin. Total phenolics, flavonoids, proanthrocyanidins, ascorbic acid contents and in vitro anti-oxidant activities of newly developed isolated soya protein. Dis. J. Agri. Food. Sci. 2014; 2, 160-8.

OH Mills, MC Criscito, TE Schlesinger, R Verdicchio and E Szoke. Addressing free radical oxidation in acne vulgaris. J. Clin. Aesthet. Dermatol. 2016; 9, 25-30.

S Briganti and M Picardo. Anti-oxidant activity, lipid peroxidation and skin diseases: What’s new? J. Eur. Acad. Dermatol. Venereol. 2003; 17, 663-39.

PR Murray and JH Jorgensen. Quantitative susceptibility test methods in major United States medical centers. Antimicrob. Agents Chemother. 1981; 20, 66-70.

LR Peterson and CJ Shanholtzer. Tests for bactericidal effects of antimicrobial agents: Technical performance and clinical relevance. Clin. Microbiol. Rev. 1992; 5, 420-32.

B Gullon, ME Pintado, JA Pérez-Álvarez and M Viuda-Martos. Assessment of polyphenolic profile and antibacterial activity of pomegranate peel (Punica granatum) flour obtained from co-product of juice extraction. Food. Control. 2016; 59, 94-8.

C Pagliarulo, Vd Vito, G Picariello, R Colicchio, G Pastore, P Salvatore and MG Volpe. Inhibitory effect of pomegranate (Punica granatum L.) polyphenol extracts on the bacterial growth and survival of clinical isolates of pathogenic Staphylococcus aureus and Escherichia coli. Food. Chem. 2016; 190, 824-31.

S Abdollahzadeh, RY Mashouf, H Mortazavi, MH Moghaddam, N Roozbahani and M Vahedi. Antibacterial and antifungal activities of Punica granatum peel extracts against oral pathogens. J. Dent. 2011; 8, 1-6.

X Su, AB Howell and DH D'Souza. Antibacterial effects of plant-derived extracts on methicillin-resistant Staphylococcus aureus. Foodborne. Pathog. Dis. 2012; 9, 573-8.

NS Al-Zoreky. Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. Int. J. Food Microbiol. 2009; 134, 244-8.

S Yamanaka and K Watanabe. Applications of Bacterial Cellulose. In: RD Gilbert (ed.). Cellulosic Polymers: Blends and Composites. Hanser Publishers, New York, 1994, p. 207-15.

ST Schrecker and PA Gostomski. Determining the water holding capacity of microbial cellulose. Biotechnol. Lett. 2005; 27, 1435-8.

M Iguchi, S Yamanaka and A Budhiono. Bacterial cellulose: A masterpiece of nature's arts. J. Mater. Sci. 2000; 35, 261-70.

M Phisalaphong, T Suwanmajo and P Tammarate. Synthesis and characterization of bacterial cellulose/alginate blend membranes. J. Appl. Polym. Sci. 2008; 107, 3419-24.




How to Cite

PHIMNUAN, P., YAKAEW, S., YOSBOONRUANG, A., LUANGBUDNAK, W., GRANDMOTTET, F., & VIYOCH, J. (2018). Development of Anti-Acne Film from Bio-Cellulose Incorporating Punica granatum Peel Extract. Walailak Journal of Science and Technology (WJST), 16(10), 765–778.



Research Article