Antioxidant and Anti-inflammatory Protective Properties of Thai Shallot (Allium ascalonicum cv. Chiangmai) Juice on Human Vascular Endothelial Cell Lines (EA.hy926)


  • Sakaewan OUNJAIJEAN NCD Center of Excellence, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200
  • Sukanya CHACHIYO Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200
  • Kanokwan KULPRACHAKARN NCD Center of Excellence, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200
  • Kongsak BOONYAPRANAI Faculty of Medical Science, Nakhonratchasima College, Nakhonratchasima 30000
  • Somdet SRICHAIRATANAKOOL Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200
  • Kittipan RERKASEM Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200



Shallots, Allium ascalonicum, antioxidant activity, anti-inflammatory activity, endothelial cells


Oxidative stress and inflammation are 2 major contributors to numerous life-threatening disorders, including vascular pathologies. Shallots (Allium ascalonicum) are a type of red onion which grows in Southeast Asia. Bulbs of this plant are used both as a food ingredient and in traditional medicine. This study attempted to investigate the possible ways that juice extracted from Thai shallot (A.ascalonicum cv. Chiangmai) bulbs could be used in the prevention of cardiovascular complications. The antioxidative and anti-inflammatory effects of shallot juice extract (SHE) on human vascular endothelial cells (EA.hy926) were investigated. Cell viability was evaluated by MTT assay, membrane lipid peroxidation by thiobarbituric acid reactive substances assay, intracellular reactive oxygen species (ROS) production by the fluorescent probe 6-carboxy-2'-7'-dichlorofluoresceine, and interleukin-6 (IL-6) released by ELISA. The shallot juice showed extremely low cytotoxicity against EA.hy926 cells, with IC50 of 41.9 and 27.3 mg/ml for 24 h- and 48 h-incubation, respectively. SHE reduced the iron-induced malondialdehyde production in a dose-dependent manner. The extract also demonstrated antioxidant activity as shown by a significant reduction of H2O2-induced ROS production at a low concentration (< 200 mg/ml). Furthermore, SHE significantly attenuated the level of IL-6 released during lipopolysaccharide stimulation (p < 0.05). It is of interest that the juice extracted from Thai shallot bulbs demonstrated both cellular antioxidant and anti-inflammatory properties in endothelial cell models, combined with a reduction in toxicity. Shallot extract could be considered as a nutraceutical for the prevention or management of vascular diseases as it is related to oxidative stress and inflammation.


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P Adegbola, I Aderibigbe, W Hammed and T Omotayo. Antioxidant and anti-inflammatory medicinal plants have potential role in the treatment of cardiovascular disease: a review. Am. J. Cardiovasc. Dis. 2017; 7, 19-32.

P Pignatelli, D Menichelli, D Pastori and F Violi. Oxidative stress and cardiovascular disease: New insights. Kardiol. Pol. 2018; 76, 713-22.

AO Ademosun, G Oboh, F Bello and PO Ayeni. Antioxidative properties and effect of quercetin and its glycosylated form (Rutin) on acetylcholinesterase and butyrylcholinesterase activities. J. Evid Based Compl. Alternat. Med. 2016; 21, NP11-7.

World Health Organization (WHO). WHO Guidelines on Safety Monitoring of Herbal Medicines in Pharmacovigilance Systems. World Health Organization, Geneva, Switzerland, 2004.

HR Mohammadi-Motlagh, A Mostafaie and K Mansouri. Anticancer and anti-inflammatory activities of shallot (Allium ascalonicum) extract. Arch. Med. Sci. AMS 2011; 7, 38-44.

E Fattorusso, M Iorizzi, V Lanzotti and O Taglialatela-Scafati. Chemical composition of shallot (Allium ascalonicum Hort.). J. Agric. Food Chem. 2002; 50, 5686-90.

P Mikaili, S Maadirad, M Moloudizargari, S Aghajanshakeri and S Sarahroodi. Therapeutic uses and pharmacological properties of garlic, shallot, and their biologically active compounds. Iran J. Basic Med. Sci. 2013; 16, 1031-48.

VL Singleton, R Orthofer, RM Lamuela-Raventos. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu Reagent. Meth. Enzymol. 1999; 299, 152-78.

J Zhishen, T Mengcheng and W Jianming. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999; 64, 555-9.

R Re, N Pellegrini, A Proteggente, A Pannala, M Yang and C Rice-Evans. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 1999; 26, 1231-7.

K Kulprachakarn, K Pangjit, C Phisalaphong, S Fucharoen, RC Hider and S Srichairatanakool. Combination treatments of 1-(N-Acetyl-6-Aminohexyl)-3-Hydroxy-2-Methylpyridin-4-One (CM1) with deferiprone and desferrioxamine reduced labile iron pool and protected oxidative stress in iron-loaded cultured hepatocytes. Vitam. Miner. 2013; 2013, 115.

J Amer, A Goldfarb and E Fibach. Flow cytometric measurement of reactive oxygen species production by normal and thalassaemic red blood cells. Eur. J. Haematol. 2003; 70, 84-90.

B Halliwell and S Chiroco. Lipid peroxidation: Its mechanism, measurement, and significance. Am. J. Clin. Nutr. 1993; 57, 715S-725S.

AO Ademosun, G Oboh, S Passamonti, F Tramer, L Ziberna, AA Boligon, et al. Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial EA.Hy 926 cells. Food Sci. Human Wellness 2015; 4, 80-5.

HN Siti, Y Kamisah and J Kamsiah. The role of oxidative stress, antioxidants and vascular inflammation in cardiovascular disease: A review. Vasc. Pharmacol. 2015; 71, 40-56.

S Chachiyo, K Boonyapranai, S Srichairatanakool, R Rerkasem and S Ounjaijean. The effect of preparation method on total phenolic content and antioxidant activity of shallot (Alluim ascalonicum) extract. In: Proceedings of the 10th Thailand Congress of Nutrition. Bangkok, Thailand.

N Leelarungrayub, V Rattanapanone, N Chanarat and JM Gebicki. Quantitative evaluation of the antioxidant properties of garlic and shallot preparations. Nutrition 2006; 22, 266-74.

ES Abdel-Hameed, A Salih, SA Bazaid, MM Shohayeb, MM El-Sayed and EA El-Wakil. Phytochemical studies and evaluation of antioxidant, anticancer and antimicrobial properties of Conocarpus erectus L. growing in Taif, Saudi Arabia. Eur. J. Med. Plants 2012; 2, 93-112.

B Kalyanaraman, V Darley-Usmar, KJ Davies, PA Dennery, HJ Forman, MB Grisham, GE Mann, K Moore, LJ Roberts and H Ischiropoulos. Measuring reactive oxygen and nitrogen species with fluorescent probes: Challenges and limitations. Free Radic. Biol. Med. 2012; 52, 1-6.

Y Shen, G Chen, A Xiao, Y Xie, L Liu and Y Cao. In vitro effect of flaxseed oil and alpha-linolenic acid against the toxicity of lipopolysaccharide (LPS) to human umbilical vein endothelial cells. Inflammopharmacology 2018; 26, 645-54.

N Panth, KR Paudel and K Parajuli. Reactive oxygen species: A key hallmark of cardiovascular disease. Adv. Med. 2016; 2016, 9152732.

CM Sena, AM Pereira and R Seica. Endothelial dysfunction: A major mediator of diabetic vascular disease. Biochim. Biophys. Acta 2013; 1832, 2216-31.




How to Cite

OUNJAIJEAN, S., CHACHIYO, S., KULPRACHAKARN, K., BOONYAPRANAI, K., SRICHAIRATANAKOOL, S., & RERKASEM, K. (2019). Antioxidant and Anti-inflammatory Protective Properties of Thai Shallot (Allium ascalonicum cv. Chiangmai) Juice on Human Vascular Endothelial Cell Lines (EA.hy926). Walailak Journal of Science and Technology (WJST), 16(3), 175–184.