Free Radical Scavenging Activity and Reducing Capacity of Five Southern Thai Indigenous Vegetable Extracts

Worawan PANPIPAT, Weerayuth SUTTIRAK, Manat CHAIJAN

Abstract


The phenolic compounds of five southern Thai indigenous vegetables including Mon-pu (Glochidion wallichianum Muell Arg), Cha-plu (Piper sarmentosum Roxb.), white popinac (Leucaena leucocephala de Wit.), djenkol tree (Archidendrom jiringa I. C. Nielsen.) and stink bean (Parkia speciosa Hassk.) were extracted using different solvents (50 % acetone, 80 % methanol and distilled water) at a ratio of sample to extracting medium of 1:25 (w/v). The extracts were analyzed for total phenolic content using the Folin-Ciocalteu procedure, free radical scavenging capacity by using 2’,2’-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) methods, and reducing capacity by using a ferric reducing antioxidant power (FRAP) assay. The acetone extracts of all plants possessed the highest total phenolic content followed by the methanolic and distilled water extracts, respectively. The highest total phenolic content was found in the acetone extracts of Mon-pu and white popinac (p < 0.05). The acetone extracts of all plants showed higher free radical scavenging capacity and reducing capacity than those of their methanolic and water extracts counterparts, respectively (p < 0.05). Among all plants tested, the extracts of Mon-pu extracted with all extracting media exhibited the highest free radical scavenging and reducing abilities (p < 0.05). The present study suggested that the acetone extract of Mon-pu was a potential source of natural antioxidants.


Keywords


Free radical scavenging activity, reducing capacity, local vegetables, extracts, phenolic compounds

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References


B Halliwell, JMC Gutteridge and CE Cross. Free radicals, antioxidants and human disease: where are we now? J. Lab. Clin. Med. 1992; 119, 598-620.

K Yaki. Lipid peroxides and human disease. Chem. Phys. Lipids. 1987; 45, 337-41.

T Finkel and NJ Holbrook. Oxidants, oxidative stress and the biology of aging. Nature. 2000; 408, 239-47.

L Su, JJ Yin, D Charles, K Zhou, J Moore and L Yu. Total phenolic contents, chelating capacities, and radicals-scavenging properties of black peppercorn, nutmeg, rosehip, cinnamon and oregano leaf. Food Chem. 2007; 100, 990-7.

MJ Stampfer, CH Henneekens, JE Manson, GA Colditz, B Rosner and WC Willet. Vitamin E consumption and the risk of coronary heart disease in women. New. Eng. J. Med. 1993; 328, 1444-9.

E Middleton, CH Kandaswamy and TC Theoharide. The effects of plant flavonoids on mammalian cells; Implication for inflammation, heart disease, and cancer. Pharm Rev. 2000; 52, 673-751.

KT Chung, TY Wong, YW Huang and Y Lin. Tannins and human health: a review. CRC Crit. Rev. Food Sci. Nutr. 1998; 38, 421-64.

A Cassidy, B Hanley and RM Lamuela-Raventos. Isoflavones, lignans and stilbenes-origins, metabolism and potential importance to human health. J. Sci. Food Agri. 2000; 80, 1044-62.

M Oktay, I Guloin and OI Kufrevioglu. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT Food Sci. Tech. 2003; 36, 263-71.

AA Van der Sluis, M Dekker, G Skrede and WMF Jongen. Activity and concentration of polyphenolic antioxidants in apple juice. I. Effect of existing production methods. J. Agri. Food Chem. 2002; 50, 7211-9.

H Shi, N Noguchi and N Etsuo. Introducing Natural Antioxidants. In: J Pokorny N Yanishlieva and M Gordon (eds.). Antioxidants in food: practical applications. CRC press, New York, 2001.

CA Rice-Evans, NJ Miller and G Paganga. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Bio. Med. 1996; 20, 933-56.

KE Heim, AR Tagliaferro and DJ Bobilya. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J. Nutr. Biochem. 2002; 13, 572-84.

L Yu, S Haley, J Perret, M Harris, J Wilson and M Qian. Free radical scavenging properties of wheat extracts. J. Agri. Food Chem. 2002; 50, 1619-24.

GA Agbor, JA Vinson, JE Oben and JY Ngogang. Comparative analysis of the in vitro antioxidant activity of white and black pepper. Nutri Res. 2006; 26, 659-63.

YZ Cai, Q Luo, M Sun and H Corke. Antioxidant activity and phenolic compounds of 112 Chinese medicinal plants associated with anticancer. Life Sci. 2004; 74, 2157-84.

YZ Cai, M Sun, J Xing, Q Luo and H Corke. Structure-radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants. Life Sci. 2006; 78, 2872-88.

I Gulcin, S Beydemir, HA Alici, M Elmastas and ME Buyukokurolu. In vitro antioxidant properties of morphine. Phar. Res. 2004; 49, 59-66.

T Sawa, M Nkao, T Akaike, K Ono and H Maeda. Alkyl-peroxyl radical scavenging activity of various flavonoids and other phenolic compounds: implications for the antitumor promoter effect of vegetables. J. Agri. Food Chem. 1999; 47, 397-402.

JM Lee, H Chung, PS Chang and JH Lee. Development of a method predicting the oxidative stability of edible oils using 2,2’-diphenyl-1-picrylhydrazyl (DPPH). Food Chem. 2007; 103, 662-9.

S Surveswaran, YZ Cai, H Corke and M Sun. Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chem. 2007; 102, 938-53.

P Maisuthisakul, M Suttajit and R Pongsawatmanit. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem. 2007; 100, 1409-18.

CW Chen and CT Ho. Antioxidant properties of polyphenols extracted from green and black tea. J. Food Lipid. 1995; 2, 35-46.

A Van Gadow, E Joubert and CF Hansmann. Comparison of the antioxidant activity of aspalathin with that of other plant phenols of rooibos tea (Asalathus linearis), -tocopherol, BHT and BHA. J. Agri. Food Chem. 1997; 45, 632-8.

K Schlesier, M Harwat, V Bohm and R Bitsch. Assessment of antioxidant activity by using different in vitro methods. Free Radic Res. 2002; 36, 177-87.

GC Yen, PD Duh and CL Tsai. Relationship between antioxidant activity and maturity of peanut hulls. J. Agri. Food Chem. 1993; 41, 67-70.

P Shiddhuraju, PS Mohan and K Becker. Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. Food Chem. 2002; 79, 61-7.

P Shiddhuraju and K Becker. Antioxidant properties of various solvent extracts of total phenolic constitutes from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J. Agri. Food Chem. 2003; 51, 2144-55.

DW Reische, DA Lillard and RR Eitenmiller. Antioxidants. In: CC Akoh and DB Min (eds.). Food lipids: chemistry, nutrition and biotechnology. CRC Press, New York, 2007, p. 409-30.


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