Potential Application of Ascorbic Acid, Citric Acid and Oxalic Acid for Browning Inhibition in Fresh-Cut Fruits and Vegetables



The market for fresh-cut fruits and vegetables has grown rapidly in recent decades as a result of their freshness, convenience, and human health benefits. However, fresh fruits and vegetables deteriorate very rapidly after processing, especially cut-surface browning resulting from wound-induced physiological and biochemical changes. The application of antibrowning agents is one of the most effective methods for controlling the enzymatic browning reaction in fresh-cut fruits and vegetables. This article reviews the use of nature identical antibrowning agents, which are generally recognized as safe (GRAS) including ascorbic acid, citric acid and oxalic acid for preventing browning in fresh-cut fruits and vegetables. Factors affecting inhibitory efficiency of the antibrowning agents and synergistic effects of the mixtures in various fresh-cut fruits and vegetables are presented.


Ascorbic acid, citric acid, oxalic acid, enzymatic browning, fresh-cut fruits and vegetables, polyphenol oxidase

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KB Michels, E Giovannucci, KJ Joshipura, BA Rosner, MJ Stampfer, CS Fuchs, GA Colditz, FE Speizer and WC Willett. Prospective study of fruit and vegetable consumption and incidence of colon and rectal cancer. J. Natl. Cancer Inst. 2000; 92, 1740-52.

NJ Temple and KK Gladwin. Fruit, vegetables, and the prevention of cancer: research challenges. Nutr. Res. Rev. 2003; 19, 467-70.

A Del Caro, A Piga, V Vacca and M Agabbio. Changes of flavonoids, vitamin C, and antioxidant capacity in minimally processed citrus segments and juices during storage. Food Chem. 2004; 84, 99-105.

C Kaur and HC Kapoor. Antioxidants in fruits and vegetables: The millennium’s health. Int. J. Food Sci. Technol. 2001; 36, 703-25.

LP Leong and G Shui. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem. 2002; 76, 69-75.

EH Garrett. Fresh-cut Produce: Tracks and Trends. In: Lamikanra O (ed.). Fresh-cut Fruits and Vegetables: Science, Technology, and Market. CRC Press, Boca Raton, 2002, p. 1-10.

HL Jeong, WJ Jin, DM Kwang and JP Kee. Effects of anti-browning agents on polyphenoloxidase activity and total phenolics as related to browning of fresh-cut ‘Fuji’ apple. ASEAN Food J. 2008; 15, 79-87.

JR Gorny, B Hess-Pierce, RA Cifuentes and AA Kader. Quality changes in fresh-cut pear slices as affected by controlled atmospheres and chemical preservatives. Postharvest Biol. Technol. 2002; 24, 271-8.

SM Son, KD Moon and CY Lee. Inhibitory effects of various antibrowning agents on apple slices. Food Chem. 2001; 73, 23-30.

JY Lee, HJ Park, CY Lee and WY Choi. Extending shelf-life of minimally processed apples with edible coatings and antibrowning agents. Lebensm. Wiss. Technol. 2003; 36, 323-9.

GA González-Aguilar, S Ruiz-Cruz, R Cruz-Valenzuela, A Rodríguez-Félix and CY Wang. Physiological and quality changes of fresh-cut pineapple treated with antibrowning agents. Lebensm. Wiss. Technol. 2004; 37, 369-76.

AA Kader. Quality Parameters of Fresh-cut Fruit and Vegetable Products. In: Lamikanra O (ed.). Fresh-cut Fruits and Vegetables: Science, Technology, and Market. CRC Press, Boca Raton, 2002, p. 11-20.

D Rico, AB Martín-Diana, JM Barat and C Barry-Ryan. Extending and measuring the quality of fresh-cut fruit and vegetable: a review. Trends Food Sci. Technol. 2007; 18, 373-86.

E Garcia and DM Barrett. Preservative Treatments for Fresh-cut Fruits and Vegetables. In: Lamikanra O (ed.). Fresh-cut Fruits and Vegetables: Science, Technology, and Market. CRC Press, Boca Raton, 2002, p. 267-83.

KL Parkin. Enzymes. In: Damodaran S, Parkin KL and Fennema OR (eds.). Fennema’s Food Chemistry. 4th ed. CRC Press, New York, 2008, p. 407-12.

D Kavrayan and T Aydemir. Partial purification and characterization of polyphenol oxidase from peppermint (Mentha piperita). Food Chem. 2001; 74, 147-54.

S Jolivet, N Arpin, HJ Wichers and G Pellon. Agaricus bisporus browning: a review. Mycol. Res. 1998; 102, 1459-83.

PMA Toivonen and DA Brummell. Biochemical bases of appearance and texture changes in fresh-cut fruit and vegetables. Postharvest Biol. Technol. 2007; 48, 1-14.

H Li, A Guo and H Wang. Mechanisms of oxidative browning of wine. Food Chem. 2008; 108, 1-13.

A Altunkaya and V Gökmen. Effect of various anti-browning agents on phenolic compounds profile of fresh lettuce (L. sativa). Food Chem. 2009; 117, 122-6.

A Altunkaya and V Gökmen. Effect of various inhibitors on enzymatic browning, antioxidant activity and total phenol content of fresh lettuce (Lactuca sativa). Food Chem. 2008; 107, 1173-9.

V Kabasakalis, D Siopidou and E Moshatou. Ascorbic acid content of commercial fruit juices and its rate of loss upon storage. Food Chem. 2000; 70, 325-8.

R Pernice, G Borriello, R Ferracane, RC Borrelli, F Cennamo and A Ritieni. Bergamot: A source of natural antioxidants for functionalized fruit juices. Food Chem. 2009; 112, 545-50.

G del Campo, I Berregi, R Caracena and JI Santos. Quantitative analysis of malic and citric acids in fruit juices using proton nuclear magnetic resonance spectroscopy. Anal. Chim. Acta. 2006; 556, 462-8.

TW Naudé and V Naidoo. Oxalates-containing plants. In: Gupta RC (ed.). Veterinary Toxicology: Basic and Clinical Principles. Academic Press, Oxford, 2007, p. 880-91.

T Kayashima and T Katayama. Oxalic acid is available as a natural antioxidant in some systems. Biochim. Biophys. Acta. 2002; 1573, 1-3.

F Pizzocaro, D Torreggiani and G Gilardi. Inhibition of apple polyphenoloxidase (PPO) by ascorbic acid, citric acid and sodium chloride. J. Food Process. Preserv. 1993; 17, 21-30.

RH Veltman, RM Kho, ACR Van Schaik, MG Sanders and J Oosterhaven. Ascorbic acid and tissue browning in pears (Pyrus communis L. cvs Rocha and Conference) under controlled atmosphere conditions. Postharvest Biol. Technol. 2000; 19, 129-37.

H Özoğlu and A Bayındırlı. Inhibition of enzymic browning in cloudy apple juice with selected antibrowning agents. Food Control. 2002; 13, 213-21.

I Lichanporn and S Kanlavanarat. Effect of organic acid and modified atmosphere conditions on quality of shredded green papaya (Carica papaya L.). Acta Hort. 2006; 712, 729-34.

N Pongsakul, B Leelasart and N Rakarlyatham. Effect of L-cysteine, potassium metabisulfite, ascorbic acid and citric acid on inhibition of enzymatic browning in Longan. Chiang Mai J. Sci. 2006; 33, 137-41.

X Zheng and S Tian. Effect of oxalic acid on control of postharvest browning of litchi fruit. Food Chem. 2006; 96, 519-23.

M Apintanapong, K Cheachumluang, P Suansawan and N Thongprasert. Effect of antibrowning agents on banana slices and vacuum-fried slices. J. Food Agric. Environ. 2007; 5, 151-7.

P Rocculi, FG Galindo, F Mendoza, L Wadsö, S Romani, MD Rosa and I Sjöholm. Effects of the application of anti-browning substances on the metabolic activity and sugar composition of fresh-cut potatoes. Postharvest Biol. Technol. 2007; 43, 151-7.

FN Arroyo-López, J Bautista-Gallego, MC Durán-Quintana and A Garrido Fernández. Effects of ascorbic acid, sodium metabisulfite and sodium chloride on freshness retention and microbial growth during the storage of Manzanilla-Aloreña cracked table olives. Lebensm. Wiss. Technol. 2008; 41, 551-60.

SLS Bico, MFJ Raposo, RMSC Morais and AMMB Morais. Combined effects of chemical dip and/or carrageenan coating and/or controlled atmosphere on quality of fresh-cut banana. Food Control. 2009; 20, 508-14.

Z Li-Qin, Z Jie, Z Shu-Hua and G Lai-Hui. Inhibition of browning on the surface of peach slices by short-term exposure to nitric oxide and ascorbic acid. Food Chem. 2009; 114, 174-9.

Pacific Health Sciences, Available at: http://www.pacifichealth.com/ gras_list.htm#GRAS, accessed October 2008.

YM Jiang. Purification and some properties of polyphenol oxidase of longan fruit. Food Chem. 1999; 66, 75-9.

AMCN Rocha and AMMB Morais. Characterization of polyphenoloxidase (PPO) extracted from ‘Jonagored’ apple. Food Control. 2001; 12, 85-90.

B Dincer, A Colak, N Aydin, A Kadioglu and S Günera. Characterization of polyphenol oxidase from medlar fruits (Mespilus germanica L., Rosaceae). Food Chem. 2002; 77, 1-7.

KM Mdluli. Partial purification and characterization of polyphenol oxidase and peroxidase from marula fruit (Sclerocarya birrea subsp. Caffra). Food Chem. 2005; 92, 311-23.

U Gawlik-Dziki, U Szymanowska and B Baraniak. Characterization of polyphenol oxidase from broccoli (Brassica oleracea var. botrytis italica) florets. Food Chem. 2007; 105, 1047-53.

T Aydemir. Partial purification and characterization of polyphenol oxidase from artichoke (Cynara sclymus L.). Food Chem. 2004; 87, 59-67.

T Aydemir and G Akkanlı. Purification and characterization of polyphenoloxidase from celery root (Apium graveolens) and the investigation on enzyme activity of some inhibitors. Int. J. Food Sci. Technol. 2006; 41, 1090-8.

SM Son, KD Moon and CY Lee. Kinetic study of oxalic acid inhibition on enzymatic browning. J. Agric. Food Chem. 2000; 48, 2071-4.

Y Jiang, L Pen and J Li. Use of citric acid for shelf life and quality maintenance of fresh-cut Chinese water chestnut. J. Food Eng. 2004; 63, 325-8.


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