Monitoring of Biochemical Compounds and Fatty Acid in Marine Microalgae from the East Coast of Thailand


  • Kwanchayanawish MACHANA Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131, Thailand
  • Amonrat KANOKRUNG The Institute of Marine Sciences, Burapha University, Chonburi 20131, Thailand
  • Sirinart SRICHAN Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima 30000, Thailand
  • Boonyadist VONGSAK Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131, Thailand
  • Maliwan KUTAKO Faculty of Marine Technology, Burapha University, Chonburi 20131, Thailand
  • Ekarin SIAFHA Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131, Thailand



Marine microalgae, Biochemical compounds, East coast of Thailand, Fatty Acid, FT-IR


Determinations of fatty acid profiles of five microalgae; Amphora sp., Chaetoceros sp., Melosira sp., Bellerochae sp., and Lithodesmium sp., from the east coast of Thailand were evaluated by conventional Gas Chromatography-Flame Ionization Detector (GC-FID). The results exhibited that the fatty acids suitable for biodiesel production were the most frequent entities encountered in all microalgae profiles. The GC chromatogram of fatty acid profiles in microalgae showed that both Amphora sp. and Chaetoceros sp. comprised essential omega-3 fatty acids, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Additionally, this study assessed whether Fourier Transform infrared (FT-IR) microspectroscopy could be used to evaluate and monitor the biochemical compositions of microalgae, including lipid, carbohydrate, and protein profiles, by using colorimetric methods. Results showed that FT-IR spectra combined with biochemical values of lipid, carbohydrate, and protein contents were used as predictive models generated by partial least square (PLS) regression. Cross-validation of the lipid, protein, and carbohydrate models showed high degrees of statistical accuracy with RMSECV values of approximately 0.5 - 3.22 %, and a coefficient of regression between the actual and predicted values of lipids, carbohydrates, and proteins were 92.66, 95.73, and 96.43 %, respectively. The RPD values were all high (> 3), indicating good predictive accuracy. This study suggested that FT-IR could be a tool for the simultaneous measurement of microalgae composition of biochemical contents in microalgae cells.


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Author Biography

Kwanchayanawish MACHANA, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131, Thailand

Faculty of Pharmaceutical Science, Burapha university


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How to Cite

MACHANA, K. ., KANOKRUNG, A. ., SRICHAN, S. ., VONGSAK, B. ., KUTAKO, M. ., & SIAFHA, E. . (2018). Monitoring of Biochemical Compounds and Fatty Acid in Marine Microalgae from the East Coast of Thailand. Walailak Journal of Science and Technology (WJST), 17(4), 334–347.