A Pilot Study on Antimalarial Effects of Moringa oleifera Leaf Extract in Plasmodium berghei Infection in Mice

Somrudee NAKINCHAT, Voravuth SOMSAK

Abstract


The emergence and spread of antimalarial drug resistance of Plasmodium parasites, as well as hypoglycemia, during malaria infection, and subsequent death, are critical problems in malaria-endemic areas. Hence, finding new compounds, especially plant extracts having antimalarial and anti-hypoglycemic activities, are urgently needed. The present study aimed to investigate the antimalarial and anti-hypoglycemic effects of Moringa oleifera leaf extract in Plasmodium berghei infection in mice. Aqueous crude extract of M. oleifera leaves was freshly prepared and used for an efficacy test in vivo. Groups of ICR mice (5 mice in each) were infected with 1´107 infected red blood cells of P. berghei ANKA by intraperitoneal injection and given the extract orally with doses of 100, 500, and 1000 mg/kg for 4 consecutive days. Parasitemia and plasma glucose levels were subsequently measured. The results showed that M. oleifera leaf extract presented significant (p < 0.001) inhibition of parasitemia in a dose-dependent manner. Moreover, this extract exerted anti-hypoglycemia effects in infected mice in a dose-dependent manner. The highest degrees of activity were found at a dose of 1000 mg/kg of the extract. Additionally, no effect on plasma glucose was found in normal mice treated with this extract. It can be concluded that aqueous crude extract of M. oleifera leaves exerted antimalarial and anti-hypoglycemic effects in P. berghei infection in mice.


Keywords


Antimalarial, anti-hypoglycemic, Moringa oleifera, Plasmodium berghei

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References


PJ Hotez, ME Bottazzi, U Strych, LY Chang, YA Lim, MM Goodenow and S AbuBakar. Neglected tropical diseases among the Association of Southeast Asian Nations (ASEAN): Overview and update. PLoS Negl. Trop. Dis. 2015; 9, e0003575.

HA Antony and SC Parija. Antimalarial drug resistance: An overview. Trop. Parasitol. 2016; 6, 30-41.

AA Ali, EM Elhassan, MM Magzoub, MI Elbashir and I Adam. Hypoglycaemia and severe Plasmodium falciparum malaria among pregnant Sudanese women in an area characterized by unstable malaria transmission. Parasit. Vector. 2011; 4, 88.

F Mojab. Antimalarial natural products: A review. Avicenna J. Phytomed. 2012; 2, 52-62.

R Duffy, C Wade and R Chang. Discovery of anticancer drugs from antimalarial natural products: A MEDLINE literature review. Drug. Discov. Today 2012; 17, 942-53.

S Hussain, F Malik and S Mahmood. Review: An exposition of medicinal preponderance of Moringa oleifera (Lank.). Pak. J. Pharm. Sci. 2014; 27, 397-403.

AFA Razis, MD Ibrahim and SB Kntayya. Health benefits of Moringa oleifera. Asian Pac. J. Cancer Prev. 2014; 15, 8571-6.

K Prabhu, K Murugan, A Nareshkumar, N Ramasubramanian and S Bragadeeswaran. Larvicidal and repellent potential of Moringa oleifera against malarial vector, Anopheles stephensi Liston (Insecta: Diptera: Culicidae). Asian Pac. J. Trop. Biomed. 2011; 1, 124-9.

SJ Stohs and MJ Hartman. Review of the safety and efficacy of Moringa oleifera. Phytother Res. 2015; 29, 796-804.

C Rodriguez-Perez, B Gilbert-Lopez, JA Mendiola, R Quirantes-Pine, A Segura-Carretero and E Ibanez. Optimization of microwave-assisted extraction and pressurized liquid extraction of phenolic compounds from Moringa oleifera leaves by multiresponse surface methodology. Electrophoresis 2016; 37, 1938-46.

W Peters. The chemotherapy of rodent malaria, XXII. The value of drug-resistant strains of Plasmodium berghei in screening for blood schizontocidal activity. Ann. Trop. Med. Parasitol. 1975; 69, 155-71.

V Offeddu, M Rauch, O Silvie and K Matuschewski. The Plasmodium protein P113 supports efficient sporozoite to liver stage conversion in vivo. Mol. Biochem. Parasitol. 2014; 193, 101-9.

K Slavic, U Straschil, L Reininger, C Doerig, C Morin, R Tewari and S Krishna. Life cycle studies of the hexose transporter of Plasmodium species and genetic validation of their essentiality. Mol. Microbiol. 2010; 75, 1402-13.

KM Elased, J Taverne and JH Playfair. Malaria, blood glucose, and the role of tumour necrosis factor (TNF) in mice. Clin. Exp. Immunol. 1996; 105, 443-9.

D Jaiswal, PK Rai, S Mehta, S Chatterji, S Shukla, DK Rai, G Sharma, B Sharma, S Khair and G Watal. Role of Moringa oleifera in regulation of diabetes-induced oxidative stress. Asian Pac. J. Trop. Med. 2013; 6, 426-32.

LA Olayaki, JE Irekpita, MT Yakubu and OO Ojo. Methanolic extract of Moringa oleifera leaves improves glucose tolerance, glycogen synthesis and lipid metabolism in alloxan-induced diabetic rats. J. Basic Clin. Physiol. Pharmacol. 2015; 26, 585-93.

PB Memvanga, GL Tona, GK Mesia, MM Lusakibanza and RK Cimanga. Antimalarial activity of medicinal plants from the Democratic Republic of Congo: A review. J. Ethnopharmacol. 2015; 169, 76-98.

IC Ezenyi, OA Salawu, R Kulkarni and M Emeje. Antiplasmodial activity-aided isolation and identification of quercetin-4'-methyl ether in Chromolaena odorata leaf fraction with high activity against chloroquine-resistant Plasmodium falciparum. Parasitol. Res. 2014; 113, 4415-22.

MI Barliana, EW Suradji, R Abdulah, A Diantini, T Hatabu, J Nakajima-Shimada, A Subarnas and H Koyama. Antiplasmodial properties of kaempferol-3-O-rhamnoside isolated from the leaves of Schima wallichii against chloroquine-resistant Plasmodium falciparum. Biomed. Rep. 2014; 2, 579-83.


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