Protective Effects of Thunbergia laurifolia (Linn.) on Organophosphorous (chlorpyrifos)-Induced Cholinesterase Dysfunction

Malatee TAYEH; Jirapat NAWARAT; Moe Pwint PHYU; Jitbanjong TANGPONG

doi:10.48048/wjst.2018.4291

Authors

Malatee TAYEH Division of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161
Jirapat NAWARAT Division of Physical Therapy, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161
Moe Pwint PHYU Division of Biomedical Sciences, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161
Jitbanjong TANGPONG Division of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161

DOI:

https://doi.org/10.48048/wjst.2018.4291

Keywords:

Organophosphorous (chlorpyrifos), cholinesterase (ChE), Thumbergia laurifolia (Linn) (TL), free radical scavenging

Abstract

Orgnophosphorous (chyloryrifos) has been widely used for pest control. It is considered to be functionally irreversible inhibitor of cholinesterase (ChE), since the time necessary to liberate the enzyme from inhibition may be in excess of time required for the synthesis of new ChE. This study was designed to investigate the effects of total phenolic content, total antioxidant capacity and free radical scavenging activities in cell free system present in Thunbergia laurifolia (Linn.) (TL) and further examined for their ability against chloripyrifos induced ChE dysfunction in vitro (blood and brain of mice) studies. The results showed that TL has the highest phenolic content, which is significant positive correlation with its antioxidant activity (R2=0.99). Besides, the fifty percent of inhibitory concentration of TL on scavenging free radical activities (IC₅₀) for hydroxyl, superoxide and hydrogen peroxide radical in cell free system were 1.47±0.02, 2.23±0.08 and 1.66±0.09 mg/mL, respectively. We found TL showed a potential inhibition of chloripyrifos-induced ChE dysfunction in a concentration dependent manner. These data suggest that TL exhibits an anti-oxidant and at least in part neuroprotective properties against chloripyrifos-induced ChE alteration. We strongly believe that these antioxidant constituents are prospective novel nutriceuticals.

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Author Biography

Jitbanjong TANGPONG, Division of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161

SCHOOL OF ALLIED HEALTH SCIENCES AND PUBLIC HEALTH

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