Anti-HIV-1 Protease Activity of Compounds from Cassia garrettiana


  • Kingkan BUNLUEPUECH Faculty of Traditional Thai Medicine, Prince of Songkla University, Songkhla 90112
  • Supinya TEWTRAKUL Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90112
  • Chatchai WATTANAPIROMSAKUL Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90112


Anti-HIV-1 protease activity, longevity preparations, Cassia garrettiana, Thai plants


For the purpose of discovering anti-HIV-1 agents from Thai plant preparations with a history of use in Thai traditional medicine as agents assisting health longevity, aqueous and ethanol extracts from 24 Thai plants were screened for their inhibitory activities against HIV-1 protease (PR) using an anti-HIV-1 PR assay. Five extracts (10 %), 3 ethanol and 2 water, from only 4 plants, Cassia garrettiana, Dioscorea bulbifera, Albizia procera, and Areca catechu, produced extracts with IC50 values of < 45 µg/ml. Thirty five extracts (90 %) had no detectable activity, with IC50 values >100 µg/ml. The ethanol extracts of Cassia garrettiana (heartwood), Areca catechu, and Dioscorea bulbifera were the most potent, with IC50 values of 15.6, 24.9, and 44.4 µg/ml, respectively. For the water extracts, the most potent activity was found in Albizia procera (bark) and Cassia garrettiana (heartwood), with IC50 values of 22.6 and 26.8 µg/ml, respectively. Pure compounds from ethanol extracts of Cassia garrettiana (heartwood) were separated to give 5 compounds, chrysophanol (1), piceatannol (2), aloe-emodin (3), emodin (4), and cassigarol E (5). Of the tested samples, piceatannol (2) showed the highest anti-HIV-1 PR activity (IC50 = 48.29 µM), whereas the % inhibition (mean±S.E.M., n=3) produced by 100 µM of cassigarol E (5), aloe-emodin (3), chrysophanol (1), and emodin (4) were only 27.64±3.8, 27.20±0.7, 26.66±0.6, and 4.52±1.9, respectively.


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How to Cite

BUNLUEPUECH, K., TEWTRAKUL, S., & WATTANAPIROMSAKUL, C. (2016). Anti-HIV-1 Protease Activity of Compounds from Cassia garrettiana. Walailak Journal of Science and Technology (WJST), 13(10), 827–835. Retrieved from