Lipid-Lowering Effects of Hexane Fraction of Ivy Gourd (Coccinia grandis L. Voigt) Root in Mice Fed a High-Fat Diet

Ruthaiwan BUNKRONGCHEAP; Masashi INAFUKU; Hirosuke OKU; Nongporn HUTADILOK-TOWATANA; Chatchai WATTANAPIROMSAKUL; Decha SERMWITTAYAWONG

Authors

Ruthaiwan BUNKRONGCHEAP Department of Biochemistry, Faculty of Science, Prince of Songkla University, Songkhla 90112
Masashi INAFUKU Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Okinawa 903-0213
Hirosuke OKU Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Okinawa 903-0213
Nongporn HUTADILOK-TOWATANA College of Oriental Medicine, Rangsit University, Pathum Thani 12000
Chatchai WATTANAPIROMSAKUL Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90112
Decha SERMWITTAYAWONG Department of Biochemistry, Faculty of Science, Prince of Songkla University, Songkhla 90112

Keywords:

Anti-obesity, ivy gourd root, high-fat fed mice, anti-hyperlipidemia, plasma lipids

Abstract

Ivy gourd is an edible plant widely grown in the tropics. Its root has long been touted to possess anti-obesity property. In our previous study, the ethanolic extract of ivy gourd root exhibited anti-obesity action by potently inhibiting 3T3-L1 preadipocyte differentiation. Bioactivity-guided fractionation of the same extract also revealed that its active principles are in the hexane fraction. Here, we investigate the anti-obesity effects of the hexane fraction of ivy gourd root extract (IGH) in high-fat diet (HFD) induced obese mice and provide evidence of its underlying molecular mechanisms. C57BL/6J mice were fed HFD in the presence or absence of 2 % (w/w) dietary concentration of IGH for 4 weeks. Biochemical determinants of obesity were then measured in these animals. Consumption of IGH caused a decrease of serum triglycerides (TG) and non-esterified fatty acid concentrations as well as hepatic TG and total cholesterol (TC) levels. An increase in fecal excretion of TG and TC along with a decrease in activity of hepatic lipogenesis-related enzymes including fatty acid synthase, glucose-6-phosphate dehydrogenase and malic enzyme in the liver was also detected upon the intake of IGH. These results suggest that IGH may have the potential as an anti-hyperlipidemic agent for obesity prevention and/or management.

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