Estimation of Rumen Undegradable Protein with In Situ Nylon Bag and In Vitro Enzymatic Technique in Tropical Concentrate Feedstuffs


  • Songsak CHUMPAWADEE Department of Agricultural Production Technology, Faculty of Technology, Maha Sarakham University, Maha Sarakham 44000
  • Kritapon SOMMART Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002
  • Thevin VONGPRALUB Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002
  • Virote PATTARAJINDA Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002


Rumen undegradable protein, Nylon bag technique, Enzymatic technique, Concentrate feedstuffs


Seventeen concentrate feedstuffs were used to study the relationships between the in vitro enzymatic technique and the in situ nylon bag technique for rumen undegradable protein determination. Feedstuffs were divided into 6 groups, 1) energy feed, 2) all protein feed, 3) feed higher than 15% crude protein (CP), 4) feed higher than 20% CP 5) feed lower than 20% CP and 6) all test feed. It was found that all test feed had the lowest relationship (R2 = 0.16, P < 0.1, n = 17). On the other hand, the group of higher than 15% CP feed had the highest relationship (R2 = 0.79, P < 0.0002, n = 9). The coefficient of determination (R2) of the energy feed, all protein feed, higher than 20% CP feed and lower than 20% CP feed were 0.66, 0.73, 0.74 and 0.58, respectively. This result suggested that the enzymatic technique can be used to predict the in situ nylon bag technique when the group of feed was separated by protein levels. Therefore, the enzymatic technique is an alternative technique for rumen undegradable protein determination in tropical concentrate feedstuffs, particularly those with high protein feed (>15%CP).


Download data is not yet available.


Metrics Loading ...


Kopecny J Vencl B Kyselova J Brezina P. Determination of rumen degradable protein with enzymes. Arch Anim Nutr 1989; 39: 549-55.

Ørskov ER McDonald I. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J Agric Sci (Camb) 1979; 92: 499-504.

Susmel P Mills CR Colitti M Stefanon B. In vitro solubility and degradability of nitrogen in concentrate ruminant feed. Anim Feed Sci Tech 1993; 42:1-13.

Roe MB Sniffen CJ Chase LE. Techniques for measuring protein fractions in feedstuffs. Proceedings of Cornell Nutrition Conference for Feed Manufactures.

Department of Animal Science and Poultry and Avian Science, New York State Collage of Agriculture and Life Sciences Cornell University. Ithaca, New York. 1990.

Mathis CP Cochran RC Vanzant ES Abdelgadir IEO Heldt JS Olson KC Johnson DE Caton J Faulkner D Horn G Paisley S Mass RK Moore HJ. A collaborative study comparing an in situ protocol with single time point enzyme assays for estimating ruminal protein degradability of different forages. Anim Feed Sci Tech 2001; 93: 31-42.

Cone JW Kamman AA Van Gelder AH Hindle VA. Rumen escape protein in concentrate ingredients determined with the nylon bag and enzymatic techniques. Anim Feed Sci Tech 2002; 97: 247-54.

Krishnamoorthy U Sniffen CJ Stern MD Van Soest PJ. Evaluation of athematical model of rumen digestion and an in vitro simulation of rumen proteolysis to estimate the rumen undegraded nitrogen content of feedstuffs. Br J Nutri 1983; 50: 555-68.

Poos-Floyd M Klopfenstein T Britton RA. Evaluation laboratory techniques for predicting ruminant protein degradation. J Dairy Sci 1985; 68: 829-39.

Tomankova O Kopecny J. Prediction of feed protein degradation in rumen with bromelain. Anim Feed Sci Tech 1995; 53: 71-80.

Mahadevan S Sauer FD Erfle JD. Preparation of protease from mixed rumen microorganisms and its use for the in vitro determination of the degradability of true protein in feedstuffs. Can J Anim Sci 1987; 67: 55-64.

Assoumani MB Vedeau F Jaquot L Sniffen CJ. Refinement of enzymatic method for estimation the theoretical degradability of proteins in feedstuffs for ruminants. Anim Feed Sci Tech 1992; 39: 358-68.

AOAC. Official Methods of Analysis. 15th Edition. Association of Official Analytical Chemists Arlington Virginia U.S.A. 1990.

Van Soest PJ Robertson JB Lewis BA. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991; 74: 3583-97.

Shabi Z Arieli A Bruckental L Aharoni Y Zamwel S Bor A Tagari H. 1998. Effect of the synchronization of the degradation of dietary crude protein and organic matter and feeding frequency on ruminal fermentation and flow of digesta in the abomasum of dietary cows. J Dairy Sci 1998; 81: 1991-2000.

SAS User’s Guide: Statistics, Version 6. 12th Edition. SAS Institute Inc. Cary, NC. 1996.

von Keyserlingk MAG Swift ML Puchala R Shelford V. Degradability characteristics of dry matter and crude protein of forages in ruminants. Anim Feed Sci Tech 1996; 57: 291-311.

Mupangwa JF Ngongoni NT Topps JH Ndlovu P. Chemical composition and dry matter of forage legumes Cassia rotundiforlia cv. Wynn, Lablab purpureus cv. Highworth and Macroptilium atropurpureum cv. Siratro at 8 weeks of growth (preanthesis). Anim Feed Sci Tech 1997; 69: 167-78.

Mara FPO Mulligan FJ Cronin EJ Rath M Caffrey PJ. The nutritive value of palm kernel meal measured in vivo and using rumen fluid and enzymatic techniques. Livest Prod Sci 1999; 60: 305-16.

Thu NV Preston TR. Rumen environment and feed degradability in swamp buffaloes fed different supplements. Livest Res Rural Devel 1999; 11: 1-7.

Roe MB Chase LE Sniffen CJ. Comparison of in vitro technique to the in situ technique for estimation of ruminal degradation of protein. J Dairy Sci 1991; 74:1632-40.

Licitra G Van Soest PJ Schadt I Carpino S Sniffen CJ. Influence of the concentration of the protease from Streptomysis griseus relative to ruminal protein degradability. Anim Feed Sci Tech 1999; 77: 99-113.

Kopecny J Tomankova O Homolka P. Comparison of protein digestibility of rumen undegradable protein estimated by an enzymatic and mobile bag method; feed for ruminants and anaerobic fungus. Anim Feed Sci Tech 1998; 71: 109-16.

Shannak K Sudekum H Susenbeth A. Estimating ruminal crude protein degradation with in situ and chemical fraction procedures. Anim Feed Sci Tech 2000; 85:195-214.




How to Cite

CHUMPAWADEE, S., SOMMART, K., VONGPRALUB, T., & PATTARAJINDA, V. (2011). Estimation of Rumen Undegradable Protein with In Situ Nylon Bag and In Vitro Enzymatic Technique in Tropical Concentrate Feedstuffs. Walailak Journal of Science and Technology (WJST), 2(1), 23–33. Retrieved from



Research Article