Genetic Diversity in Slender Walking Catfish (Clarias nieuhofii) Populations: Implications for Population Management


  • Jamjun PECHSIRI Aquaculture Biotechnology Research Unit, Faculty of Science, Thaksin University Phatthalung Campus, Phatthalung 93110
  • Arunrut VANICHANON Aquaculture Biotechnology Research Unit, Faculty of Science, Thaksin University Phatthalung Campus, Phatthalung 93110


Clarias nieuhofii, vulnerable species, population structure, genetic diversity, RAPD


Random amplified polymorphic DNA (RAPD) markers were applied to assess the genetic diversity and population structure of the slender walking catfish (Clarias nieuhofii, Clariidae) from 3 wild populations in peat swamp forests in southern Thailand (Surat Thani, Narathiwat, and Phatthalung). The selected 14 RAPD primers produced 105 RAPD bands, ranging from 6 to 11 bands per primer, and ranging in size between 400 and 3,000 bp. The percentage of polymorphic loci, gene diversity and Shannon’s information index values were 75.00 %, 0.2252, and 0.3443 for Surat Thani; 86.59 %, 0.2982, and 0.4441 for Narathiwat, and 96.25 %, 0.3371, and 0.5049 for Phatthalung, respectively. Among the 3 populations, the highest genetic distance (0.2213) was found between the Narathiwat and Surat Thani populations. High genetic differentiation (Gst = 0.2815) was detected in 3 populations with low gene flow (Nm = 1.2762) among the overall populations. The clustering pattern obtained by the unweighted pair-group (UPGMA) method separated the C. nieuhofii samples into 3 groups, but 2 clusters. The results indicated a high level of genetic variation and genetic differentiation among C. nieuhofii from different populations in southern Thailand. This information would be useful to construct appropriate breeding programs, and could help conserve populations used as potential sources for stock management, restocking programs, and sustainable uses.


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Author Biography

Jamjun PECHSIRI, Aquaculture Biotechnology Research Unit, Faculty of Science, Thaksin University Phatthalung Campus, Phatthalung 93110

Faculty of Science, Asst. Prof.


C Vidthayanon. Thailand Red Data: Fishes. Office of Natural Resources and Environmental Policy and Planning (ONEP), Thailand, 2005, p. 1-81.

M Danish, IJ Singh, P Giri and CP Singh. Molecular characterization of two populations of catfish Clarias batrachus L. using random amplified polymorphic DNA (RAPD) markers. Afr. J. Biotechnol. 2012; 11, 14217-26.

AT Norris, DG Bradley and EP Cunningham. Microsatellite genetic variation between and with farmed and wild Atlantic salmon (Salmo salar) populations. Aquaculture 1999; 180, 247-64.

JC Pereira, PG Lino, A Leitao, S Joaquim, R Chaves, P Pousao-Ferreira, H Guedes-Pinto and MN dos Santos. Genetic differences between wild and hatchery populations of Diplodus sargus and D. vulgaris inferred from RAPD markers: Implications for production and restocking programs design. J. Appl. Genet. 2010; 51, 67-72.

H Hadrys, M Balick and B Schierwater. Applications of random amplified polymorphic DNA (RAPD). Mol. Ecol. 1992; 1, 55-63.

PMA Muneer, A Gopalakrishnan, KK Musammilu, V Mohindra, KK Lal, VS Basheer and WS Lakra. Genetic variation and population structure of endemic yellow catfish, Horabagrus brachysoma (Bagridae) among three populations of Western Ghat region using RAPD and microsatellite markers. Mol. Biol. Rep. 2009; 36, 1779-91.

RK Garg, P Sairkar, N Silawat, N Vijay, N Batav and NN Mehrotra. Genetic diversity between two populations of Heteropneustes fossilis (Bloch) using RAPD profile. Int. J. Zool. Res. 2009; 5, 171-7.

RK Garg, N Silawat, P Sairkar, N Vijay and NN Mehrotra. RAPD analysis for genetic diversity of two populations of Mystus vittatus (Bloch) of Madhya Pradesh, India. Afr. J. Biotechnol. 2009; 8, 4032-8.

S Sultana, S Akter, MAR Hossain and MS Alam. DNA fingerprinting of the Asian stinging catfish (Heteropneustes fossilis, Bloch) by Random Amplified Polymorphic DNA markers. Int. J. Biotechnol. Appl. 2010; 2, 5-10.

RK Garg, P Sairkar, N Silawat, N Batav and NN Mehrotra. Assessment of genetic diversity of Clarias batrachus using RAPD markers in three water bodies of Bhopal. J. Environ. Biol. 2010; 31, 749-53.

GD Khedkar, ACS Reddy, M Persis, R Kondadhasula and K Muzumdar. Clarias batrachus (Linn.1758) population is lacking genetic diversity in India. Mol. Biol. Rep. 2010; 37, 1355-62.

PMA Muneer, R Sivanandan, A Gopalakrishnan, VS Basheer, KK Musammilu and AG Ponniah. Development and characterization of RAPD and microsatellite markers for genetic variation analysis in the critically endangered yellow catfish Horabagrus nigricollaris (Teleostei: Horabagridae). Biochem. Genet. 2011; 49, 83-95.

S Kumla, S Doolgindachbaporn, R Sudmoon and N Sattayasai. Genetic variation, population structure and identification of yellow catfish, Mystus nemurus (C&V) in Thailand using RAPD, ISSR and SCAR marker. Mol. Biol. Rep. 2012; 39, 5201-10.

J Rashid, FM Tamanna, MAR Hossain, and S Alam. Genetic variation in endangered butter catfish, Ompok bimaculatus (Bloch) populations revealed by random amplified polymorphic DNA (RAPD) fingerprinting. Int. J. Biosci. 2012; 2, 85-93.

FM Tamanna, J Rashid and S Alam. High levels of genetic variation revealed in wild populations of the stripped dwarf catfish Mystus vittatus (Bloch) (Bagridae: Siluriformes) in Bangladesh by random amplified polymorphic DNA techniques. Int. J. Adv. Biotec. Res. 2012; 2, 322-7.

JB Taggart, RA Hynes, PA Prodohl and A Ferguson. A simplified protocol for routine total DNA isolation from salmonid fishes. J. Fish Biol. 1992; 40, 963-5.

FC Yeh, RC Yang and T Boyle. POPGENE 32 - Version 1.31. Population Genetics Software, Available at:, Accessed June 2014.

M Nei. Genetic distance between populations. Amer. Nat. 1972; 106, 283-92.

YM Saad, MS Hanafi, MA Essa, AA Guerges and SF Ali. Genetic signatures of some Egyptian Clarias gariepinus populations. Glob. Veteri. 2009; 3, 503-8.

S Wright. Evolution and the Genetics of Populations: Vol. 4, Variability within and among Natural Populations. University of Chicago Press, Chicago, 1978. p. 83-107.

MN Islam, MdS Islam and MdS Islam. Genetic structure of different populations of walking catfish (Clarias batrachus L.) in Bangladesh. Biochem. Genet. 2007; 45, 647-62.

G Chandra, A Saxena and A Barat. Genetic diversity of two riverine populations of Eutropiichthys vacha (Hamilton, 1822) using RAPD markers and implications for its conservation. J. Cell Mol. Biol. 2010; 8, 77-85.




How to Cite

PECHSIRI, J., & VANICHANON, A. (2015). Genetic Diversity in Slender Walking Catfish (Clarias nieuhofii) Populations: Implications for Population Management. Walailak Journal of Science and Technology (WJST), 13(7), 511–519. Retrieved from



Research Article

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