Decontamination of Monodon Baculovirus in Marine Shrimp Eggs using Upwelling Flow-Through System



Penaeus monodon-type baculovirus (MBV) is a horizontal transmission virus disease especially found in marine shrimp. Even though sanitization of MBV contamination in shrimp hatcheries by using clean seawater or chemicals has been recommended, the prevalence of the disease in larvae is still frequently reported. This study is carried out in an attempt to improve previous sanitization methods by using the upwelling flow-through vessel developed for this study. Eggs of Penaeus monodon were obtained from infected broodstocks collected from the wild. Decontamination of MBV was performed using three methods. Treatment 1: The eggs were continuously rinsed with chlorinated seawater for 1 min and transferred to a nursing tank. The eggs were allowed to hatch and reared to the postlarval stage in a nursing tank. Treatment 2: The eggs were rinsed with chlorinated seawater for 1 min and hatched in the upwelling vessel. Following hatching, the nauplius larvae were reared to the postlarval stage in a nursing tank. Treatment 3: The eggs were bathed with 20 mg/l iodophore for 30 s and hatched in the upwelling vessel. Following hatching, the nauplius larvae were reared to the postlarval stage in a nursing tank. There was no MBV observed in shrimp at the nauplius stage in all treatments. However, at day 11, when larvae developed to postlarva 1, the infection was found in the shrimp of treatment 1. At day 15 (postlarva 5), MBV was detected in all treatments. At day 20 (postlarva 10), viral incidences were observed in all treatments. This concludes that the upwelling flow-through system developed in this study is able to delay the occurrence of MBV in shrimp larvae. However, it is most likely that horizontal transmission of MBV in P. monodon is associated with other means for which the eradication processes, including iodophore bathing and egg cleansing, provide insufficient protection against.



Decontamination, Monodon Baculovirus, MBV, shrimp egg, upwelling flow-through system

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DV Lightner and RM Redman. A bacurovirus-caused disease of the penaeid shrimp, Penaeus monodon. J. Invertebr. Pathol. 1981; 38, 299-302.

TW Flegel. An Overview of Prawn Viral Disease Work in Thailand: PCR for Shrimp Disease Diagnosis. Manual of Molecular Diagnostics for Shrimp Viruses in Asian Region: ACIRA-NSTDA (BIOTEC)-Crawford Fund; 1999.

W Surachetpong, BT Poulos, KFJ T and DV Lightner. Improvement of PCR method for the detection of monodon baculovirus (MBV) in penaeid shrimp. Aquaculture 2005; 249, 69-75.

DV Lightner, RM Redman and TA Bell. Observations on the geographic distribution, pathogenesis and morphology of the baculovirus from Penaeus monodon Fabricius. Aquaculture 1983; 32, 209-33.

IC Liao and Y-H Chien. The evolution of the grass prawn (Penaeus monodon) hatchery industry in Taiwan. Aquacul. Eng. 1996; 15, 111-31.

PC Loh, LM Tapay, Y Lu and ECB Jr Nadala. Viral Pathogens of the Penaeid Shrimp. In: FAMK Maramorosch and JS Aaron (eds.). Advances in Virus Research, Academic Press, 1997, p. 263-312.

SN Chen, PS Chang and GH Kou. Infection route and eradication of Penaeus monodon Baculovirus (MBV) in larval giant tiger prawn, Penaeus monodon. Diseases of Cultured Penaeid Shrimp in Asia and the United States, The Oceanic Institute, Honolulu, Hawaii, 1992.

P Chotipuntu, S Wuthisuthimethawee, S Direkbusrakom and S Songtuay. Reproductive aspects of SPF Penaeus monodon grown in closed culture captivity. Walailak J. Sci. & Tech. 2013; 10, 227-36.

S Wuthisuthimethavee, P Lumubol, S Tragoonrung and A Vanavichit. Development of microsatellite markers and their applications in black tiger shrimp (Penaeus monodon Fabricius) breeding programs. In: Proceeding of the 1st National Symposium on Marine Shrimp, Songkhla, Thailand, 1999.

P Pongtippatee-Taweepreda, J Chavadej, P Plodpai, B Pratoomchart, P Sobhon, W Weerachatyanukul and B Withyachumnarnkul. Egg activation in the black tiger shrimp Penaeus monodon. Aquaculture 2004; 234, 183-98.

FAO. Health management and biosecurity maintenance in white shrimp (Penaeus vannamei) hatcheries in Latin America. FAO Fisheries Technical Paper No 450, 2003.

JL Paynter, JE Vickers and RJG Lester. Experimental transmission of Penaeus monodon-type baculovirus (MBV). In: M Shariff, RP Subasinghe and JR Arthu (eds.). Diseases in Asian Aquaculture, Asian Fisheries Society, Manila, 1992, p. 97-100.

M Sarathi, G Balasubramanian, VK Sivakumar and ASS Hameed. Artemia is not a vector for monodon baculovirus (MBV) transmission to Penaeus monodon. J. Fish Dis. 2008; 31, 631-6.

G Sankar, K Hemalatha, K Ramamorthy and K Sakkaravarthi. Surveillance of monodon baculo virus transmission among shrimpsin wild and laboratory environment. Ann. Biol. Res. 2011; 2, 388-93.


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