Isolation of Antagonistic Actinomycetes Species from Rhizosphere as Effective Biocontrol Against Oil Palm Fungal Diseases
Keywords:Antagonist, biological control, Elaeis guineensis, fungal diseases
Antagonistic actinomycetes were isolated from oil palm rhizosphere and tested against oil palm fungal diseases caused by Curvularia oryzae PSU-NK1012, Ganoderma boninense T1, and Schizophyllum commune SK1, that are the causal agents of leaf spot, stem rot, and brown germ diseases, respectively. Three isolates of actinomycetes were selected for inhibition tests against these fungal pathogens. Based on morphological, physiological, biochemical, and molecular properties, the 3 actinomycetes species were identified as Streptomyces abikoensis ChM3-1, Kitasatospora nipponensis KM6-4, and S. angustmyceticus NR8-2, time course of in vitro tests showed that these actinomycetes species were capable of inhibiting the growth of oil palm pathogenic fungi. Scanning electron microscope observations revealed that the actinomycetes culture filtrates could degrade the mycelia cell walls of the 3 disease pathogens, possibly due to the production of some antimicrobial agents. The study identified an actinomycetes strain with antagonist activity that could be used for biocontrol of plant pathogenic fungi.
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