Isolation and Screening of Potential Lignocellulolytic Microorganisms from Rubber Bark and Other Agricultural Residues

Authors

  • Phat SAKPETCH Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112
  • Aran H-KITTIKUN Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112
  • Ausa CHANDUMPAI Department of Biochemistry, Faculty of Science, Prince of Songkla University, Songkhla 90112

Keywords:

Cellulase, compost, ligninase, lignocellulolytic microorganisms, rubber bark

Abstract

Lignocellulolytic microorganisms were isolated from cow manure, the soil from naturally grown bamboo, the piles of rubber bark and the composting piles. The samples were collected at 9 different locations which were 5 cm deep from the surface. The basal medium (BM) containing CMC (carboxymethyl cellulose) or xylan as a carbon source and the lignin modifying basal medium (LBM) with ABTS {2,2¢-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid} or DMP (2,6-dimethoxyphenol) as an inducer were used for the primary screening of microorganisms producing cellulase, xylanase, laccase and manganese peroxidase, respectively. Forty-five bacterial isolates (B), twenty-five isolates of actinomyces (A) and fifteen fungal isolates (F) were obtained. After that twelve isolates were selected to evaluate enzyme production in LBM containing decomposed rubber bark as a carbon source at 30 °C and at 45 °C under aerobic conditions (150 rpm shaking). The isolates F6, A2 and B15 displayed the highest enzyme activities with a CMCase activity of 0.61, 0.33 and 0.21 U/mL, respectively. Moreover, they also produced xylanase with an activity of 0.61, 0.36 and 0.32 U/mL, respectively. However, only the isolate F6 showed laccase activity and manganese peroxidase activity of 0.81 and 0.56 U/mL, respectively. The isolates B15 and A2 were identified by 16S rDNA with 99 % similarity to Bacillus subtilis and Streptomyces thermovulgaris, respectively. Aside from that, isolate F6 was identified by 18S rDNA with 100 % similarity to Trichoderma asperellum. When these microorganisms were grown in the LBM broth with 10 % decomposed rubber bark they produced the highest lignocellulolytic enzymes in the stationary phase of growth.

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References

P Krukanont and S Prasertsan. Geographical distribution of biomass and potential sites of rubber wood fired power plants in Southern Thailand. Biomass Bioenerg. 2004; 26, 47-59.

S Kaewluan and S Pipatmanomai. Gasification of high moisture rubber wood chip with rubber waste in a bubbling fluidized bed. Fuel Process Tech. 2011; 92, 671-7.

P Chow, FS Nakayama, B Blahnik, JA Youngquist and TA Coffelt. Chemical constituents and physical properties of guayule wood and bark. Ind. Crop. Prod. 2008; 28, 303-8.

H Kausar, M Sariah, HM Saud, MZ Alam and MR Ismail. Development of compatible lignocellulolytic fungal consortium for rapid composting of rice straw. Int. Biodeter. Biodegr. 2011; 64, 594-600.

S Santoshkumar, E Sujatha and P Shivakrishna. Production and optimization of exo and endocellulases from therophilic fungi Scytalidium thermophilum SKESMBKU02. Walailak J. Sci. Tech. 2016; 13, 9-22.

S Ghaffari, AA Sepahi, MR Razavi, F Malekzadeh and H Haydarian. Effectiveness of inoculation with isolated Anoxybacillus sp. MGA110 on municipal solid waste composting process. Afr. J. Microbiol. Res. 2011; 5, 5373-8.

FM Rashad, WD Saleh and MA Moselhy. Bioconversion of rice straw and certain agro-industrial wastes to amendments for organic farming systems: 1. Composting, quality, stability and maturity indices. Bioresource Tech. 2010; 101, 5952-60.

N Sharma, P Buragohain, D Tandon and R Kaushal. Comparative study of potential cellulolytic and xylanolytic bacteria isolated from compost and their optimization for industrial use. J. Agroaliment. Proc. Tech. 2013; 19, 284-97.

L Wang, L Wang, D Wang and J Li. Isolation and application of thermophilic and psychrophilic microorganisms in the composting process. Waste Biomass Valor. 2014; 5, 433-40.

GR Tortella, O Rubilar, L Gianfreda, E Valenzuela and MC Diez. Enzymatic characterization of Chilean native wood-rotting fungi for potential use in the bioremediation of polluted environments with chlorophenols. World J. Microbiol. Biotechnol. 2008; 24, 2805-18.

TK Kirk and M Shimada. Lignin Biodegradation: The Microorganisms Involved and the Physiology and Biochemistry of Degradation by White-rot Fungi. In: T Higuchi (ed.). Biosynthesis and Biodegradation of Wood Components. 1st ed. Academic Press, Orlando, 1985, p. 579-605.

R Omar, A Idris, R Yunus, K Khalid and MI Aida Isma. Characterization of empty fruit bunch for microwave-assisted pyrolysis. Fuel 2011; 90, 1536-44.

Ministry of Agriculture and Cooperative. Thai Agricultural Standard, Compost (in Thai). National Bureau of Agricultural Commodity and Foods Standards, Ministry of Agriculture and Cooperative, Thailand, 2012, p. 1-3.

A Walkley and IA Black. An examination of the Degtijareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 1934; 37, 29-38.

RC Kasana, R Salwan, H Dhar, S Dutt and A Gulati. A rapid and easy method for the detection of microbial cellulases on agar plates using Gram’s Iodine. Curr. Microbiol. 2008; 57, 503-7.

P Reanprayoon and W Pathomsiriwong. Tropical soil fungi producing cellulase and related enzymes in biodegradation. J. Appl. Sci. 2012; 12, 1909-20.

D Peciulyte. Isolation of cellulolytic fungi from waste paper gradual recycling materials. Ekologija 2007; 53, 11-8.

S Sadhu, P Saha, S Mayilra and TK Maiti. Characterization of Bosea sp. strain SF5 (MTCC 10045) isolated from compost soil capable of producing cellulase. J. Microbiol. Biotechnol. Food Sci. 2012; 2, 576-91.

GL Miller. Use of dinitrosalicylic acid reagent for the determination of reducing sugars. Anal.Chem. 1959; 31, 426-8.

JLS Lemos, EPS Bon, MFB Santana and NP Junior. Thermal stability of xylanases produced by Aspergillus awamori. Braz. J. Microbiol. 2000; 31, 206-11.

AA Leontievsky, NM Myasoedova, BP Baskunov, LA Golovleva, C Bucke and CS Evans. Transformation of 2,4,6-trichlorophenol by free and immobilized fungal laccase. Appl. Microbiol. Biotechnol. 2001; 57, 85-91.

A Heinfling, MJ Martínez, AT Martínez, M Bergbauer and U Szewzyk. Purification and characterization of peroxidases from the dye-decolorizing fungus Bjerkandera adusta. FEMS Microbiol. Lett. 1998; 165, 43-50.

LA Hayes and DR Lovley. Specific 16s rDNA sequences associated with naphthalene degradation under sulfate-reducing conditions in harbor sediments. Microb. Ecol. 2002; 43, 134-45.

YT Hoshino and S Morimoto. Comparison of 18S rDNA primers for estimating fungal diversity in agricultural soils using polymerase chain reaction-denaturing gradient gel electrophoresis. Soil Sci. Plant Nutr. 2008; 54, 701-10.

S Singh and L Nain. Microorganisms in the conversion of agricultural wastes to compost. Proc. Indian Nat. Sci. Acad. 2014; 80, 473-81.

T Kaosol, S Kiepukdee and P Towatana. Influence of nitrogen containing wastes addition on natural aerobic composting of rice straw. Am. J. Agri. Biol. Sci. 2012; 7, 121-8.

A Dhouib, M Hamza, H Zouari, T Mechichi, R Hmidi, M Labat, MJ Martínez and S Sayadi. Autochthonous fungal strains with high ligninolytic activities from Tunisian biotopes. Afr. J. Biotechnol. 2005; 4, 431-6.

AS Lakshmi and G Narasimha. Production of cellulases by fungal cultures isolated from forest litter soil. Ann. For. Res. 2012; 55, 85-92.

S Khianngam, A Akaracharanya, W Visessanguan, KK Kim, KC Lee, JS Lee and S Tanasupawat. Diversity of xylanolytic bacteria isolated from Thai sources. Int. J. Biol. 2013; 5, 13-24.

B Kumari, RC Upadhyay and NS Atri. Screening and evaluation of extra-cellular oxidases in some termitophilous and lepiotoid mushrooms. World J. Agri. Sci. 2012; 8, 409-14.

S Samuel, SM Muthukkaruppan, SN Gayatri and KPK Senthil. Cellulase production by Bacillus spp and Aspergillus niger using coir waste and saw dust and partial purification. Int. J. Curr. Res. 2010; 10, 31-4.

SP Gautam, PS Bundela, AK Pandey, Jamaluddin, MK Awasthi and S Sarsaiya. Diversity of cellulolytic microbes and the biodegradation of municipal solid waste by a potential strain. Int. J. Microbiol. 2012; 2012, 325907.

CM Devi and SM Kumar. Isolation and screening of lignocellulose hydrolytic saprophytic fungi from dairy manure soil. Ann. Biol. Res. 2012; 3, 1145-52.

NSC Tabao and RG Monsalud. Screening and optimization of cellulase production of Bacillus strains isolated from Philippine mangroves. Phil J. Syst. Biol. 2010; 4, 79-87.

YK Kim, SC Lee, YY Cho, HJ Oh and YH Ko. Isolation of cellulolytic Bacillus subtilis strains from agricultural environments. ISRN. Microbiol. 2012; 2012, 650563.

J Seo, TS Park, JN Kim, JK Ha and S Seo. Production of endoglucanase, beta-glucosidase and xylanase by Bacillus licheniformis grown on minimal nutrient medium containing agriculture residues. Asian-Australas. J. Anim. Sci. 2014; 27, 946-50.

NEA El-Naggar, NAM Abdelwahed, WIA Saber and AA Mohamed. Bioprocessing of some agro-industrial residues for endoglucanase production by the new subsp.; Streptomyces albogriseolus subsp. cellulolyticus strain NEAE-J. Braz. J. Microbiol. 2014; 45: 743-56.

WJ Chi, JH Lim, DY Park, JS Park and SK Hong. Production and characterization of a thermostable endo-type beta-xylanase produced by a newly-isolated Streptomyces thermocarboxydus subspecies MW8 strain from Jeju Island. Process Biochem. 2013; 48, 1736-43.

S Selvamani, RAb Wahab and F Huyop. A novel putative non-ligninolytic dehalogenase activity for 3-chloropropionic acid (3CP) utilization by Trichoderma asperellum strain SD1. Mal. J. Microbiol. 2015; 11, 265-72.

RA Dar and UG Phutela. Production of thermostable lignolytic enzymes by Thermoascus aurantiacus MTCC 375 using paddy straw as substrate. Int. J. Curr. Microbiol. App. Sci. 2014; 3, 116-21.

JR Monte, W Carvalho and AMF Milagres. Use of a mixture of thermophilic enzymes produced by the fungus Thermoascus aurantiacus to enhance the enzymatic hydrolysis of sugarcane bagasse cellulose. Am. J. Agri. Biol. Sci. 2010; 5, 468-76.

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Published

2017-06-15

How to Cite

SAKPETCH, P., H-KITTIKUN, A., & CHANDUMPAI, A. (2017). Isolation and Screening of Potential Lignocellulolytic Microorganisms from Rubber Bark and Other Agricultural Residues. Walailak Journal of Science and Technology (WJST), 14(12), 953–967. Retrieved from https://wjst.wu.ac.th/index.php/wjst/article/view/2886