Bacteriocin Production by Enterococcus faecalis TS9S17 in MRS Medium with Tuna Condensate as a Nitrogen Source and Its Characteristics
Keywords:
Bacteriocin, lactic acid bacteria, tuna condensate, Enterococcus faecalisAbstract
The purpose of this study was to optimize bacteriocin production by Enterococcus faecalis TS9S17 isolated from a mangrove forest. Ent. faecalils TS9S17 produced 40 AU/ml bacteriocin in MRS medium at an initial pH of 6.5 and at 37 °C for 24 h, which inhibited Lactobacillus sakei subsp. sakei JCM 1157 and Listeria monacytogenes. When 26.5 g/l of tuna condensate was used instead of peptone, beef extract, and yeast extract in the MRS medium, Ent. faecalils TS9S17 also produced 40 AU/ml of bacteriocin. The maximum bacteriocin activity, 160 AU/ml, was obtained at pH 6.5 and at 25 °C. Crude bacteriocin displayed the highest activity at pH 6 and remained active over a wide pH range. It was active after heating at 60 °C for 60 min, but showed little activity after heating at 121 °C for 15 min. It could be kept at -20 °C for more than 4 weeks. This study shows that tuna condensate could be used as a low cost nitrogen source for bacteriocin production.
Downloads
Metrics
References
LT Axelsson. Lactic Acid Bacteria: Classification and Physiology. In: S Salminen and A von Wright (eds.). Lactic Acid Bacteria. 2nd ed. Marcel Dekker, New York, 1993, p. 1-64.
T Aymerich, MG Artigas, M Garriga, JM Monfort and M Hugas. Effect of sausage ingredients and additives on the production of enterocins A and B by Enterococcus faecium CTC492 optimization of in vitro production and anti-listerial effect in dry fermented sausages. J. Appl. Microbiol. 2000; 88, 686-94.
S Ananou, A Munoz, A Galvez, M Martinez-Bueno, M Maqueda and E Valdivia. Optimization of enterocin AS-48 production on a whey-based substrate. Int. Dairy J. 2008; 18, 923-7.
SD Todorov, M Wachsman, E Tomé, X Dousset, MT Destro, LM Dicks, BD Franco, M Vaz-Velho and D Drider. Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium. Food Microbiol. 2010; 27, 869-79.
SD Todorov and LMT Dicks. Comparison of two methods for purification of plantaricin ST31, a bacteriocin produced by Lactobacillus plantarum ST31. Braz. J. Microbiol. 2004; 35, 157-60.
C Herranz, JM Martinez, JM Rodriguez, PE Hernandez and LM Cintas. Optimization of enterocin P production by batch fermentation of Enterococcus faecium P13 at constant pH. Appl. Microbiol. Biotechnol. 2001; 56, 378-83.
MA Yusuf and THTA Hamid. Optimization of temperature and pH for the growth and bacteriocin production of Enterococcus faecium. B3L3. IOSR J. Pharm. 2012; 2, 49-59.
HY Choi, JS Kim and WJ Kim. Optimization of conditions for the maximum bacteriocin production of Enterococcus faecium DB1 using response surface methodology. Korean J. Food Sci. Anim. Resour. 2011; 31, 176-82.
M Mirhosseini and G Emtiazi. Optimisation of enterocin a production on a whey-based substrate. World Appl. Sci. J. 2011; 10, 1493-9.
P Juntraporn. 2000, Optimization for Bacteriocin Production by Immobilized Lactobacillus casei ssp. rhamnosus (SN 11). MSc. Thesis. Prince of Songkla University, Thailand.
N Hwanhlem, JM Chobert and A H-Kittikun. Bacteriocin-producing lactic acid bacteria isolated from mangrove forests in southern Thailand as potential bio-control agents in food: Isolation, screening and optimization. Food Contr. 2014; 41, 202-11.
B Aslim, ZN Yuksekdag, E Sarikaya and Y Beyatli. Determination of the bacteriocin-like substances produce by some lactic acid bacteria isolated from Turkish dairy products. LWT Food Sci. Tech. 2005; 38, 691-4.
U Schilliger and FK Lucke. Antibacterial activity of Lactobacillus sake isolate from meat. Appl. Environ. Microbiol. 1989; 55, 1901-6.
ST Ogunbanwo, AI Sanni and AA Onilude. Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1. Afr. J. Biotech. 2003; 2, 219-27.
AOAC International. Official Methods of Analysis of the Association of Official Chemists International. 16th ed. Gaithersburg, 2012.
JD Man, M Rogosa and ME Sharpe. A medium for the cultivation of lactobacilli. J. Appl. Bact. 1960; 23, 130-5.
OH Lowry, NJ Rosebrough, AL Farr and RJ Randall. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951; 193, 265-75.
MD Toit, CMAP Franz, LMT Dicks and WH Holzapfel. Preliminary characterization of bacteriocins produced by Enterococcus faecium and Enterococcus faecalis isolated from pig faeces. J. Appl. Microbiol. 2000; 88, 482-94.
I Ohkawa, S Shiga and M Kageyama. Effect of iron concentration in the growth medium on the sensitivity of Pseudomonas aeruginosa to pyocin S2. J. Biochem. 1980; 87, 323-31.
L Junudom. 2006, Development of Medium for Cultivation and Purification of Bacteriocin from Lactobacillus casei ssp. rhamnosus SN 11. MSc. Thesis. Prince of Songkla University, Thailand.
LD Vuyst, R Callewaert and K Crabbe. Primary metabolite kinetics of bacteriocins biosynthesis by Lactobacillus amylovorus and evidence for stimulation of bacteriocins production under unfavourable growth conditions. Microbiology 1996; 142, 817-27.
F Leroy and LD Vuyst. Bacteriocin production by Enterococcus faecium RZSC5 is cell density limited and occurs in the very early growth phase. Int. J. Food Microbiol. 2002; 72, 155-64.
T Zendo, N Eungruttanagorn, S Fujioka, Y Tashiro, K Nomura, Y Sera, G Kobayashi, J Nakayama, A Ishizaki and K Sonomoto. Identification and production of a bacteriocin from Enterococcus mundtii QU 2 isolated from soybean. J. Appl. Microbiol. 2005; 99, 1181-90.
A Rehaiem, B Martinez, M Manai and A Rodriguez. Production of enterocin A by Enterococcus faecium MMRA isolated from ‘Rayeb’, a traditional Tunisian dairy beverage. J. Appl. Microbiol. 2010; 108, 1685-93.
N Hwanhlem, V Biscola, S El-Ghaish, E Jaffres, X Dousset, T Haertle, A H-Kittikun and JM Chobert. Bacteriocin-producing lactic acid bacteria isolated from mangrove forests in southern Thailand as potential bio-control agents: Purification and characterization of bacteriocin produced by Lactococcus lactis subsp. lactis KT2W2L. Probiotics Antimicrob. Prot. 2013; 5, 264-78.
B Kaur, P Balgir, B Mittu, A Chauhan and B Kumar. Purification and physicochemical characterization of anti-Gardnerella vaginalis bacteriocin HV6b produced by Lactobacillus fermentum isolate from human vaginal ecosystem. Am. J. Biochem. Mol. Biol. 2013; 3, 91-100.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2016 Walailak Journal of Science and Technology (WJST)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.