Estimation of Rumen Undegradable Protein with In Situ Nylon Bag and In Vitro Enzymatic Technique in Tropical Concentrate Feedstuffs

Songsak CHUMPAWADEE, Kritapon SOMMART, Thevin VONGPRALUB, Virote PATTARAJINDA

Abstract


Seventeen concentrate feedstuffs were used to study the relationships between the in vitro enzymatic technique and the in situ nylon bag technique for rumen undegradable protein determination. Feedstuffs were divided into 6 groups, 1) energy feed, 2) all protein feed, 3) feed higher than 15% crude protein (CP), 4) feed higher than 20% CP 5) feed lower than 20% CP and 6) all test feed. It was found that all test feed had the lowest relationship (R2 = 0.16, P < 0.1, n = 17). On the other hand, the group of higher than 15% CP feed had the highest relationship (R2 = 0.79, P < 0.0002, n = 9). The coefficient of determination (R2) of the energy feed, all protein feed, higher than 20% CP feed and lower than 20% CP feed were 0.66, 0.73, 0.74 and 0.58, respectively. This result suggested that the enzymatic technique can be used to predict the in situ nylon bag technique when the group of feed was separated by protein levels. Therefore, the enzymatic technique is an alternative technique for rumen undegradable protein determination in tropical concentrate feedstuffs, particularly those with high protein feed (>15%CP).

Keywords


Rumen undegradable protein, Nylon bag technique, Enzymatic technique, Concentrate feedstuffs

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