The effect of neem leaves (Azadirachta indica A. Juss) application as a natural disinfectant on decreasing number of bacteria and fungi in poultry incubator

Authors

  • Yuli Astuti HIDAYATI Universitas Padjadjaran, Sumedang 45363
  • Rena YULIA Universitas Padjadjaran, Sumedang 45363
  • Santy ROSITA Universitas Padjadjaran, Sumedang 45363
  • Khairunnisa Nur RAHMAH Universitas Padjadjaran, Sumedang 45363
  • Eulis Tanti MARLINA Universitas Padjadjaran, Sumedang 45363
  • Ellin HARLIA Universitas Padjadjaran, Sumedang 45363

Abstract

Contamination of microorganism in poultry incubator is one of the causes of low egg hatchability. To increase hatchability, maintaining sanitation of poultry incubator is a must. Neem leaves have antibacterial compounds that can be used as a natural disinfectant in poultry incubator. The aim of this research is to determine the effect of neem leaves as a natural disinfectant towards the decreasing number of bacteria and fungi in poultry incubator. Completely randomized design is used as the experimental method with three treatments of neem leaves solution, namely P1 (50%), P2 (75%), and P3 (100%) and six replications. Variables observed of this study were the decreasing number of bacteria and fungi, inhibitory zone on bacterial growth, and identification of fungi in poultry incubator. The result showed that solution of neem leaves with various concentrations had similar effects on the decreasing number of bacteria and fungi in poultry incubator. Based on analysis of variance, all treatments had no significant effect on inhibitory test of coccus bacteria. The higher concentration of neem leaves, the larger the inhibitory zone formed. The treatment of various neem leaves decreased number of bacteria in poultry incubator by 56.16% (P1), 57.94% (P2), and 62.20% (P3), while fungi by 57.63% (P1), 58.11% (P2), and 57.65% (P3). The statistical analysis showed that all treatments had no significant effect on the decreasing number of bacteria and fungi in poultry incubator. Based on fungal identification, neem leaves affected five types of fungi namely; Aspergillus ochraceus, Penicillium sp., Cladosporium sp., Mucor sp., and Geothricum sp.

References

Apristiani, D. & Astuti, P. (2005). Isolasi komponen aktif antibakteri ekstrak kloroform. Daun mimba (Azadirachta indica A. Juss.) dengan bioautografi. Biofarmasi 3(2), 43-46.

Ayini, U., Harnina, S., & Dewi, T.C. (2014). Efek antibakteri ekstrak daun mimba terhadap bakteri vibrio algynoliticus secara in vitro. Biosaintifika. Journal of Biology and Biology Education 6(1), 67-75.

Biu, A.A., Yusuf, S.D., & Rabo, J.S. (2009). Phytochemical screening of Azadiractha indica (Neem) (Meliceae) in Maiduguri, Nigeria. Biosince Research Communications 21(6), 281-283.

Ducel, G., Febry, J., &Nicolle, L. (2002). Prevention of nosocomial infection. United States: World Health Organization.

Eckman, M.K., & Morgan-Jones, G. (1978). Fungus species isolated from commercial hatcheries in alabama. Avian Diseases 23(1), 204-208.

Fardiaz, S. (1992). Mikrobiologi pangan 1. Jakarta: Gramedia Pustaka Utama.

Mauseth, J.D. (2013). Botany: An introduction to plant biology. 5th eds. Burlington: Jones and Bartlett Learning.

Mycek, M.J., Harvey, R.A., Champe, P.C., & Fisher, B.D. (2001). Lippincottt’s illustrated reviews: Farmacology. Jakarta: Widya Medika.

Ngajow, M., Abidjulu, J., & Kamu, V.S. (2011). Pengaruh antibakteri ekstrak kulit batang matoa (Pometia pinnata) terhadap bakteri Staphylococcus aureus secara in vitro. Jurnal MIPA Unsrat Online 2(2), 128-132.

Pritima, R.A., & Pandian, R.S. (2008). Antibacterial potency of crude extracts of Azadirachta indica A. juss (leaf) against microbes causing reproductive tract infections among women. Current Biotica 2(2), 193-205.

Sarmiento, W.C., Maramba, C.C., & Gonzales, M.L.M. (2011). An in vitro study on the antibacterial effect of neem (Azadirachta indica) leaf extract on methicillin-sensitive and Methicillin-resistant Staphylococcus aureus. PIDSP Journal 12(1), 40-45.

Shrivastava, D.K., & Swarnkar, K. (2014). Anitifungal activity of leaf extract of neem (Azadirachta indica Linn.). Internationa Journal of Current Microbiology and Applied Sciences 3(5), 305-308.

Sugianitri, N.K. (2011). Ekstrak Biji Buah Pinang (Areca catechu L.) dapat Menghambat Pertumbuhan Koloni Candida albicans secara in vitro pada Resin Akrilik Heat Cured. Thesis, Universitas Udayana, Bali.

Tripathi, A.K., & Bhatnagar, M.K. (2012). Physico-chemical study of foliar chemicals of neem (Azadirachta indica A. juss) in different seasons, around Amarkantak thermal power plant, Chachai (Madhya Paradesh) India. International Journal of Science and Research 3(11), 119-123.

Wahdjuni, S., Puspawati, N.M., & Arista, N.P.R.E. (2016). Isolasi dan identifikasi senyawa aktif antijamur dari daun mimba (Azadirachta indica A. juss) sebagai pengendali jamur Fusarium sp. pada tanaman buah naga (Hylocereus sp.). Jurnal Kimia 10(2), 197-203.

Widiastuti, R., Darminto, S., Bahri, S., & Firmansyah, R. (2003). Inokulasi aflatoksin B1 pada telur berembrio dan residunya pada ayam menetas. Pros. Seminar nasional teknologi peternakan dan veteriner. Bogor 2003, 462-466.

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Published

2019-03-01

Issue

Vol. 2019 No. 1 (2019): The 4th Industrial Revolution and Its Impacts

Section

Abstract