TY - JOUR AU - PRASONGSOM, Sasikarn AU - THAMMASIRI, Kanchit AU - NARANGAJAVANA, Jarunya AU - THITAMADEE, Siripong AU - CHUENBOONNGARM, Ngarmnij AU - PANVISAVAS, Nathinee PY - 2019/04/04 Y2 - 2024/03/29 TI - Cryopreservation of Dendrobium cruentum Rchb. f. Seeds by D Cryo-plate and V Cryo-plate Techniques JF - Walailak Journal of Science and Technology (WJST) JA - Walailak J Sci & Tech VL - 17 IS - 3 SE - Research Article DO - 10.48048/wjst.2020.6159 UR - https://wjst.wu.ac.th/index.php/wjst/article/view/6159 SP - 181-191 AB - <p><em>Dendrobium cruentum</em> Rchb. f. is a native Thai orchid species that has faced extinction because of its attractive characteristics. Consequently, conservation of this species is urgently needed. In this study, cryopreservation technique was applied to <em>D. cruentum</em> Rchb. f. seeds for long-term conservation. A successful protocol for <em>D. cruentum</em> Rchb. f. seed cryopreservation was developed by using D cryo-plate and V cryo-plate techniques. Seed viability was tested by TCC solution and 93.8 % of dyed seeds were shown. For cryo-plate technique, seeds were encapsulated over the cryo-plate by using 2 % (w/v) sodium alginate and polymerized with 100 mM CaCl<sub>2</sub>. Encapsulated seeds were desiccated by using a laminar airflow and PVS2 solution treatment with the same exposure time (0, 30, 60, 90, and 120 min). After cryopreservation, encapsulated seeds were cultured on modified VW agar medium. From the results, the maximum germination and regrowth percentage were observed; D cryo-plate technique with 60 min of dehydration time gave the highest germination (68.9 %) and regrowth (57.8 %). Thus, the excess of dehydration may cause the reduction of germination and plant regeneration. In conclusion, D cryo-plate technique proved to be appropriate for <em>D. cruentum</em> Rchb. f. seed cryopreservation.</p> ER -