TY - JOUR AU - VONGSAK, Boonyadist AU - CHONANANT, Chirapond AU - MACHANA, Sasipawan PY - 2016/10/10 Y2 - 2024/03/29 TI - In Vitro Cytotoxicity of Thai Stingless Bee Propolis from Chanthaburi Orchard JF - Walailak Journal of Science and Technology (WJST) JA - Walailak J Sci & Tech VL - 14 IS - 9 SE - Research Article DO - UR - https://wjst.wu.ac.th/index.php/wjst/article/view/2265 SP - 741-747 AB - <em><em></em></em><p>Stingless bee propolis has a complex chemical composition and has been reported to possess various pharmacological properties. Chanthaburi province is the major source of stingless bee propolis production in Thailand. In the present study, the cytotoxic activities of the crude extracts of 3 different stingless bee (<em>Tetragonula pagdeni</em>, <em>Lepidotrigona ventralis</em>,<em> </em>and<em> Lepidotrigona terminata</em>) propolis from the same orchard were determined by sulphorhodamine assay against 4 human cancer cell lines (KB, HepG2, Caco-2, and SK-MEL-28) and a normal human cell line (Hs68). <em>T. pagdeni</em> propolis extract expressed the highest cytotoxicity on cancer cells, but a low toxicity on normal cells. On the contrary, <em>L. ventralis </em>and<em> L. terminata</em> propolis extracts demonstrated low cytotoxicity against cancer cell lines. Compounds from <em>T. pagdeni</em> propolis were investigated and elucidated as gamma and alpha mangostin. The cytotoxicity of these pure compounds was comparable to doxorubicin, a positive control. These compounds may be responsible for the cytotoxicity activity on cancer cells of <em>T. pagdeni</em> propolis from a Chanthaburi plantation.</p> ER -