TY - JOUR AU - MANGUNTUNGI, Baso AU - MUSTOPA, Apon Zaenal AU - MEILINA, Lita AU - NURFATWA, Maritsa AU - VANGGY, Leggina Rezzy AU - IRAWAN, Shasmita AU - TAMZIL, Mohamed Sahrul AU - APRILIAN, Tegar AU - Yulianti, Yulianti AU - FIDDUHA, Arsyadila Sophia AU - WERSIAN, Intan Nurani PY - 2021/07/31 Y2 - 2024/03/29 TI - The Profile Analysis of Lactic Acid Bacteria (LAB) from Sumbawa White Honey and Its Potential Producing Antibacterial Compounds JF - Walailak Journal of Science and Technology (WJST) JA - Walailak J Sci & Tech VL - 18 IS - 15 SE - Research Article DO - 10.48048/wjst.2021.22204 UR - https://wjst.wu.ac.th/index.php/wjst/article/view/22204 SP - Article 22204 (12 pages) AB - <p>Honey acts as an antibacterial without side effects, and also contains antiseptic substances which function to inhibit bacterial growth. This study aimed to isolate the Lactic Acid Bacteria (LAB) in Sumbawa white honey and the bioactive compounds produced as pathogenic antibacteria. The 1<sup>st</sup> stage in this study was the isolation of lactic acid bacteria (LAB) in Sumbawa white honey, then continued with a grading test, morphological test, catalase test, methyl red test, and the last test, namely the antimicrobial test against 5 pathogenic bacteria (<em>Salmonellatyhposa</em>,<em> Staphylococcus aureus</em>,<em> Escherichia coli</em>,<em> Enterobacter ludwigii</em>, and <em>Leclerciaadecarboxylata</em>). Data analysis was performed using the Analysis of variance (ANOVA) test at a confidence level of 0.05 with SPSS 24. Based on the results of sequencing analysis, it was found that the 5 selected isolates were <em>Enterococcus faecium </em>species. The <em>Enterococcus faecium</em> species obtained from the sequencing results had different strains. The accession numbers of the 5 <em>Enterococcus faecium</em> were: Isolate-03 with a percentage of 97.29 % (accession number: KU324920.1), Isolate-07 has a percent identity of 97.36 % (accession number: MF108201.1), Isolate-09 of 97.73 % (accession number: CP041261.3), Isolate-20 with a percentage of 96.40 % (accession number: MN511819.1), and Isolate-24 with a percentage of 98.61 % (accession number: KM495938.1). These isolates can inhibit the growth of all tested pathogenic bacteria treated with 100 % LAB metabolites and were not significantly different (<em>p</em> &gt; 0.05) compared to a positive control (Ampicillin).</p><p><strong>HIGHLIGHTS</strong></p><ul><li>Antibacterial compound of Lactic Acid Bacteria (LAB) from Sumbawa white honey</li><li>Lactic acid bacteria isolation, characterization, and biosprosprection against pathogens</li><li>Identified LAB by 16s rRNA sequencing gives five strains of <em>Enterococcus faecium</em></li><li>All identified LAB metabolites can inhibit all pathogens by similar inhibition percentage with Ampicillin</li></ul><p><strong>GRAPHICAL ABSTRACT</strong><br><img src="https://wjst.wu.ac.th/public/site/images/admin/fig-0-1.jpg" alt="" width="1349" height="407"></p> ER -