@article{IMSOMBOON_THAMMASIRI_KOSIYACHINDA_CHUENBOONNGARM_PANVISAVAS_2018, title={Cryopreservation of Protocorm-like Bodies of Vanda lilacina Teijsm. & Binn., a Thai Orchid Species, by V-cryo-plate and D-cryo-plate Methods}, volume={17}, url={https://wjst.wu.ac.th/index.php/wjst/article/view/4429}, DOI={10.48048/wjst.2020.4429}, abstractNote={<p>Protocorm-like bodies (PLBs) of<em> Vanda lilacina </em>Teijsm. & Binn. were used to study two new cryopreservation methods, the V-cryo-plate and D-cryo-plate methods, for survival score and percentage of survival. PLBs were dehydrated with PVS2 solution (0, 20, 40, 60, and 120 min) and silica gel (0, 0.5, 1, 1.5, 2, 2.5, and 3 h) for the V-cryo-plate and D-cryo-plate methods, respectively. The results showed that the V-cryo-plate method, dehydration with PVS2 solution for 20 min, was a suitable method. It gave a survival score of 0.31, higher than control (+LN; 0 min) and gave a percentage of survival of 33.33 %. For the D-cryo-plate method, the suitable method for cryopreserved PLBs of <em>V. lilacina</em> was dehydration with silica gel for 1 h. It gave the highest survival score of 0.78 and percentage of survival was up to 83.78 %. Then, an unpaired t-test was used to compare data of survival scores of cryopreserved <em>V. lilacina</em> PLBs between the V-cryo-plate and D-cryo-plate methods. The best suitable cryopreservation method of <em>V. lilacina</em> PLBs is the D-cryo-plate method using silica gel for 1 h in dehydration step.</p>}, number={4}, journal={Walailak Journal of Science and Technology (WJST)}, author={IMSOMBOON, Tagrid and THAMMASIRI, Kanchit and KOSIYACHINDA, Pahol and CHUENBOONNGARM, Ngarmnij and PANVISAVAS, Nathinee}, year={2018}, month={May}, pages={369–379} }