@article{LERTWORAPREECHA_TUYARUM_TANOMTONG_SUWANNAPOOM_KIRIRATNIKOM_2021, title={Establishment of Cell Lines from Slender Walking Catfish Clarias nieuhofii (Valenciennes, 1840)}, volume={18}, url={https://wjst.wu.ac.th/index.php/wjst/article/view/10857}, DOI={10.48048/wjst.2021.10857}, abstractNote={<p>The slender walking catfish (<em>Clarias nieuhofii</em> Valenciennes, 1840) is a freshwater fish found in the ecosystems within the peat swamp forests of South-East Asian countries, including Thailand. In this study, a new testis fibroblast-like (FLT) and ovary epithelial-like (ELO) cells derived from <em>C</em><em>. nieuhofii</em> (Valenciennes, 1840) was established. The optimal number of both cells was determined, and the optimal starting concentration of both cells is approximately 10<sup>5</sup> cellsžml<sup>-1</sup>. The FLT and ELO can grow best at 28 °C in DMEM supplemented with 20, 15, and 10 % fetal bovine serum (FBS). Chromosome analysis revealed that the typical diploid chromosome number was 66 (2n) in <em>C</em><em>. nieuhofii</em> (Valenciennes, 1840). In both males and females, the elementary number of chromosomes (NF; the number of chromosome arms) was 98. No heteromorphic chromosome was observed. Four types of chromosomes were found in this species, including 14 metacentric, 20 submetacentric, 2 acrocentric, and 30 telocentric chromosomes. The FLT and ELO cells were maintained up to 6 months with more than 20 passages successfully cryopreserved and thawed.</p> <p><strong>HIGHLIGHTS</strong></p> <ul> <li>Two new fibroblast-like cell from testis and epithelium-like cell from ovary, derived from slender walking catfish<em> Clarias nieuhofii </em>(Valenciennes, 1840) have been successfully established by explant outgrowth technique</li> <li>Indicated the optimal cell density, fetal bovine serum and types of culture media for growing of both cells</li> <li>Chromosome analysis revealed that the typical diploid chromosome number is 66 (2n) in <em>Clarias nieuhofii </em>(Valenciennes, 1840)</li> </ul> <p><strong>GRAPHICAL ABSTRACT</strong><br /><img src="https://wjst.wu.ac.th/public/site/images/admin/graphical-abstract.jpg" alt="" width="960" height="540" /></p>}, number={12}, journal={Walailak Journal of Science and Technology (WJST)}, author={LERTWORAPREECHA, Monthon and TUYARUM, Chiraprapha and TANOMTONG, Alongklod and SUWANNAPOOM, Chatmongkon and KIRIRATNIKOM, Suphada}, year={2021}, month={Jun.}, pages={Article 10857 (10 pages)} }