Hypoxic and Normoxic-Human Wharton’s Jelly Mesenchymal Stem Cell-Free Lysate for Anticancer Therapies

Wahyu  WIDOWATI; Diana Krisanti  JASAPUTRA; Hanna Sari Widya  KUSUMA; Rizal  RIZAL; Dian Ratih  LAKSMITAWATI; Dwi Surya  ARTIE; Ika Adhani  SHOLIHAH; Dewani Tediana  YUSEPANY; Riyani  LESTARI; Rr. Anisa Siwianti  HANDAYANI; Seila  ARUMWARDANA; Harry  MURTI; Indra  BACHTIAR; Mawar  SUBANGKIT

doi:10.48048/wjst.2021.9270

Authors

Wahyu WIDOWATI Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia
Diana Krisanti JASAPUTRA Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia
Hanna Sari Widya KUSUMA Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
Rizal RIZAL Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia
Dian Ratih LAKSMITAWATI Faculty of Pharmacy, University of Pancasila, Jakarta 12640, Indonesia
Dwi Surya ARTIE Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
Ika Adhani SHOLIHAH Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
Dewani Tediana YUSEPANY Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
Riyani LESTARI Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
Rr. Anisa Siwianti HANDAYANI Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
Seila ARUMWARDANA Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia
Harry MURTI Stem Cell and Cancer Institute, Jakarta 13210, Indonesia
Indra BACHTIAR Stem Cell and Cancer Institute, Jakarta 13210, Indonesia
Mawar SUBANGKIT Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, IPB University, Bogor 16680, West Java, Indonesia

DOI:

https://doi.org/10.48048/wjst.2021.9270

Keywords:

Anticancer, Cell-free lysate, Hypoxia, Mesenchymal stem cells, Wharton’s jelly

Abstract

Cancer can be defined as a disease in which a group of uncontrolled cells growth by ignoring the rules of normal cell division. Mesenchymal Stem Cells (MSCs) are one of the promising cell-based therapies for anticancer therapies. Preceding research had disclosed that conditioned medium from hypoxic-treated human Wharton’s jelly mesenchymal stem cells (hWJMSCs-hypoCM) and normoxic-treated hWJMSCs (hWJMSCs-norCM) can inhibit the proliferation of various cancer cell lines and have no cytotoxic effect on normal cells. The present study, hence, measured the effect of a human Wharton’s jelly stem cells cell-free lysate (hWJMSCs-CL), cultured with hypoxic condition (hWJMSCs-hypoCL), and normoxic condition (hWJMSCs-norCL) towards several cancer cell lines including ovarian (SKOV3), cervical (HeLa), tongue squamosa (HSC3), and liver (HepG2) proliferation. Human mesenchymal stem cells (hMSCs), human fibroblast cells, and mouse fibroblast cells (NIH3T3) were used as control. Both hWJMSCs-CL grown in hypoxic and normoxic conditions could inhibit the proliferation of various cancer cell lines with median inhibitory concentration (IC₅₀) 21.09 - 95.92 μg/mL and could cause low inhibition of the normal cells with IC₅₀ 409.19 - 799.74 μg/mL. The hWJMSCs-hypoCL and hWJMSC-norCL could inhibit various cancer and were not harmful to normal cells.

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