Hypoxic and Normoxic-Human Wharton’s Jelly Mesenchymal Stem Cell-Free Lysate for Anticancer Therapies
Keywords:Anticancer, Cell-free lysate, Hypoxia, Mesenchymal stem cells, Wharton’s jelly
Cancer can be defined as a disease in which a group of uncontrolled cells growth by ignoring the rules of normal cell division. Mesenchymal Stem Cells (MSCs) are one of the promising cell-based therapies for anticancer therapies. Preceding research had disclosed that conditioned medium from hypoxic-treated human Wharton’s jelly mesenchymal stem cells (hWJMSCs-hypoCM) and normoxic-treated hWJMSCs (hWJMSCs-norCM) can inhibit the proliferation of various cancer cell lines and have no cytotoxic effect on normal cells. The present study, hence, measured the effect of a human Wharton’s jelly stem cells cell-free lysate (hWJMSCs-CL), cultured with hypoxic condition (hWJMSCs-hypoCL), and normoxic condition (hWJMSCs-norCL) towards several cancer cell lines including ovarian (SKOV3), cervical (HeLa), tongue squamosa (HSC3), and liver (HepG2) proliferation. Human mesenchymal stem cells (hMSCs), human fibroblast cells, and mouse fibroblast cells (NIH3T3) were used as control. Both hWJMSCs-CL grown in hypoxic and normoxic conditions could inhibit the proliferation of various cancer cell lines with median inhibitory concentration (IC50) 21.09 - 95.92 μg/mL and could cause low inhibition of the normal cells with IC50 409.19 - 799.74 μg/mL. The hWJMSCs-hypoCL and hWJMSC-norCL could inhibit various cancer and were not harmful to normal cells.
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