The Effect of the Aqueous Enzymatic Extraction Method towards Momordica charantia Seed Oil and Its Lignocellulosic Biomass

Nadirah Zawani  MOHD NESFU; Hasnah  OSMAN; Suriyati  MOHAMAD; Lionel  MUNIGLIA; Nicolas  BROSSE; Dominique  LAURAIN-MATTAR; Rosella  SPINA; Ezatul E.  KAMARULZAMAN

doi:10.48048/wjst.2021.9098

Authors

Nadirah Zawani MOHD NESFU School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
Hasnah OSMAN School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
Suriyati MOHAMAD School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
Lionel MUNIGLIA LiBio, ENSAIA, 2 Avenue de la Forêt de Haye, Université de Lorraine, 54505 Vandoeuvre-lès-Nancy, France
Nicolas BROSSE LERMAB, Faculté des Sciences et Technologies BP 70239, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France
Dominique LAURAIN-MATTAR UMR CNRS 7565 SRSMC, Faculté des Sciences et Technologies BP 70239, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France
Rosella SPINA UMR CNRS 7565 SRSMC, Faculté des Sciences et Technologies BP 70239, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France
Ezatul E. KAMARULZAMAN School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia

DOI:

https://doi.org/10.48048/wjst.2021.9098

Keywords:

Momordica charantia L., Seeds, Aqueous enzymatic extraction, Lignocellulosic biomass

Abstract

The development of a sustainable product using natural resources will gain much attention nowadays. This work aimed to study the effect of aqueous enzymatic extraction (AEE) using a different ratio of enzyme cocktails HEL1 and X7 towards the production of Momordica charantia L. (M. charantia) seed oil and to identify the composition of the seeds’ lignocellulosic biomass (extracted-free materials, EFM). The M. charantia seed oil contained omega-3 fatty acids, such as stearidonic acid, that possess hypotriglyceridemic properties. The oil obtained was derivatized to fatty acid methyl esters (FAMEs) before biochemical quantification using gas chromatography (GC). The EFM was subjected to Soxhlet extraction and further analyzed to identify lignin, acid-soluble sugars, and hemicellulose composition. The results showed that the highest seed oil percentage, (6.26±0.53 %) was extracted from M2 sample using a combination of 5 % HEL1 to 1.25 % X7 enzyme cocktails, as compared to the extraction without enzyme (3.32±0.38 %). The percentage of stearidonic acid in the seed oil increased from 13.55 % (without enzyme) to 19.43 % (M2), proving that there were some change in terms of fatty acid composition in the seed oil.

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References

TK Behera, S Behera, LK Bharathi, KJ John, PW Simon and JE Staub. Horticulture reviews. In: J Janick (Ed.). Bitter Gourd: Botany, Horticulture, Breeding. Wiley Blackwell, 2010, p. 101-41.

A Donya, N Hettiarachchy, R Liyanage, JJ Lay, P Chen and M Jalaluddin. Effects of processing methods on the proximate composition and momordicosides K and L content of bitter melon vegetable. J. Agric. Food Chem. 2007; 55, 5827-33.

PR Dandawate, D Subramaniam, SB Padhye and S Anant. Bitter melon: A panacea for inflammation and cancer. Chin. J. Nat. Med. 2016; 14, 81-100.

K Abascal and E Yarnell. Using bitter melon to treat diabetes. J. Altern. Compl. Ther. 2005; 11, 179-84.

TE Shain, A Abdullah and K Kassim. Extraction of steroidal glycoside from small-typed bitter gourd (Momordica charantia L.). J. Chem. Pharm. Res. 2015; 7, 870-8.

B Joseph and D Jini. Antidiabetic effects of Momordica charantia (bitter melon) and its medicinal potency. Asian Pac. J. Trop. Dis. 2013; 3, 93-102.

S Desai and P Tatke. Charantin: An important lead compound from Momordica charantia for the treatment of diabetes. J. Pharmacogn. Phytochem. 2015; 3, 163-6.

TB Ng, WW Li and HW Yeung. Effects of ginsenosides, lectins and Momordica charantia insulin-like peptide on corticosterone production by isolated rate adrenal cells. J. Ethnopharmacol. 1987; 21, 21-9.

GT Zhao, JQ Liu, YY Deng, HZ Li, JC Chen, ZR Zhang, L Zhou and MH Qiu. Cucurbitane-type triterpenoids from the stems and leaves of Momordica charantia. Fitoterapia 2014; 95, 75-82.

JQ Cao, Y Zhang, JM Cui and YQ Zhao. Two new cucurbitane triterpenods from Momordica charantia L. Chin. Chem. Lett. 2011; 22, 583-6.

Y Tasui, M Hosokawa, T Sahara, R Suzuki, S Ohgiya, H Kohno, T Tanaka and K Miyashita. Bitter gourd seeds fatty acid rich in 9c,11t,13t-conjugated linolenic acid induces apoptosis and up-regulates the GADD45, p53 and PPARγ in human colon cancer Caco-2 cells. Prostaglandins Leukot. Essent. Fatty Acids 2005; 73, 113-9.

L Liu, EG Hammond and BJ Nikolau. In Vivo studies of the biosynthesis of a-eleostearic acid in the seed of Momordica charantia L. Plant Physiol. 1997; 113, 1343-9.

A Elagizi, CJ Lavie, K Marshall, JJ DiNicolantonio, JH O’Keefe and RV Milani. Omega-3- polyunsaturated fatty acids and cardiovascular health: A comprehensive review. Prog. Cardiovasc. Dis. 2018; 61, 76-85.

E Balk, M Chung, A Lichtenstein, P Chew, B Kupelnick, A Lawrence, DD Vine and J Lau. Effects of omega-3 fatty acids on cardiovascular risk factors and intermediate markers of cardiovascular disease. Evid. Rep. Technol. Assess. 2004; 93, 1-6.

GC Chen, HM Su, YS Lin, PY Tsou, JH Chyuan and PM Chao. A conjugated fatty acid present at high levels in bitter melon seed favorably affects lipid metabolism in hepatocytes by increasing NAD+/NADH ratio and activating PPARα, AMPK and SIRT1 signalling pathway. J. Nutr. Biochem. 2016; 33, 28-35.

Department of Agriculture, Putrajaya, Malaysia. Vegetables and cash crops statistic. Department of Agriculture, Putrajaya, Malaysia, 2014.

G Sodeifian, NS Ardestani, SA Sajadian and K Moghadamian. Properties of Portulaca oleracea seed oil via supercritical fluid extraction: Experimental and optimization. J. Supercrit. Fluids 2018; 135, 34-44.

MM Yusoff, MH Gordon, O Ezeh and K Niranjan. Aqueous enzymatic extraction of Moringa oleifera oil. Food Chem. 2016; 211, 400-8.

RC Kumar, MM Benal, BD Prasad, MS Krupashankara, RS Kulkarni and NH Siddaligaswamy, Microwave assisted extraction of oil from Pongamia pinnata seeds. Mater. Today Proc. 2018; 5, 2960-4.

RA Sheldon and SV Pelt. Enzyme immobilisation in biocatalysis: Why, what and how. Chem. Soc. Rev. 2013; 42, 6223-35.

KW Waldron, ML Parker and AC Smith. Plant cell walls and food quality. Compr. Rev. Food Sci. Food Saf. 2003; 2, 101-19.

NC Carpita and DM Gibeaut. Structural models of primary cell walls in flowering plants: Consistency of molecular structure with the physical properties of the walls during growth. Plant J. 1993; 3, 1-30.

A Rosenthal, DL Pyle and K Niranjan. Aqueous and enzymatic processes for edible oil extraction. Enzyme Microb. Technol. 1996; 19, 402-20.

S Latif and F Anwar. Aqueous enzymatic sesame oil and protein extraction. Food Chem. 2011; 125, 679-84.

L Muniglia, M Girardin, B Piffaut and G Ricochon. WO2011/045387 A1. Institut National De La Propriété Industrielle. 2011, Available at http://patentscope.wipo.int/search/en/WO2011045387, accessed March 2020.

LD Metcalfe and AA Schmitz. The rapid preparation of fatty acid esters for gas chromatographic analysis. Anal. Chem. 1961; 33, 363-4.

M Chadni, N Grimi, O Bals, I Ziegler-Devin and N Brosse. Steam explosion process for the selective extraction of hemicelluloses polymers from spruce sawdust. Ind. Crop. Prod. 2019; 141, 111757.

KC Baby and TV Ranganathan. Effect of enzyme pre-treatment on extraction yield and quality of cardamom (Elettaria cardamomum maton) volatile oil. Ind. Crop. Prod. 2016; 89, 200-6.

QY Gai, J Jiao, PS Mu, W Wang, M Luo, CY Li, YG Zu, FY Wei and YJ Fu. Microwave-assisted aqueous enzymatic extraction of oil from Isatis indigotica seeds and its evaluation of physicochemical properties, fatty acid compositions and antioxidant activities. Ind. Crop. Prod. 2013; 45, 303-11.

GPP Kamatou and AM Viljoen. Comparison of fatty acid methyl esters of palm and palmist oils determined by GCxGC-ToF-MS and GC-MS/FID. S. Afr. J. Bot. 2017; 112, 483-8.

SN Baharum, H Bunawan, MA Ghani, WAW Mustapha and NM Noor. Analysis of the chemical composition of the essential oil of Polygonum minus Huds. Using two-dimensional gas chromatography-time-of-flight mass spectrometry (GC-TOF MS). Molecules 2010; 15, 7006-15.

AR White and RM Brown. Enzymatic hydrolysis of cellulose: Visual characterization of the process. Proc. Natl. Acad. Sci. USA 1981; 78, 1047-51.

C Wu, Y Xiao, W Lin, J Li, S Zhang, J Zhu and J Rong. Aqueous enzymatic process for cell wall degradation and lipid extraction from Nannochloropsis sp. Bioresour. Technol. 2017; 223, 312-6.

A Zuorro, V Malavasi, G Cao and R Lavecchia. Use of cell wall degrading enzymes to improve the recovery of lipids from Chlorella sorokiniana. Chem. Eng. J. 2019; 377, 120325.

JJ Liu, MAA Gasmalla, P Li and R Yang. Enzyme-assisted extraction processing from oilseeds: Principle, processing and application. Innov. Food Sci. Emerg. Technol. 2016; 35, 84-193.

SM Abdulkarim, K Long, OM Lai, SKS Muhammad and HM Ghazali. Some physico-chemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food Chem. 2005; 93, 253-63.

MA Ali, MA Sayeed, MS Reza, MS Yeasmin and AM Khan. Characteristics of seed oils and nutritional compositions of seeds from different varieties of Momordica charantia Linn. cultivated in Bangladesh. Czech J. Food Sci. 2008; 26, 275-83.

CM Welker, VK Balasubramanian, C Petti, KM Rai, S DeBolt and V Mendu. Review: Engineering plant biomass lignin content and composition for biofuels and bioproducts. Energies 2015; 8, 7654-76.

A Biz, FC Farias, FA Motter, DH de Paula, P Richard, N Krieger and DA Mitchell. Pectinase activity determination: An early deceleration in the release of reducing sugars throws a spanner in the works. PLoS One 2014; 9, 1-8.

DB Pedrolli, AC Monteiro, E Gomes and EC Carmona. Pectin and Pectinases: Production, characterization and industrial application of microbial pectinolytic enzymes. Open Biotechnol. J. 2009; 3, 9-18.

L Liu, Y You, H Deng, Y Guo and Y Meng. Promoting hydrolysis of apple pomace by pectinase and cellulase to produce microbial oils using engineered Yarrowia lipolytica. Biomass Bioenerg. 2019; 126, 62-9.

F Segato, ARL Damásio, RC de Lucas, FM Squina and RA Prade. Genomics review of holocellulose deconstruction by aspergilli. Microbiol. Mol. Biol. Rev. 2014; 78, 588-613.

L Zhong, JF Matthews, MF Crowley, T Rignall, C Talón, JM Cleary, RC Walker, G Chukkapalli, CM Cabe, MR Nimlos, CL Brooks, ME Himmel and JW Brady. Interactions of the complete cellobiohydrolase I from Trichoderma reesei with microcrystalline cellulose Iβ, Cellulose 2008; 15, 261-73.

Y Wei, D Wu, D Wei, Y Zhao, J Wu, X Xie, R Zhang and Z Wei. Improved lignocellulose-degrading performance during straw composting from diverse sources with actinomycetes inoculation by regulating the key enzyme activities. Bioresour. Technol. 2019; 271, 66-74.

J Nill, N Karuna and T Jeoh. The impact of kinetic parameters on cellulose hydrolysis rates. Process Biochem. 2018; 74, 108-17.

B Yang, Z Dai, SY Ding and CE Wyman. Enzymatic hydrolysis of cellulosic biomass. Biofuels 2011; 2, 421-50.

J Zhao, Z Dong, J Li, L Chen, Y Bai, Y Jia and T Shao. Ensiling as pretreatment of rice straw: The effect of hemicellulase and Lactobacillus plantarum on hemicellulose degradation and cellulose conversion. Bioresour. Technol. 2018; 266, 158-65.

PF Ávila, MBS Forte and R Goldbeck. Evaluation of the chemical composition of a mixture of sugarcane bagasse and straw after different pre-treatments and their effects on commercial enzyme combinations for the production of fermentable sugars. Biomass. Bioenerg. 2018; 116, 180-8.

CM Popescu, MC Popescu and C Vasile. Characterization of fungal degraded lime wood by FT-IR and 2D IR correlation spectroscopy. Microchem. J. 2010; 95, 377-87.

X Peng, J Bian, M Li, X Xiao, X Xia, W Yin and R Sun. Graded ethanol fractionation and structural characterization of alkali-extractable hemicelluloses from Olea europaea L. Bioresources 2013; 8, 1110-23.

DS Naidu, SP Hlangothi and MJ John. Bio-based products from xylan: A review. Carbohydr. Polym. 2018; 179, 28-41.

M Chadni, O Bals, I Ziegler-Devin, N Brosse and N Grimi. Microwave-assisted extraction of high-molecular-weight hemicelluloses from spruce wood. Comptes Rendus Chimie 2019; 22, 574-84.

R Sun, JM Lawther and WB Banks. Fractional and structural characterization of wheat straw hemicelluloses. Carbohydr. Polym. 1996; 29, 325-31.

T Eremeeva. Size-exclusion chromatography of enzymatically treated cellulose and related polysaccharides: A review. J. Biochem. Bioph. Meth. 2003; 56, 253-64.

MS Izydorczyk and CG Biliaderis. Cereal arabinoxylans: Advances in structure and physicochemical properties. Carbohydr. Polym. 1995; 28, 33-48.