The Effect of the Aqueous Enzymatic Extraction Method towards Momordica charantia Seed Oil and Its Lignocellulosic Biomass


  • Nadirah Zawani MOHD NESFU School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
  • Hasnah OSMAN School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
  • Suriyati MOHAMAD School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
  • Lionel MUNIGLIA LiBio, ENSAIA, 2 Avenue de la Forêt de Haye, Université de Lorraine, 54505 Vandoeuvre-lès-Nancy, France
  • Nicolas BROSSE LERMAB, Faculté des Sciences et Technologies BP 70239, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France
  • Dominique LAURAIN-MATTAR UMR CNRS 7565 SRSMC, Faculté des Sciences et Technologies BP 70239, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France
  • Rosella SPINA UMR CNRS 7565 SRSMC, Faculté des Sciences et Technologies BP 70239, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France
  • Ezatul E. KAMARULZAMAN School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia



Momordica charantia L., Seeds, Aqueous enzymatic extraction, Lignocellulosic biomass


The development of a sustainable product using natural resources will gain much attention nowadays. This work aimed to study the effect of aqueous enzymatic extraction (AEE) using a different ratio of enzyme cocktails HEL1 and X7 towards the production of Momordica charantia L. (M. charantia) seed oil and to identify the composition of the seeds’ lignocellulosic biomass (extracted-free materials, EFM). The M. charantia seed oil contained omega-3 fatty acids, such as stearidonic acid, that possess hypotriglyceridemic properties. The oil obtained was derivatized to fatty acid methyl esters (FAMEs) before biochemical quantification using gas chromatography (GC). The EFM was subjected to Soxhlet extraction and further analyzed to identify lignin, acid-soluble sugars, and hemicellulose composition. The results showed that the highest seed oil percentage, (6.26±0.53 %) was extracted from M2 sample using a combination of 5 % HEL1 to 1.25 % X7 enzyme cocktails, as compared to the extraction without enzyme (3.32±0.38 %). The percentage of stearidonic acid in the seed oil increased from 13.55 % (without enzyme) to 19.43 % (M2), proving that there were some change in terms of fatty acid composition in the seed oil.


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How to Cite

MOHD NESFU, N. Z. ., OSMAN, H. ., MOHAMAD, S. ., MUNIGLIA, L. ., BROSSE, N. ., LAURAIN-MATTAR, D. ., SPINA, R. ., & KAMARULZAMAN, E. E. . (2021). The Effect of the Aqueous Enzymatic Extraction Method towards Momordica charantia Seed Oil and Its Lignocellulosic Biomass. Walailak Journal of Science and Technology (WJST), 18(6), Article 9098 (13 pages).