Bioactivity Determination and Development of Oil in Water Emulsion Containing Cassia fistula Bark Extract
Keywords:Cassia fistula, Tyrosinase inhibition, Free radical scavenging, Phytochemical analysis, Formulation
The determination of active contents, free radical scavenging, and tyrosinase inhibition activity of Cassia fistula extracts for skincare development is presented in this study. C. fistula was extracted by 95 % ethanol. The extracts were then determined for total phenolic content, phytochemical constituents, free radical scavenging, and tyrosinase inhibition activity. The formulation containing the selected C. fistula extract was prepared and examined for this formulation's bioactivities and stability. C. fistula extract from the bark provided the highest total phenolic content and free radical scavenging activity of 22.35±1.38 mgGAE/g and the IC50 value of 7.90±0.10 μg/mL, respectively. C. fistula extract from the bark was observed having tyrosinase inhibition activity at the IC50 value of 1.71×103±0.01 μg/mL and selected to be an active ingredient in oil in water emulsion. At 4±2 °C storage, the formulation containing C. fistula extract from bark showed the highest stability without changing the color, odor, homogeneity, while pH and viscosity were slightly changed. The formulation’s bioactivities containing C. fistula extract from bark were slightly decreased in all temperatures at the end of the stability test. In this study, C. fistula extract from the bark can potentially inhibit tyrosinase in a free radical scavenging activity. Therefore, it may be an appropriate choice for skincare products that aim to provide whitening effects.
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