Bioactive Compounds in Three Edible Lentinus Mushrooms
Keywords:
Anticancer, mushroom, Lentinus mushroom, antioxidant, bioactive proteinsAbstract
The generation of free radicals is a major issue in the development of carcinogenesis-induced mutations and tumor promotion. Bioactive compounds which are able to scavenge free radicals and provide protections against cancers caused by them are called antioxidants. We aimed to show that bioactive compounds from mushrooms will be valuable for health promotion. Catechin is a major group of phenolic compounds found in 3 species of mushroom, Lentinus squarrosulus, Lentinus polychrous and Lentinus edodes, especially in L. squarrosulus by LC-MS. The percentage of inhibition of free radical scavenging activity by DPPH assay was higher than 70 % in ethanolic extract of L. squarrosulus and L. polychrous, while in L. edodes it was lower as 30 %. Using FRAP to investigate the micromolar trolox equivalent of L. squarrosulus also showed it to be higher than the other 2 species. The highest amount of total phenolic compound was displayed in L. squarrosulus extract, which corresponds with the DPPH and FRAP assays. An anticancer protein, polysaccharide peptide (PSP), was also detected at a level of approximately 70 kDa via western immunoblotting. The amounts of PSP from protein extracts of L. squarrosulus, L. polychrous and L. edodes were 9.87 ± 0.03, 0.41 ± 0.03 and 0.17 ± 0.04 mg/g protein, respectively, by using ELISA method. The cell viability of human colorectal adenocarcinoma, HT-29, diminished significantly in the treatment group with protein extract from L. squarrosulus. This L. squarrosulus extract may be an effective natural product for clinical trials in patients, because these edible mushrooms are nontoxic and are also a consumable food for human health.doi:10.14456/WJST.2015.80
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F Yun-Zhong, Y Sheng and W Guoyao. Free radicals, antioxidants, and nutrition. Nutrition 2002; 18, 872-9.
A Jemal, F Bray, MM Center, J Ferlay, E Ward and D Forman. Global cancer statistics. CA: Cancer J. Clin. 2011; 61, 69-90.
T Jyothi. Cancer treatment using peptide: current therapies and future prospects. J. Amino Acid. 2012; 2012, 967347.
F Swaran. Structural, chemical and biological aspects of antioxidants for strategies against metal and metalloid exposure. Oxid. Med. Cell Longev. 2009; 2, 191-206.
L Ulrike, HJ Timo, J Niedermeyer and J Wolf-Dieter. The pharmacological potential of mushrooms. Evid. Based Complement. Alternat. Med. 2005; 2, 285-99.
TB Ng. Peptides and proteins from fungi. Peptide 2004; 25, 1055-73.
X Xiaofei, Y Huidan, C Jian and Z Xuewu. Bioactive proteins from mushrooms. Biotechnol. Adv. 2011; 29, 667-74.
C Jian and C Yusuf. Polysaccharopeptides of Coriolus versicolor: Physiological activity, uses, and Production. Biotechnol. Adv. 2003; 21, 109-22.
K Parris. The use of mushroom glucans and proteoglycans in cancer treatment. Altern. Med. Rev. 2000; 5, 4-27.
MY Kim, P Seguin, JK Ahn, JJ Kim, SC Chun, EH Kim, SH Seo, EY Kang, SL Kim, YJ Park, HM Ro and LM Chung. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J. Agric. Food Chem. 2008; 56, 7265-70.
MM Bradford. A dye binding assay for protein. Anal. Biochem. 1976; 72, 248-54.
UK Laemmli. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227, 680-5.
A Jongruk, P Pharima and U Prapaporn. Characterization of vitellin from the ovaries of the banana shrimp Litopenaeus merguiensis. Comp. Biochem. Physiol. 2006; 143, 27-36.
VL Singleton, R Orthofer and RM Lamuela-Raventos. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth. Enzymol. 1999; 299, 152-78.
MS Bloise. Antioxidant determination by the use of stable free radicals. Nature 1958; 181, 1199-2000.
IFF Benzie and JJ Strain. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal. Biochem. 1996; 239, 70-6.
M Sekete, D Ma, B Wang, H Wang and TB Ng. First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha. SpringerPlus 2012; 1, 79.
QH Liu, HX Wang and TB Ng. First report of a xylosespecific lectin with potent hemagglutinating, antiproliferative and anti-mitogenic activities from a wild ascomycete mushroom. Biochem. Biophys. Acta 2006; 1760, 1914-9.
M Li, G Zhang, H Wang and TB Ng. Purification and characterization of a laccase from the edible wild mushroom Tricholoma mongolicum. J. Microbiol. Biotechnol. 2010; 20, 1069-76.
L Tang, SC Shieh, GJ Lai, JY Wang, PH Ying, TH Ko and J Liang. Effect of a fungal immunomodulatory protein from Ganoderma tsugae on cell cycle and interferon-gamma production through phosphatidylinositol 3-kinase signal pathway. Process Biochem. 2008; 43, 423-30.
Z Xuanwei, J Hua, L Juan and T Kexuan. Cytotoxic activities of Coriolus versicolor (Yunzhi) extracts on human liver cancer and breast cancer cell line. Afr. J. Biotechnol. 2007; 6, 1740-3.
JM Wan, WH Sit, X Yang, P Jiang and LL Wong. Polysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cells. Chinese Med. 2010; 5, 16.
SU Luk, TK Lee, J Liu, DT Lee, YT Chiu and S Ma. Chemopreventive effect of PSP through targeting of prostate cancer stem cell-like population. PLoS One. 2011; 6, 19804.
CY Ho, CF Kim, KN Leung, KP Fung, TF Tse, H Chan and CBS Lau. Differential anti-tumor activity of Coriolus versicolor (Yunzhi) extract through p53- and/or Bcl-2-dependent apoptotic pathway in human breast cancer cells. Cancer Biol. Ther. 2005; 4, 638-44.
JH Kim, SJ Kim, HR Park, JI Choi, YC Ju, KC Nam, SJ Kim and SC Lee. The different antioxidant and anticancer activities depending on the color of oyster mushrooms. J. Med. Plants Res. 2009; 3, 1016-20.
TI Jeon, CH Jung, JY Cho, DK Park and JH Moon. Identification of an anticancer compound against HT-29 cells from Phellinus linteus grown on germinated brown rice. Asian Pac. J. Trop. Biomed. 2013; 3, 785-9.
D Vergote, C Cren-Olive, V Chopin, RA Toillon, C Rolando, H Hondermarck and X Le Bourhis. (-)-Epigallocatechin (EGC) of green tea induces apoptosis of human breast cancer cells but not of their normal counterparts. Breast Cancer Res. Treat. 2002; 76, 195-201.
BA Sutherland, MA Rahman and I Appleton. Mechanisms of action of green tea catechins, with a focus on ischemia-induced neurodegeneration. J. Nutr. Biochem. 2006; 17, 291-306.
NT Zaveri. Green tea and its polyphenolic catechins: Medicinal uses in cancer and noncancer applications. Life Sci. 2006; 78, 2073-80.
KT Chung, TY Wong, CI Wei, YW Huang and Y Lin. Tannins and human health: A review. Crit. Rev. Food Sci. Nutr. 1998; 38, 421-64.
P Chirinang and K Intarapichet. Amino acids and antioxidant properties of the oyster mushroom, Pleurotus ostreatus and Plerotus sajor-caju. ScienceAsia 2009; 35, 326-31.
HY Fu, DE Shieh and CT Ho. Antioxidant and free radical scavenging activities of edible mushrooms. J. Food Lipids. 2002; 9, 35-43.
IK Lee, YS Kim, YW Jang, JY Jang and BA Yun. New antioxidant polyphenols from the medicinal mushroom Innotus obliquus. Bioorg. Med. Chem. Lett. 2007; 17, 6678-81.
G Clarke, KN Ting, C Wiart and J Fry. High correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest. Antioxidants 2013; 2, 1-10.
NG Puttaraju, SU Venkateshaiah, SM Dharmesh, SMN Urs and R Somasundaram. Antioxidant activity of indigenous edible mushrooms. J. Agric. Food Chem. 2006; 54, 9764-72.
JH Yang, HC Lin, SM Dharmesh and JL Mau. Antioxidant properties of several commercial mushrooms. Food Chem. 2002; 77, 229-35.
P Zucca, A Rosa, CIG Tuberoso, A Piras, AC Rinaldi, E Sanjust, MA Dessi and A Rescigno. Evaluation of antioxidant potential of “Maltese Mushroom” (Cynomorium coccineum) by means of multiple chemical and biological assays. Nutrients 2013; 5, 149-61.
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