Reductive Dechlorination of 1,2-dichloroethane to Ethylene  by Anaerobic Enrichment Culture Containing Vulcanibacillus spp.

Utumporn  NGIVPROM; Nipa  MILINTAWISAMAI; Alissara  REUNGSANG

doi:10.48048/wjst.2021.7306

Authors

Utumporn NGIVPROM Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
Nipa MILINTAWISAMAI Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand http://orcid.org/0000-0001-8806-1091
Alissara REUNGSANG Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

DOI:

https://doi.org/10.48048/wjst.2021.7306

Keywords:

Bioremediation, 1,2-Dichloroethane, Halorespiring bacteria, Reductive dihaloelimination, Vulcanibacillus

Abstract

Bioremediation has been widely used for clean-up of 1,2-dichloroethane (DCA) at contaminated sites. Only a small number of specific anaerobes, halorespiring bacteria (HRB), have been reported to degrade DCA. The goals of this research were the screening and isolation of HRB with capable dechlorination of DCA. HRB were screened and isolated from 7 enrichment cultures (ES1-ES7), from DCA-contaminated soils, on bicarbonate-buffered basal salts medium containing 10 mM acetate and 250 µM DCA under anaerobic conditions and analyzed by gas chromatography. The results showed that the mixed cultures of ES3 and ES5 could reductively dechlorinate DCA to ethylene via a direct reductive dihaloelimination pathway. In particular, ES5 showed rapid transformation of 250 µM DCA to ethylene within 6 days at 30 °C. Specific microbial populations of Vulcanibacillus spp., elucidated by polymerase chain reaction-denaturing gradient gel electrophoresis, were found only in ES3 and ES5, which was related to reductive dihaloelimination activities on DCA. A 16S rRNA gene analysis of isolate es5d8 from mixed culture ES5 revealed 2 strains of Vulcanibacillus spp. (KKU-DCA1 and KKU-DCA2) concerned with dechlorinating DCA. These findings suggested that Vulcanibacillus spp. were HRB that have the potential for detoxifying DCA.

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References

WF Carroll Jr, TC Berger, PJ Borrelli, RA Garrity, RA Jacobs, JW Lewis, RL McCreedy, DR Tuhovak and AF Weston. Characterization of emissions of dioxins and furans from ethylene dichloride (DCE), vinyl chloride (VCM) and polyvinyl chloride (PVC). Chemosphere 1998; 37 1957-72.

Merchant Research Consulting. North America ranks first in worldwide ethylene dichloride (EDC) market, Available at: https://mcgroup.co.uk/news/20141121/north-america-ranks-worldwide-ethylene-dichloride-edc-market.html, accessed June 2018.

Honest Version: The global ethylene dichloride (EDC) market is envisioned to be valued at 58 Million tons by 2027, according to Research Nester, Available at: https://honestversion.com/the-global-ethylene-dichloride-edc-market-is-envisioned-to-be-valued-at-58-million-tons-by-2027-according-to-research-nester, accessed June 2018.

L Fang, C Norris, K Johnson, X Cui, J Sun, Y Teng, E Tian, W Xu, Z Li, J Mo, JJ Schauer, M Black, M Bergin, J Zhang and Y Zhang. Toxic volatile organic compounds in 20 homes in Shanghai: concentrations, inhalation health risks, and the impacts of household air cleaning. Build. Environ. 2019; 157, 309-18.

B Wu, H Li, X Du, L Zhong, B Yang, P Du, Q Gu and F Li. Correlation between DNAPL distribution area and dissolved concentration in surfactant enhanced aquifer remediation effluent: a two-dimensional flow cell study. Chemosphere 2016; 144, 2142-9.

JE Barbash and M Reinhard. Abiotic dehalogenation of 1,2-dichloroethane and 1,2-dibromoethane in aqueous solution containing hydrogen sulfide. Environ. Sci. Technol. 1989; 23, 1349-58.

C Dai, Y Zhou, H Peng, S Huang, P Qin, J Zhang, Y Yang, L Luo and X Zhang. Current progress in remediation of chlorinated volatile organic compounds: A review. J. Ind. Eng. Chem. 2018; 62, 106-19.

A Dzionek, D Wojcieszyńska and U Guzik. Natural carriers in bioremediation: A review. Electron. J. Biotechnol. 2016; 23, 28-36.

CC Azubuike, CB Chikere and GC Okpokwasili. Bioremediation techniques-classification based on site of application: Principles, advantages, limitations and prospects. World J. Microbiol. Biotechnol. 2016; 32, 180.

DW Major, ML McMaster, EE Cox, EA Edwards, SM Dworatzek, ER Hendrickson, MG Starr, JA Payne and LW Buonamici. Field demonstration of successful bioaugmentation to achieve dechlorination of tetrachloroethene to ethene. Environ. Sci. Technol. 2002; 36, 5106-16.

A Roy, A Dutta, S Pal, A Gupta, J Sarkar, A Chatterjee, A Saha, P Sarkar, P Sar and SK Kazy. Biostimulation and bioaugmentation of native microbial community accelerated bioremediation of oil refinery sludge. Bioresour. Technol. 2018; 253, 22-32.

H Smidt and WM de Vos. Anaerobic microbial dehalogenation. Annu. Rev. Microbiol. 2004; 58, 43-73.

Y Sung, KE Fletcher, KM Ritalahti, RP Apkarian, N Ramos-Hernández, RA Sanford, NM Mesbah and FE Löffler. Geobacter lovleyi sp. nov. Strain SZ, a novel metal-reducing and tetrachloroethene-dechlorinating bacterium. Appl. Environ. Microbiol. 2006; 72, 2775-82.

ML Luijten, J de Weert, H Smidt, HT Boschker, WM de Vos, G Schraa and AJ Stams. Description of Sulfurospirillum halorespirans sp. nov., an anaerobic, tetrachloroethene-respiring bacterium, and transfer of Dehalospirillum multivorans to the genus Sulfurospirillum as Sulfurospirillum multivorans comb. nov. Int. J. Syst. Evol. Microbiol. 2003; 53, 787-93.

J Hata, N Miyata, ES Kim, K Takamizawa and K Iwahori. Anaerobic degradation of cis-1,2-dichloroethylene and vinyl chloride by Clostridium sp. strain DC1 isolated from landfill leachate sediment. J. Biosci. Bioeng. 2004; 97, 196-201.

FE Loffler, J Yan, KM Ritalahti, L Adrian, EA Edwards, KT Konstantinidis, JA Muller, H Fullerton, SH Zinder and AM Spormann. Dehalococcoides mccartyi gen. nov., sp. nov., obligately organohalide-respiring anaerobic bacteria relevant to halogen cycling and bioremediation, belong to a novel bacterial class, Dehalococcoidia classis nov., order Dehalococcoidales ord. nov. and family Dehalococcoidaceae fam. nov., within the phylum Chloroflexi. Int. J. Syst. Evol. Microbiol. 2013; 63, 625-35.

C Egli, R Scholtz, AM Cook and T Leisinger. Anaerobic dechlorination of tetrachloromethane and 1,2-dichloroethane to degradable products by pure cultures of Desulfobacterium sp. and Methanobacterium sp. FEMS Microbiol. Lett. 1987; 43, 257-61.

SD Wildeman, G Diekert, HV Langenhove and W Verstraete. Stereoselective microbial dehalorespiration with vicinal dichlorinated alkanes. Appl. Environ. Microbiol. 2003; 69, 5643-7.

M Schmidt, S Lege and I Nijenhuis. Comparison of 1,2-dichloroethane, dichloroethene and vinyl chloride carbon stable isotope fractionation during dechlorination by two Dehalococcoides strains. Water Res. 2014; 52, 146-54.

J He, VF Holmes, PK Lee and L Alvarez-Cohen. Influence of vitamin B12 and cocultures on the growth of Dehalococcoides isolates in defined medium. Appl. Environ. Microbiol. 2007; 73, 2847-53.

Environmental Protection Agency, United States. National primary drinking water regulations, Available at: https://www.epa.gov/sites/production/files/2016-06/documents/npwdr_complete_ table.pdf, accesses June 2018.

FE Loffler, RA Sanford and JM Tiedje. Initial characterization of a reductive dehalogenase from Desulfitobacterium chlororespirans Co23. Appl. Environ. Microbiol. 1996; 62, 3809-13.

EA Wolin, MJ Wolin and RS Wolfe. Formation of methane by bacterial extracts. J. Biol. Chem. 1963; 238, 2882-6.

JM Gossett. Measurement of Henry’s law constants for C1 and C2 chlorinated hydrocarbons. Environ. Sci. Technol. 1987; 21, 202-8.

CH Nakatsu, V Torsvik and L Øvreas. Soil community analysis using DGGE of 16S rDNA polymerase chain reaction products. Soil Sci. Soc. Am. J. 2000; 64, 1382-8.

WG Weisburg, SM Barns, DA Pelletier and DJ Lane. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 1991; 173, 697-703.

S L'Haridon, ML Miroshnichenko, NA Kostrikina, BJ Tindall, S Spring, P Schumann, E Stackebrandt, EA Bonch-Osmolovskaya and C Jeanthon. Vulcanibacillus modesticaldus gen. nov., sp. nov., a strictly anaerobic, nitrate-reducing bacterium from deep-sea hydrothermal vents. Int. J. Syst. Evol. Microbiol. 2006; 56, 1047-53.

JD Thompson, DG Higgins and TJ Gibson. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22, 4673-80.

N Saitou and M Nei. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 1987; 4, 406-25.

J Felsenstein. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 1985; 39, 783-91.

A Hagenauer, H Hippe and FA Rainey. Desulfotomaculum aeronauticum sp. nov., a sporeforming, thiosulfate-reducing bacterium from corroded aluminium alloy in an aircraft. Syst. Appl. Microbiol. 1997; 20, 65-71.

S Berlendis, M Ranchou-Peyruse, ML Fardeau, JF Lascourrèges, M Joseph, B Ollivier, T Aüllo, D Dequidt, M Magot and A Ranchou-Peyruse. Desulfotomaculum aquiferis sp. nov. and Desulfotomaculum profundi sp. nov., isolated from a deep natural gas storage aquifer. Int. J. Syst. Evol. Microbiol. 2016; 66, 4329-38.

X Maymó-Gatell, T Anguish and SH Zinder. Reductive dechlorination of chlorinated ethenes and 1, 2-dichloroethane by Dehalococcoides ethenogenes 195. Appl. Environ. Microbiol. 1999; 65, 3108-13.

K Mukherjee, KS Bowman, FA Rainey, S Siddaramappa, JF Challacombe and WM Moe. Dehalogenimonas lykanthroporepellens BL-DC-9T simultaneously transcribes many rdhA genes during organohalide respiration with 1,2-DCA, 1,2-DCP, and 1,2,3-TCP as electron acceptors. FEMS Microbiol. Lett. 2014; 354, 111-8.