Proteomics of Seed Nutrition-Associated Proteins in Germinated Brown Rice in Four Thai Rice Cultivars Analyzed by GeLC-MS/MS

Authors

  • Sarunyaporn MAKSUP Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand http://orcid.org/0000-0001-8867-3274
  • Sarintip PONGPAKPIAN Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand
  • Sittiruk ROYTRAKUL National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani 12120, Thailand

DOI:

https://doi.org/10.48048/wjst.2021.6953

Keywords:

Germinated brown rice, Hom Nil (HN), Phenolic compounds, Riceberry (RB), Shotgun proteomics

Abstract

Brown rice (BR) and germinated brown rice (GBR) of Oryza sativa L. are popularly consumed by Asians because of their important healthy diet components. They are known to contain bioactive compounds and nutrients, such as phenolics, vitamins, fatty acids, and sugars, which help to maintain good health and reduce the incidences of various chronic diseases. The objective of this study was to investigate the effects of germination on changes of nutrition-associated proteins in 4 rice cultivars. After germination for 24 h, the changes of seed nutrition-associated proteins were examined by shotgun proteomics. The total proteins from non-germinated seeds and 24 h germinated seeds of 4 rice cultivars were extracted and analyzed by in-gel digestion coupled with tandem mass spectrometry (GeLC-MS/MS). A total phenolic content was analyzed from the crude methanol extract of those grains after germination for 0, 24, and 48 h using Folin-Ciocalteu assay. The analysis showed that seed nutritional-associated proteins, especially phenolic-associated proteins, increased after germination according to the accumulation of the total phenolic content. The expressions of 6 phenolic-associated proteins, including phenylalanine ammonia-lyase, serine carboxypeptidase-like protein, isoflavone-7-O-methyltransferase, isoflavonoid glucosyltransferase, glycosyltransferase family 61 protein and UDP-glucose flavonoid 3-O-glucosyltransferase were increased by 2.20 - 15.90 folds after germination. This study provides evidence that rice germination for 24 h has essentially influenced the increased nutritional values of BR and the phenolic biosynthetic pathway.

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References

HY Park, KW Lee and HD Choi. Rice bran constituents: Immunomodulatory and therapeutic activities. Food Funct. 2017; 8, 935-43.

P Goufo and H Trindade. Rice antioxidants: Phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, γ-oryzanol, and phytic acid. Food Sci. Nutr. 2014; 2, 75-104.

G Zhang, VS Malik, A Pan, S Kumar, MD Holmes, D Spiegelman, X Lin and FB Hu. Substituting brown rice for white rice to lower diabetes risk: A focus-group study in Chinese adults. J. Am. Diet. Assoc. 2010; 110, 1216-21.

MU Imam, A Ishaka, DJ Ooi, NDM Zamri, N Sarega, M Ismail and NM Esa. Germinated brown rice regulates hepatic cholesterol metabolism and cardiovascular disease risk in hypercholesterolaemic rats. J. Funct. Foods 2014; 8, 193-203.

F Cornejo, PJ Caceres, C Martínez-Villaluenga, CM Rosell and J Frias. Effects of germination on the nutritive value and bioactive compounds of brown rice breads. Food Chem. 2015; 173, 298-304.

DH Cho and ST Lim. Germinated brown rice and its bio-functional compounds. Food Chem. 2016; 196, 259-71.

S Maksup, S Pongpakpian, S Roytrakul, S Cha-Um and K Supaibulwatana. Comparative proteomics and protein profile related to phenolic compounds and antioxidant activity in germinated Oryza sativa ‘KDML105’ and Thai brown rice ‘Mali Daeng’ for better nutritional value. J. Sci. Food Agr. 2018; 98, 566-73.

D He and P Yang. Proteomics of rice seed germination. Front. Plant Sci. 2013; 4, 1-9.

YT Lin, CC Pao, ST Wu and CY Chang. Effect of different germination conditions on antioxidative properties and bioactive compounds of germinated brown rice. Biomed. Res. Int. 2015; 2015, 608761.

GM Forster, K Raina, A Kumar, S Kumar, R Agarwal, MH Chen, JE Bauer, AM McClung and EP Ryan. Rice varietal differences in bioactive bran components for inhibition of colorectal cancer cell growth. Food Chem. 2013; 141, 1545-52.

C Kozuka, K Yabiku, C Takayama, M Matsushita, M Shimabukuro and H Masuzaki. Natural food science based novel approach toward prevention and treatment of obesity and type 2 diabetes: Recent studies on brown rice and γ-oryzanol. Obes. Res. Clin. Pract. 2013; 7, 165-72.

F Wu, N Yang, A Toure, Z Jin and X Xu. Germinated brown rice and its role in human health. Crit. Rev. Food Sci. 2013; 53, 451-63.

NH Azmi, M Ismail, N Ismail, MU Imam, NBM Alitheen and MA Abdullah. Germinated brown rice alters A(1-42) aggregation and modulates Alzheimer’s disease-related genes in differentiated human SH-SY5Y cells. Evid. Based Compl. Alt. 2015; 2015, 153684.

P Xi and RH Liu. Whole food approach for type 2 diabetes prevention. Mol. Nutr. Food Res. 2016; 60, 1819-36.

C Perez-Ternero, MA de Sotomayor and MD Herrera. Contribution of ferulic acid, γ-oryzanol and tocotrienols to the cardiometabolic protective effects of rice bran. J. Funct. Foods 2017; 32, 58-71.

MH Chen, AM McClung and CJ Bergman. Phenolic content, anthocyanins and antiradical capacity of diverse purple bran rice genotypes as compared to other bran colors. J. Cereal Sci. 2017; 77, 110-9.

Y Pang, S Ahmed, Y Xu, T Beta, Z Zhu, Y Shao and J Bao. Bound phenolic compounds and antioxidant properties of whole grain and bran of white, red and black rice. Food Chem. 2018; 240, 212-21.

AHM Kamal, KH Kim, KH Shin, JS Choi, BK Baik, H Tsujimoto, HY Heo, CS Park and SH Woo. Abiotic stress responsive proteins of wheat grain determined using proteomics technique. Aust. J. Crop Sci. 2010; 4, 196-208.

BC Tan, YS Lim and SE Lau. Proteomics in commercial crops: An overview. J. Proteom. 2017; 169, 176-88.

SJ Liu, HH Xu, WQ Wang, N Li, WP Wang, Z Lu, IM Møller and SQ Song. Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds. J. Plant Physiol. 2016; 196, 79-92.

HH Xu, SJ Liu, SH Song, RX Wang, WQ Wang and SQ Song. Proteomics analysis reveals distinct involvement of embryo and endosperm proteins during seed germination in dormant and non-dormant rice seeds. Plant Physiol. Bioch. 2016; 103, 219-42.

F Granier. Extraction of plant proteins for two dimensional electrophoresis. Electrophoresis 1988; 9, 712-8.

S Maksup, S Roytrakula and K Supaibulwatana. Physiological and comparative proteomic analyses of Thai jasmine rice and two check cultivars in response to drought stress. J. Plant Interact. 2014; 9, 43-55.

OH Lowry, NJ Rosebrough, AL Farr and RJ Randall. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951; 193, 265-75.

J Jaresitthikunchai, N Phaonakrop, S Kittisenachai and S Roytrakul. Rapid in-gel digestion protocol for protein identification by peptide mass fingerprint. In: Proceedings of the 2nd Biochemistry and Molecular Biology Conference: Biochemistry and Molecular Biology for Regional Sustainable Development, Khon Kaen, Thailand. 2009, p. 29.

C Johansson, J Samskog, L Sundstrom, H Wadensten, L Bjorkestena and J Flensburg. Differential expression analysis of Escherichia coli proteins using a novel software for relative quantitation of LC-MS/MS data. Proteomics 2006; 6, 4475-85.

A Thorsell, E Portelius, K Blennow and BA Westman. Evaluation of sample fractionation using microscale liquid-phase isoelectric focusing on mass spectrometric identification and quantitation of proteins in a SILAC experiment. Rapid Commun. Mass Sp. 2007; 21, 771-8.

DN Perkins, DJC Pappin, DM Creasy and JS Cottrell. Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 1999; 20, 3551-67.

B Ura, F Scrimin, G Arrigoni, C Franchin, L Monasta and G Ricci. A proteomic approach for the identification of up-regulated proteins involved in the metabolic process of the leiomyoma. Int. J. Mol. Sci. 2016; 17, 1-16.

P Bardou, J Mariette, F Escudié, C Djemiel and C Klopp. jvenn: An interactive Venn diagram viewer. BMC Bioinformatics 2014; 15, 293-9.

J Sutharuta and J Sudarat. Total anthocyanin content and antioxidant activity of germinated colored rice. Int. Food Res. J. 2012; 19, 215-21.

U Umnajkitikorn, B Faiyue and K Saengnil. Enhancing antioxidant properties of germinated Thai rice (Oryza sativa L.) cv. Kum Doi Saket with salinity. J. Rice Res. 2013; 1, 1-8.

NN Htwe, V Srilaong, K Tanprasert, A Uthairatanakija and S Kanlayanarat. Characterization of bioavailable compounds in black and red pigmented rice cultivars in Thailand. Acta Hortic. 2009; 837, 65-72.

M Peanparkdee, S Iwamoto and R Yamauchi. Preparation and release behavior of gelatin-based capsules of antioxidants from ethanolic extracts of Thai Riceberry bran. Food Bioprocess Tech. 2017; 10, 1737-48.

V Leardkamolkarn, W Thongthep, P Suttiarporn, R Kongkachuichai, S Wongpornchai and A Wanavijitr. Chemopreventive properties of the bran extracted from a newly-developed Thai rice: The Riceberry. Food Chem. 2011; 125, 978-85.

P Arjinajarn, N Chueakula, A Pongchaidecha, K Jaikumkao, V Chatsudthipong, S Mahatheeranont, O Norkaew, N Chattipakorn and A Lungkaphin. Anthocyanin-rich Riceberry bran extract attenuates gentamicin-induced hepatotoxicity by reducing oxidative stress, inflammation and apoptosis in rats. Biomed. Pharmacother. 2017; 92, 412-20.

A Suksomboon, K Limroongreungrat, A Sangnark, K Thititumjariya and A Noomhorm. Effect of extrusion conditions on the physicochemical properties of a snack made from purple rice (Hom Nil) and soybean flour blend. Int. J. Food Sci. Tech. 2011; 46, 201-8.

D Ravaglia, RV Espley, RA Henry-Kirk, C Andreotti, V Ziosi, RP Hellens, G Costa and AC Allan. Transcriptional regulation of flavonoid biosynthesis in nectarine (Prunus persica) by a set of R2R3 MYB transcription factors. BMC Plant Biol. 2013; 13, 68-82.

X Zhang and CJ Liu. Multifaceted regulations of gateway enzyme phenylalanine ammonia-lyase in the biosynthesis of phenylpropanoids. Mol. Plant 2015; 8, 17-27.

CM Fraser, MG Thompson, AM Shirley, J Ralph, JA Schoenherr, T Sinlapadech, MC Hall and C Chapple. Related arabidopsis serine carboxypeptidase-like sinapoylglucose acyltransferases display distinct but overlapping substrate specificities. Plant Physiol. 2007; 144, 1986-99.

A Noguchi, A Saito, Y Homma, M Nakao, N Sasaki, T Nishino, S Takahashi and T Nakayama. 2007. A UDP-glucose: Isoflavone7-O-glucosyltransferase from the roots of soybean (Glycine max) seedlings. Purification, gene cloning, phylogenetics, and an implication for an alternative strategy of enzyme catalysis. J. Biol. Chem. 2007; 282, 23581-90.

BK Franzmayr, S Rasmussen, KM Fraser and PE Jameson. Expression and functional characterization of a white clover isoflavone synthase in tobacco. Ann. Bot. London 2012; 110, 1291-301.

A Trapero, O Ahrazem, A Rubio-Moraga, ML Jimeno, MD Gómez and L Gómez-Gómez. Characterization of a glucosyltransferase enzyme involved in the formation of kaempferol and quercetin sophorosides in Crocus sativus. Plant Physiol. 2012; 159, 1335-54.

N Anders, MD Wilkinson, A Lovegrove, J Freeman, T Tryfona, TK Pellny, T Weimar, JC Mortimer, K Stott, JM Baker, M Defoin-Platel, PR Shewry, P Dupree and RA Mitchell. Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses. Proc. Natl. Acad. Sci. 2012; 109, 989-93.

ZX Lu, KZ Walker, JG Muir and K O'Dea. Arabinoxylan fibre improves metabolic control in people with type II diabetes. Eur. J. Clin. Nutr. 2004; 58, 621-8.

MS Izydorczyk and JE Dexter. Barley β-glucans and arabinoxylans: molecular structure, physicochemical properties, and uses in food products. Food Res. Int. 2008; 41, 850-68.

LN Malunga, M Izydorczyk and T Beta. Antiglycemic effect of water extractable arabinoxylan from wheat aleurone and bran. J. Nutr. Metab. 2017; 2017, 5784759.

LN Malunga and T Beta. Antioxidant capacity of arabinoxylan oligosaccharide fractions prepared from wheat aleurone using Trichoderma viride or Neocallimastix patriciarum xylanase. Food Chem. 2015; 167, 311-9.

AP Tuan, S Zhao, KJ Kim, BY Kim, J Yang, HC Li, SJ Kim, VM Arasu, AN Al-Dhabi and US Park. Riboflavin accumulation and molecular characterization of cDNAs encoding bifunctional GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase, lumazine synthase, and riboflavin synthase in different organs of lycium chinense plant. Molecules 2014; 19, 17141-53.

RC Clough, AL Matthis, SR Barnum and JG Jaworski. Purification and characterization of 3-ketoacyl-acyl carrier protein synthase III from spinach. A condensing enzyme utilizing acetyl-coenzyme A to initiate fatty acid synthesis. J. Biol. Chem. 1992; 267, 20992-8.

J Guo, X Ma, Y Cai, Y Ma, Z Zhan, YJ Zhou, W Liu, M Guan, J Yang, G Cui, L Kang, L Yang, Y Shen, J Tang, H Lin, X Ma, B Jin, Z Liu, RJ Peters, ZK Zhao and L Huang. Cytochrome P450 promiscuity leads to a bifurcating biosynthetic pathway for tanshinones. New Phytol. 2016; 210, 525-34.

Z Wang, H Li, M Liang and L Yang. Glutelin and prolamin, different components of rice protein, exert differently in vitro antioxidant activities. J. Cereal Sci. 2016; 72, 108-16.

G Schenk, N Mitić, GR Hanson and P Comba. Purple acid phosphatase: a journey into the function and mechanism of a colorful enzyme. Coordin. Chem. Rev. 2013; 257, 473-82.

MU Imam, NH Azmi, MI Bhanger, N Ismail, M Ismail. Antidiabetic properties of germinated brown rice: A systematic review. Evid. Based Compl. Alt. 2012; 2012, 816501.

B Min, AMM Clung and MH Chen. Phytochemicals and antioxidant capacities in rice brans of different color. J. Food Sci. 2011; 76, 117-26.

B Min, L Gu, AMM Clung, CJ Bergman and MH Chen. Free and bound total phenolic concentrations, antioxidant capacities, and profiles of proanthocyanidins and anthocyanins in whole grain rice (Oryza sativa L.) of different bran colours. Food Chem. 2012; 133, 715-22.

KN Jom, Y Lorjaroenphon and P Udompijitkul. Differentiation of four varieties of germinating Thai colored indica rice (Oryza sativa L.) by metabolite profiling. Food Sci. Tech. Res. 2016; 22, 65-73.

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Published

2021-01-01

How to Cite

MAKSUP, S. ., PONGPAKPIAN, S. ., & ROYTRAKUL, S. . (2021). Proteomics of Seed Nutrition-Associated Proteins in Germinated Brown Rice in Four Thai Rice Cultivars Analyzed by GeLC-MS/MS . Walailak Journal of Science and Technology (WJST), 18(1), Article 6953 (13 pages). https://doi.org/10.48048/wjst.2021.6953