Effect of Copper on the Humoral and Biochemical Indices of the Teleost fish, Anabas testudineus (Bloch, 1792)

Bhaskaran Nair Girish KUMAR, Sivasankaran Bijoy NANDAN

Abstract


The effects of sub-lethal concentrations of copper and the ameliorating capacity of vitamin C on the humoral and biochemical parameters in the teleost fish, Anabas testudineus, were investigated. The 96 h LC 50 value of copper for A. testudineus was determined by Probit method and was found to be 1.74 mg/L. The blood of fish from two sub-lethal concentrations along with vitamin supplemented media and control medium, were analysed. The haemoglobin (Hb), haematocrit (Hct), erythrocyte count (RBC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC) were reduced (p > 0.0001) in copper exposed fish compared to the control. The leucocyte (WBC) count increased (p < 0.0001) with dose and duration. Immature and amitotic RBCs, hypoxia, hypochromia, and crenation were observed in highest nominal concentrations and last exposures. The plasma glucose, cortisol, GOT (Glutamate oxalate transaminase), GPT (Glutamate pyruvate transaminase), ALP (Alkaline phosphatase) and LDH (lactate dehydrogenase) increased (p < 0.0001), and serum protein decreased. However, on day 28, the Hb, RBC count, MCH, MCHC, and the oxygen carrying capacity increased, and glucose, cortisol and LDH decreased, markedly. The administration of vitamin C improved the blood parameters, illustrating its prophylactic capacity to overwhelm the trace metal stress.

doi:10.14456/WJST.2014.78


Keywords


Copper, vitamin C, antioxidant capacity, haematological and biochemical parameters, Anabas testudineus

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References


A Meguid, NAM Kheiralleh, K Abu-Shaban, A Adham and A Moneim. Histochemical and biochemical changes in liver of Tilapia Zilli: as a consequence of water pollution. J. Biol. Sci. 2002; 2, 224-9.

JO Nriagu. A global assessment of natural sources of atmospheric trace metals. Nature 1998; 338, 47-9.

PP Ciji. 2012, Toxicity due to Copper and Zinc on the Freshwater Fish, Puntius parrah. Ph. D. Dissertation. Mahatma Gandhi University, Kottayam, Kerala, India.

EM Sorenson. Metal Poisoning in Fish. Environmental and Life Science Associates, CRC Press, Broca Raton, 1991, p. 1-374.

JB Sprague. The ABC’s of Pollutant Bioassay using Fish. Biological Methods for the Assessment of Water Quality. ASTM STP, 1973, p. 6-30.

DJ Finney. Probit Analysis. Cambridge University Press, Cambridge, UK, 1971.

I Davidson and JB Henry. Todd-Sanford Clinical Diagnosis by Laboratory Methods. Philadelphia, Pennsylvania, W. B. Saunders Company, 1969, p. 1165.

D Hunter and RR Bomford. Hutchison’s Clinical Methods. Publ. Bailier Tindall and Casell, London, 1969, p. 1-145.

JD Cook. Measurement of Iron Status. A report of the International Anaemia Consultive Group (INACG), New York, Washington DC, 1985, p. 4.

MM Wintrobe. Clinical Haematology. Henry Kimpton, London, 1997, p. 1-448.

JV Dacie and SM Lewis. Practical Haematology. Churchill Living Stone, Edinburgh, London, 1975.

L Thomas. Clinical Chemistry Diagnostics. Frankfurt, 1998.

HM Levesque. Endocrine Tests and Radioimmuno Assay. In: R Sood (ed.). Estimation of Plasma Cortisol. 4th ed. Medical Laboratory Technology, Methods and Interpretation, 1994, p. 367.

R Rej and RE Vanderlinde. Assay of aspartate aminotransferase activity: effects of serum and serum proteins on oxalacetate decarboxylationand dialysis. Clin. Chem. 1974; 20, 454-64.

PL Wolf, D Williams, N Coplon and AS Coulson. Low ALT activity in recovering patients undergoing chronic haemodialysis. Clin. Chem. 1972, 18, Article ID 567.

OA Bessey, OH Lowry and MJ Brock. A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. J. Biol. Chem. 1946; 164, 321-9.

OH Lowry, NJ Ronebrough, AI Farr and RJ Randall. Protein measurement with Folin-Phenol reagent. J. Biol. Chem. 1951; 193, 265-76.

P Trinder. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann. Clin. Biochem. 1969, 6, 24-7.

GK Iwama, GL Greer and PA Larkin. Changes in some haematological characteristics of coho salmon in response to acute exposure of dehydro ascorbic acid at different exercise levels. J. Fish. Res. Board Can. 1976; 33, 285-9.

SB Nandan and PT Nimila. Lindane toxicity: Histopathological, behavioural and biochemical changes in Etroplus maculatus. Mar. Environ. Res. 2011; 30, 1-8.

D Aranudova, A Aranudova and E Tomova. Selected haematological indices of freshwater fish from Studen Kladenetsh reservoir. Bulg. J. Agri. Sci. 2008; 14, 244-50.

D Singh, K Nath, SP Trivedi and YK Sharma. Impact of copper on haemological profile of freshwater fish, Channa punctatus. J. Environ. Biol. 2008; 29, 253-7.

RV Zikic and S Stajn. Activities of superoxide dismutase and catalase in RBC and plasma transaminases of goldfish (Carassius auratus gibelio) exposed to cadmium. Physiol. Res. 2001; 50, 105-11.

S Ravichandran, M Shanthi and N Indira. Effect of phenol on blood glucose level of freshwater fish, Oreochromis mossambicus. Environ. Ecol. Stat. 1995; 13, 129-31.

P Shahbazi and N Maleknia. General Biochemistry. Tehran University Press, 1999, p. 465-88.

LEW Flodmark, MA Urke, JH Halleraer, JV Arnekleiv, IA Vollestad and ABS Poleo. Cortisol and glucose responses in juvenile brown trout subjected to a fluctuating flow regime in an artificial stream. J. Fish Biol. 2002; 60, 238-48.

BB Hosetti, N Praveen, N Dube and A Shwetha. Metabolic changes in the freshwater fish, Catla catla under copper cyanide intoxication. Int. J. Pharma. Biol. Arch. 2011; 2, 874-9.

AME Shalaby. The opposing effect of ascorbic acid on ochratoxin toxicity in Nile tilapia. In: Proceedings of the 6th International Symposium on Tilapia in Aqua Culture. American Tilapia Association, Pine Bluff, AR, 2004, p. 209-21.

P Parthiban and M Muniyan. Effect of heavy metal nickel on the lipid peroxidation and antioxidant parameters in the liver tissue of Cirrhinus mrigala. Int. J. Dev. Res. 2011; 1, 1-4.

O Firat, HY Cogun, TA Yuzereroglu, G Gok, O Firat, F Kargin and Y Koteman. A comparative study on the effects of a pesticide (cypermethrin) and two metals (copper and lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus. Fish Physiol. Biochem. 2011; 37, 657-66.


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