Callus Induction and Phytochemical Constituents of Finger Eggplant (Solanum sp.)

Authors

  • Norizzah Jaafar SIDIK Department of Biology, Faculty of Applied Science, Universiti Teknologi MARA, Selangor, Malaysia
  • Norhayati DAUD Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Perak Darul Ridzuan, Malaysia
  • Som Cit SINANG Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Perak Darul Ridzuan, Malaysia
  • Nurul Fazira OMAR Department of Biology, Faculty of Applied Science, Universiti Teknologi MARA, Selangor, Malaysia

DOI:

https://doi.org/10.48048/wjst.2021.6757

Keywords:

Solanum sp., Finger eggplant, NAA, BAP, Callus, Phytochemical constituent

Abstract

This study examined the efficiency of callus induction on optimum concentrations of NAA (a-naphthaleneacetic acid) and BAP (6-benzyladenine) from culturing stem and leaf explants of finger eggplant (Solanum sp.) and investigated the phytochemical constituents of callus tissue. Seeds were sterilized by using 3 and 5 % Clorox solution, which gave the highest number of survival seeds (100 %) and were grown in vitro plantlets. The highest frequency of callus induction (100.00 ± 0.00 %) was obtained from stems and leaf explants that were excised from in vitro plantlets. The stem explants cultured on MS medium consisted of 1.0 mg/L NAA + 1.0 mg/L BAP, giving the maximum mean callus fresh weight (0.14 ± 0.05 g). Meanwhile, the leaf explants cultured on MS medium consisted of  0.5 mg/L NAA + 2.0 mg/L BAP, generating the maximum mean callus fresh weight (0.48 ± 0.10 g). The highest frequency of callus induction (88.00 ± 1.60 %) was obtained in solidified MS medium supplemented with 0.5 mg/L NAA + 2.0 mg/L BAP, producing the maximum mean fresh weight of callus (1.54 ± 0.27 g) and dry weight (0.90 ± 0.01 g). The results of the Phytochemical screenings of callus and dried leaf extracts indicated the presence of alkaloids, flavonoids, terpenoids, and saponins.

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Published

2021-01-01

How to Cite

SIDIK, N. J. ., DAUD, N. ., SINANG, S. C. ., & OMAR, N. F. . (2021). Callus Induction and Phytochemical Constituents of Finger Eggplant (Solanum sp.). Walailak Journal of Science and Technology (WJST), 18(1), Article 6757 (12 pages). https://doi.org/10.48048/wjst.2021.6757