Effects of Curcuma comosa Roxb. Extract on the Expression of CYP450s in Immortalized Hepatocyte-like Cells (imHC)

Petdao  PETCHUAY; Yindee KITIYANANT; Patoomratana TUCHIDA; Suradej HONGENG; Khanit SA-NGIAMSUNTORN; Vichien KEERATINIJAKAL; Suparerk BORWORNPINYO; Noppawan Phumala MORALES

doi:10.48048/wjst.2021.6704

Authors

Petdao PETCHUAY Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Yindee KITIYANANT Department of Anatomy, Faculty of Science, Mahidol UniversityDepartment of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Patoomratana TUCHIDA Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Suradej HONGENG Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand
Khanit SA-NGIAMSUNTORN Department of Biochemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand
Vichien KEERATINIJAKAL Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
Suparerk BORWORNPINYO Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Noppawan Phumala MORALES Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

DOI:

https://doi.org/10.48048/wjst.2021.6704

Keywords:

Curcuma comosa Roxb., Xenobiotic, Detoxification, Immortalized hepatocyte-like cell, Cytochrome P450

Abstract

Hepatocytes are the most abundant liver cells that produce biotransformation enzymes, including phase I metabolism enzymes and cytochrome P450s (CYP450s). These enzymes are induced or suppressed by several drugs and chemicals. Here, immortalized hepatocyte-like cells (imHC) were applied as a novel hepatocyte to study the CYP450 enzyme expression induced by a Thai folk herb, Curcuma comosa Roxb. (C. comosa). The results demonstrated that the mRNA expression levels were dependent on cell cultivation conditions, the extract's concentration, and the duration of treatment. In the 3-day cultivation, 1 µg/mL C. comosa extract upregulated the mRNA expression levels of CYP1A1, CYP1A2, CYP2B6, CYP2C9, CYP2D6, CYP2E1 and CYP3A4 in imHC, after treatment for 48 h, which was comparable to the expression levels of those genes in HepG2 cells. In addition, C. comosa increased CYP3A4 protein expression in both imHC and HepG2 cells. Furthermore, 1 µg/mLC. comosa could neutralize erythromycin's inhibitory effect, a specific CYP3A4 inhibitor, on CYP3A4 enzyme activity. In conclusion, imHC exhibited several detoxification enzymes, and this cell could be used to replace hepatic carcinoma cells in hepatotoxic studies. The function of C. comosa as a CYP enzyme-inducing agent indicates a promising treatment for the prevention of drug-induced hepatotoxicity by activating detoxification enzymes.

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